Kanamycin Esculin Azide (KAA) Agar (AS-1256)

$145.00 AUD

AuSaMicS Life Science • Extreme Selectivity

Kanamycin Esculin Azide Agar

KAA Agar for Group D Streptococci

A specialized selective and differential medium for the isolation and enumeration of Enterococci in food and environmental samples. Dual-action inhibition using Kanamycin and Sodium Azide ensures clean recovery from complex matrices.

AS-1256 🧪 ISO 7899-2 Baseline ✓ Esculin Positive 🛡️ Dual Antibiotic/Chemical Barrier

Selectivity: Azide & Kanamycin Incubation: 37°C / 24h Prep: 42.7g/L Yield

Isolation Specialist

Visual Contrast - Black zones around colonies

High Potency - Inhibits Proteus and Staph

Rapid ID - Confirmation in under 24 hours

Stable Formula - Consistent esculin reaction

Clinical & Food Certified

Validated for Fecal Enterococci

🧪 Selective & Chromogenic Composition

📋 Formulation (per Liter)

Component	Concentration	Role
Tryptone / Yeast Extract	25.0 g	Amino acids & Growth factors
Esculin	1.0 g	Differential substrate
Ferric Ammonium Citrate	0.5 g	Indicator of Esculin hydrolysis
Sodium Azide / Kanamycin	0.17 g	Selective Agents
Bacteriological Agar	15.0 g	Solidifying Agent

Diagnostic Mechanism

Esculin Hydrolysis

Enterococci hydrolyze Esculin to Esculetin, which reacts with Ferric ions to form a Black-Brown phenolic complex.

Selective Pressure

Sodium Azide and Kanamycin synergize to suppress Gram-negative rods and the majority of Gram-positive background flora.

📋 Technical Profile

Catalogue No.	AS-1256
Final pH	7.0 ± 0.2
Yield	42.7g / Liter
Storage	10-25°C, Dry

⚗️ Laboratory Prep

1 Suspend 42.7g in 1L deionized water.
2 Heat to boiling until completely dissolved.
3 Autoclave at 121°C for 15 min. Avoid overheating.

📄 Documentation

📋 View COA ⚠️ SDS