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KF Streptococcus Broth Selective Enrichment & MPN Enumeration Ausamis
  • KF Streptococcus Broth Selective Enrichment & MPN Enumeration Ausamis
KF Streptococcus Broth (AS-1258) | Selective MPN Medium for Enterococci

KF Streptococcus Broth (AS-1258) | Selective MPN Medium for Enterococci

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AuSaMicS Life Science • Water Quality Analysis

KF Streptococcus Broth

Selective Enrichment & MPN Enumeration

A highly selective liquid medium for the detection of faecal streptococci in water and food samples. Optimized for MPN determination and recovery of stressed Enterococcus species.

AS-1258 🔬 TTC Reduction ✓ APHA / FDA-BAM ⚠️ Contains Azide
Indicator: Bromocresol Purple Detection: Red Formazan pH: 7.2 ± 0.2

Diagnostic Indicators

Positive (+)
Deep Red/Maroon + Turbidity
Sodium Azide (0.04%)
Strong Gram-Negative Inhibition
Visual Clarity
Distinct color shift for MPN

🔬 Primary Applications

Water & Wastewater

Traditional MPN method for fecal streptococci enumeration in EPA-approved labs.

Food Microbiology

Enrichment of enterococci from shellfish and heavily contaminated food ingredients.

🧪 Formula Composition (per Liter)

Ingredient Concentration Function
Proteose Peptone No. 3 10.0 g Nitrogen & Carbon Source
Yeast Extract 10.0 g Vitamins & Growth Factors
Maltose 20.0 g Fermentable Carbohydrate
Sodium Azide 0.4 g Selective Agent (Inhibits Gram-)
TTC (Tetrazolium) 0.1 g Redox Indicator (Red pigment)

📋 Technical Specs

Cat Number AS-1258
Yield 56.52 g / L
Storage 2 - 8°C (Prepared)
Shelf Life 2 Weeks (Prepared)

⚠️ Preparation Protocol

HEAT LABILE: DO NOT AUTOCLAVE

  1. 1 Suspend 56.52g in 1L purified water.
  2. 2 Heat gently until dissolved. Do not boil >1 minute.
  3. 3 Dispense into sterile tubes aseptically.
  4. 4 Sterilize via filtration or tyndallization if required.

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