M17 Broth
M17 Broth
Selective Growth Medium for Lactic Acid Bacteria
Catalog Number: AS-1281
Overview
M17 Broth is a specialised liquid culture medium formulated for the cultivation and enumeration of lactic acid bacteria, particularly Streptococcus thermophilus. Enriched with peptones, lactose, and ascorbic acid, the medium supports robust bacterial growth and metabolic activity, making it a preferred choice in dairy microbiology, fermentation research, and starter culture development.
M17 Broth is extensively used in yogurt and cheese production to monitor bacterial performance, assess starter culture activity, and ensure consistent fermentation outcomes.
Applications
• Cultivation and enumeration of lactic acid bacteria in dairy products
• Starter culture performance testing in yogurt and cheese production
• Microbiological research in food fermentation and probiotic studies
• Quality control testing in milk and dairy processing industries
Key Features & Benefits
• Optimised for Streptococcus thermophilus growth
• Supports high-density growth of lactic acid bacteria
• Widely used in dairy fermentation and starter culture evaluation
• Nutrient-dense formulation suitable for routine and research applications
Principle of the Medium
M17 Broth provides a balanced supply of nitrogen, carbohydrates, and growth factors required by lactic acid bacteria. Peptones supply amino acids and peptides, lactose serves as the primary fermentable carbohydrate, and ascorbic acid improves the redox potential of the medium, enhancing growth rate and metabolic activity of fastidious lactic acid bacteria.
Typical Composition
Peptones – source of amino acids and peptides
Lactose – primary carbohydrate for fermentation
Ascorbic Acid – enhances redox conditions and growth performance
(Exact formulation may vary to meet published or institutional specifications.)
Preparation
Prepare the medium according to standard laboratory procedures or applicable reference methods.
Sterilise as required before use.
Cool to room temperature prior to inoculation.
Storage & Stability
Dehydrated medium: Store at 15–30 °C in a dry, tightly sealed container
Prepared medium: Store at 2–8 °C, protected from light
Do not use the medium if signs of contamination or degradation are observed
Intended Use
For laboratory research and industrial microbiology use only.
Not for medical, food, or veterinary therapeutic use.
Quality & Compliance
Manufactured under controlled conditions to ensure batch-to-batch consistency.
Suitable for dairy, fermentation, and food microbiology laboratories.
Performance comparable to leading international culture media suppliers.
Customs & Trade Information
HS / AHECC Code: 3821.00.00
Prepared culture media for the development or maintenance of microorganisms