Malachite Green Broth Base (AS-1285) | Selective Enrichment for Pseudomonas

$75.00 AUD

AuSaMicS Life Science • Selective Enrichment

Malachite Green Broth Base

Selective Recovery of Pseudomonas aeruginosa

An optimized enrichment medium designed for the selective isolation of Pseudomonas aeruginosa from water, clinical specimens, and environmental samples through potent inhibitory action.

AS-1285 🧪 Selective Dye Assay ✓ Water Testing 🔬 High Specificity

Inhibitor: Malachite Green Incubation: 35-37°C pH: 7.3 ± 0.2

Selective Profile

Pseudomonas
Robust Enrichment

Gram-Positive
Strongly Inhibited

Non-Target Gram-Neg
Suppressed by Dye

🔬 Selective Principle

The medium utilizes **Malachite Green** as the primary selective agent. While many bacteria are highly sensitive to the toxic effects of this dye, Pseudomonas aeruginosa exhibits a unique tolerance, allowing it to outcompete background flora during enrichment. The inclusion of balanced peptones ensures rapid recovery of Pseudomonas even from stressed environmental sources.

🧪 Formulation Details (per Liter)

Ingredient	Concentration	Function
Peptone Mixture	15.0 g	Nitrogen & Carbon Source
Meat Extract	3.0 g	Growth Factors & Vitamins
Malachite Green	0.015 g	Selective Inhibitory Dye

📋 Technical Specs

Cat Number	AS-1285
Final pH	7.3 ± 0.2
Yield	18.015 g / L
Storage	2-25°C

⚗️ Laboratory Prep

1 Suspend 18.015g in 1L purified water.
2 Mix well and dispense into final containers.
3 Autoclave at 121°C for 15 minutes.
4 Subculture to selective agar after 24-48h incubation.