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Methylene Blue 1% w/v Solution | AuSaMicS Australia
$25.00 AUD
AuSaMicS Life Science™
Biological Stains & Laboratory Reagents
Cat. No. AS-54001 · CAS 61-73-4
Methylene Blue 1% w/v Aqueous Solution
C₁₆H₁₈ClN₃S · MW 319.85 g/mol · λmax ~664 nm
Ready-to-use laboratory-grade biological stain and reversible redox indicator at 10 mg/mL in high-purity deionised water. Manufactured in Melbourne, Australia. Heavy metals below standard threshold — every batch.
Laboratory Grade
Ready to Use
Heavy Metals Tested
Australian Made
Same-Day Dispatch
TDS · SDS · COA
1%
w/v concentration
Formula Strength
10mg
per millilitre
Working Conc.
664nm
absorption maximum
λmax UV-Vis
3–4.5
at 25°C
pH Range
Made in Melbourne
Thomastown VIC facility
Same-Day Dispatch
Orders before 12 PM AEST
Full Documentation
TDS · SDS · COA every batch
Technical Support
Direct from the formulator
Overview
AuSaMicS Methylene Blue 1% w/v (AS-54001) is a cationic phenothiazine dye prepared at 10 mg/mL in high-purity deionised water. As a biological stain, it binds electrostatically to negatively charged cellular structures — nucleic acids, polysaccharides, and acidic mucopolysaccharides. As a redox indicator, it cycles reversibly between its oxidised (blue) and reduced (colourless leuco) forms.
Local supply advantage Manufactured and quality-tested at our Thomastown VIC facility. Heavy metals tested below standard threshold. COA, TDS and GHS-compliant SDS with every order. Technical queries handled directly by the formulation team.
Staining Mechanism
MB⁺ (oxidised — deep blue) ⇌ Leuco-MB (reduced — colourless)
+ e⁻ / − e⁻ (reversible equilibrium)
Staining: MB⁺ + Nucleic Acids / Polysaccharides
↓ electrostatic binding
Stable Blue Complex
+ e⁻ / − e⁻ (reversible equilibrium)
Staining: MB⁺ + Nucleic Acids / Polysaccharides
↓ electrostatic binding
Stable Blue Complex
The cationic MB⁺ molecule binds strongly to anionic biological structures via electrostatic attraction. The phenothiazine ring system intercalates between nucleic acid base pairs, producing intense, stable blue colouration that resists washing under standard staining protocols.
Applications
Bacterial Cell Staining
Simple and differential staining of bacterial morphology for light microscopy in research and teaching.
Redox Indicator
Reversible electron acceptor for oxidation-reduction studies and anaerobic culture monitoring.
Histology & Cytology
Staining of nucleic acids and acidic cellular components in histological and cytological preparations.
Teaching Laboratories
Ready-to-use format delivers consistent results in university teaching practicals — no preparation needed.
Plant & Aquatic Science
Chloroplast staining and dissolved oxygen studies in botanical and environmental research.
Analytical Chemistry
Redox titration indicator and enzyme activity assays in analytical and biochemical workflows.
Application Protocols
| Application | Dilution | Conditions | Result |
|---|---|---|---|
| Bacterial staining | Undiluted or 1:2 | RT, 30–60 sec | Blue-stained cells |
| Redox indicator | 1–5 drops / 100 mL | Aerobic or anaerobic | Blue → colourless |
| Nucleic acid stain | 1:5–1:10 in buffer | pH 6.8–7.2 | Blue nucleic acids |
| Teaching labs | Undiluted | Standard protocol | Consistent signal |
Cross-Reference / Equivalents
Sigma-Aldrich / Merck
Methylene Blue Solution
M9140 / 03978
Thermo Fisher Scientific
Methylene Blue Aqueous
Cat: M291
HiMedia
Methylene Blue 1% Solution
Cat: MB010
Oxoid / Thermo
Methylene Blue Stain
Cat: BR0131G
Frequently Used Together
Product Identification
| Product Name | Methylene Blue 1% w/v Aqueous Solution |
| Catalogue Number | AS-54001 |
| CAS Number | 61-73-4 |
| IUPAC Name | [7-(Dimethylamino)phenothiazin-3-ylidene]-dimethylazanium chloride |
| Molecular Formula | C₁₆H₁₈ClN₃S |
| Molecular Weight | 319.85 g/mol |
| EC Number | 200-515-2 |
| Synonyms | Methylthioninium chloride; Basic Blue 9; CI 52015 |
Physical & Chemical Properties
| Property | Specification |
|---|---|
| Appearance | Clear, deep blue aqueous solution |
| Concentration | 1% w/v (10 mg/mL) |
| Solvent | High-purity deionised water |
| Absorption Maximum (λmax) | ~664 nm |
| pH (25°C) | 3.0 – 4.5 |
| Density | ~1.00 g/mL at 25°C |
| Heavy Metals | Below standard threshold |
| Storage | 15–25°C, protect from light |
| Shelf Life | 24 months (unopened) |
Quality Control
| Test Parameter | Method | Specification |
|---|---|---|
| Appearance | Visual | Clear deep blue, particle-free |
| Identity (λmax) | UV-Vis Spectrophotometry | ~664 nm — conforms to reference |
| pH | Potentiometric | 3.0 – 4.5 at 25°C |
| Heavy Metals | ICP / AAS or equivalent | Below standard threshold |
Literature References
- Wainwright M. (2008). Methylene blue derivatives — suitable photoantimicrobials for blood product disinfection? Int J Antimicrobial Agents, 32(6), 476–484.
- Tardivo J.P. et al. (2005). Methylene blue in photodynamic therapy: From basic mechanisms to clinical applications. Photodiagnosis and Photodynamic Therapy, 2(3), 175–191.
- Logg K. et al. (2009). Faster scrambling of methylene blue in living cells. FEMS Microbiology Letters, 293(1), 127–132.
This tab is a quick safety summary only. A full 16-section GHS SDS (Australian WHS Regulations 2023 / GHS 7th Edition) is available for download.
Section 1 — Identification
| Product Name | Methylene Blue 1% w/v Aqueous Solution |
| Catalogue No. | AS-54001 |
| Intended Use | Laboratory reagent, biological stain, redox indicator — research use only |
| Supplier | AuSaMicS Pty Ltd | 31 Longview CT, Thomastown VIC 3074 | ABN 56 676 640 467 |
| Contact | support@ausamics.com.au · +61 412 520 598 |
| Emergency | Poisons Information Centre: 13 11 26 |
Section 2 — Hazard Identification
| GHS Classification | Acute Toxicity (Oral) Category 4 — Harmful if swallowed |
| Signal Word | WARNING |
| Hazard Statement | H302 — Harmful if swallowed |
| Precautionary Statements | P260 · P264 · P270 · P301+P312 |
First Aid
| Inhalation | Move to fresh air. Seek medical advice if symptoms persist. |
| Skin Contact | Wash with soap and water. Dye will stain skin and clothing. |
| Eye Contact | Rinse with water for several minutes. Seek medical advice if irritation persists. |
| Ingestion | Rinse mouth. Do not induce vomiting. Seek medical advice. |
Handling, Storage & Disposal
- Wear lab coat, nitrile gloves and safety glasses during all handling.
- Use in a well-ventilated laboratory area or fume cupboard.
- Store at 15–25°C, protected from direct light. Keep container closed.
- Dispose according to local laboratory chemical waste procedures and EPA guidelines.
- Do not dispose via sink or drain without appropriate dilution and compliance with trade waste requirements.
Typical specification shown. Lot-specific COA available on request — email support@ausamics.com.au with order number and lot number.
Product Details
| Product Name | Methylene Blue 1% w/v Aqueous Solution |
| Catalogue No. | AS-54001 |
| CAS No. | 61-73-4 |
| Lot No. | Assigned per production batch |
| Manufacture Date | As printed on label |
| Expiry Date | 24 months from manufacture (as printed on label) |
| Storage | 15–25°C, protect from light |
Analytical Results
| Test | Method | Specification | Result |
|---|---|---|---|
| Appearance | Visual | Clear deep blue solution | ✓ Conforms |
| Identity λmax ~664 nm | UV-Vis Spectrophotometry | Conforms to reference | ✓ Conforms |
| Concentration | Formulation control | 1% w/v, 10 mg/mL | ✓ Conforms |
| pH (25°C) | Potentiometric | 3.0 – 4.5 | ✓ Conforms |
| Heavy Metals | ICP / AAS or equivalent | Below standard threshold | ✓ Conforms |
| Particulate Matter | Visual | Absent | ✓ Conforms |
Authorisation
| Tested By | AuSaMicS Quality Control Laboratory, Thomastown VIC |
| Authorised By | Technical Director, AuSaMicS Pty Ltd |
| Release Status | ✓ Released |
For laboratory and research use only. Not for human consumption, therapeutic, veterinary, or household use.
AuSaMicS Pty Ltd · ABN 56 676 640 467 · 31 Longview CT, Thomastown VIC 3074, Australia · support@ausamics.com.au · +61 412 520 598 · ausamics.com.au.
Information is provided in good faith and should be verified against the controlled SDS, TDS and COA for the supplied batch.