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MRS Agar (De Man, Rogosa & Sharpe) | Lactobacillus Culture Medium, Cat: AS-1295
$49.00 AUD
Documentation
AuSaMicS Life Science™ · Dehydrated Culture Media · Food Microbiology · Probiotics · Fermentation
MRS Agar (De Man, Rogosa & Sharpe Medium)
Selective Medium for Lactic Acid Bacteria | Cat: AS-1295
A selective, nutrient-rich microbiological culture medium formulated for the cultivation, isolation and enumeration of Lactobacillus species and other lactic acid bacteria (LAB). Suitable for food microbiology, dairy quality control, probiotic research and fermentation studies.
AS-1295 Dehydrated Powder LAB Medium COA + SDS Available
Quick Specs
Catalogue AS-1295
Format Dehydrated Powder
Application LAB Cultivation
Preparation Heat · Sterilise · Pour
Documentation TDS · SDS · COA
Overview
MRS Agar (de Man, Rogosa and Sharpe Agar) is a selective, nutrient-rich medium developed for the cultivation of lactic acid bacteria, particularly Lactobacillus species.
The formulation provides peptides, amino acids, vitamins, carbohydrates, mineral salts and buffering components that support LAB growth while creating conditions less favourable for many competing microorganisms.
Recommended for LAB work: MRS Agar is widely used in food, dairy, probiotic and fermentation microbiology where recovery and enumeration of lactic acid bacteria are required.
Applications
| Application | Typical Use |
|---|---|
| Lactobacillus cultivation | Isolation, maintenance and enumeration of Lactobacillus species |
| Lactic acid bacteria | Recovery and cultivation of other LAB from food and fermentation samples |
| Food & dairy microbiology | Yoghurt, cheese, fermented foods and related quality-control applications |
| Probiotic research | Research, screening and quality-control work involving probiotic LAB |
| Fermentation studies | Academic and industrial studies involving carbohydrate utilisation and LAB growth |
Key Features
Nutrient rich: provides multiple nitrogen sources, growth factors and fermentable carbohydrate for LAB.
Selective environment: formulation conditions favour lactic acid bacteria over many competing organisms.
Convenient format: supplied as a dehydrated powder for routine laboratory preparation.
Quality documentation: lot-specific COA, SDS and technical documentation are available.
Technical Data Sheet Summary
Product Specifications
| Catalogue Number | AS-1295 |
| Product Name | MRS Agar | De Man, Rogosa & Sharpe Medium |
| Format | Dehydrated microbiological culture medium |
| Appearance | Beige to light yellow powder |
| Application | Cultivation, isolation and enumeration of lactic acid bacteria |
| Storage | Store tightly closed in a cool, dry place, protected from moisture and direct light. |
Typical Preparation
| Step 1 | Suspend the recommended quantity of dehydrated MRS Agar in purified water. |
| Step 2 | Heat with frequent agitation until the medium is completely dissolved. |
| Step 3 | Sterilise by autoclaving at 121°C for 15 minutes, unless otherwise specified on the product label or batch documentation. |
| Step 4 | Cool to approximately 45–50°C before pouring into sterile Petri dishes. |
Preparation note: Follow the concentration and final pH stated on the current AuSaMicS product label, TDS or lot-specific documentation supplied with the product.
Typical Incubation Guidance
| Organisms | Lactobacillus species and other lactic acid bacteria |
| Atmosphere | Aerobic, microaerophilic or anaerobic conditions depending on the organism and method |
| Temperature | Use the incubation temperature specified by the applicable method or target organism |
| Duration | Incubation time depends on strain, sample type and validated laboratory method |
Safety Data Sheet Summary
Laboratory product: Handle using standard laboratory hygiene practices and appropriate personal protective equipment.
| Intended Use | Laboratory, research, educational and approved industrial microbiology use only |
| Human / Veterinary Use | Not intended for human or veterinary therapeutic use |
| Handling | Avoid generating or inhaling dust. Avoid contact with eyes and prolonged skin contact. |
| PPE | Laboratory coat, gloves and eye protection recommended during handling and preparation |
| Storage | Keep container tightly closed in a cool, dry location protected from moisture |
| Biological Safety | Once inoculated, treat prepared medium as potentially biohazardous and follow institutional biosafety procedures |
| Disposal | Dispose of unused product and inoculated media in accordance with local laboratory and biological waste requirements |
Full SDS: Contact support@ausamics.com quoting catalogue number AS-1295 for the current Safety Data Sheet.
Certificate of Analysis
Each released batch of MRS Agar (AS-1295) can be supplied with a lot-specific Certificate of Analysis documenting applicable finished-product quality checks.
| Test Parameter | Typical Specification / Release Check |
|---|---|
| Dehydrated Appearance | Beige to light yellow, homogeneous powder |
| Prepared Appearance | Clear to slightly opalescent, light amber gel |
| pH | Within the product specification stated on the batch COA |
| Growth Promotion | Supports expected growth of appropriate lactic acid bacteria reference strains |
| Physical Performance | Acceptable gel formation and appearance after recommended preparation |
| Batch Documentation | Lot number, manufacture / release data and applicable QC results |
Batch-specific COA: Contact support@ausamics.com quoting the product catalogue number and lot number.