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MRS Broth (De Man, Rogosa & Sharpe) | Lactobacillus Culture Medium | Dehydrated | AS-1296
  • MRS Broth (De Man, Rogosa & Sharpe) | Lactobacillus Culture Medium | Dehydrated | AS-1296
  • MRS Broth (De Man, Rogosa & Sharpe) | Lactobacillus Culture Medium | Dehydrated | AS-1296
MRS Broth for Lactic Acid Bacteria (LAB) | De Man, Rogosa & Sharpe Medium

MRS Broth for Lactic Acid Bacteria (LAB) | De Man, Rogosa & Sharpe Medium

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AuSaMicS Life Science  ·  Microbiology Culture Media

MRS Broth (De Man, Rogosa & Sharpe)

Selective Enrichment Medium for Lactobacillus & Lactic Acid Bacteria

The gold-standard dehydrated liquid medium for cultivation, enrichment, and enumeration of LAB. Probiotics. Dairy. Fermentation. Food safety.

AS-1296 pH 6.2 ± 0.2 55.15 g/L ✓ Oxoid CM0359 Equivalent ✓ BD Difco 288130 Equivalent Aerobic & Anaerobic Use 🇦🇺 Melbourne Stock ⚡ Same-Week Dispatch 📄 COA Every Batch
pH (25°C)
6.2 ± 0.2
Optimal for LAB
Dissolution
55.15 g/L
In distilled water
Sterilisation
121°C
15 min autoclave
Incubation
37°C
24–48 hours
📄 Full DocumentationCOA · TDS · SDS every batch
🇦🇺 Australian StockNo import lead times
⚡ Same-Week DispatchMelbourne warehouse
🔬 Technical SupportFrom our science team
Overview

MRS Broth (De Man, Rogosa & Sharpe) is the internationally recognised gold-standard dehydrated selective enrichment liquid culture medium for the cultivation, enrichment, and enumeration of Lactobacillus species and lactic acid bacteria (LAB). Originally formulated by De Man, Rogosa, and Sharpe in 1960, MRS Broth provides a nutritionally rich, mildly selective environment that supports robust LAB growth under both aerobic and anaerobic conditions — making it indispensable in probiotic research, dairy microbiology, fermentation science, and food safety testing.

AuSaMicS AS-1296 is manufactured and packed in Melbourne to the same specification as Oxoid CM0359 and BD Difco 288130, with same-week dispatch and a batch-specific COA with every order.

Why MRS Broth? — Principle of Selectivity

MRS Broth achieves its selectivity through a combination of nutritional enrichment and mild chemical inhibition:

Sodium Acetate — creates a mildly acidic, acetate-buffered environment that inhibits many competing non-LAB organisms including Gram-negative bacteria and enterococci, while LAB thrive in acetate-containing media.

Ammonium Citrate — inhibits Gram-negative bacteria by chelating divalent cations required for their growth. LAB are relatively resistant.

Tween 80 (Polysorbate 80) — a non-ionic surfactant that stimulates LAB growth by providing fatty acid precursors for membrane biosynthesis. Critical for some Lactobacillus strains that cannot synthesise oleic acid independently.

pH 6.2 — slightly below neutral; optimal for most LAB and mildly restrictive for neutral-preferring competitors.

Organisms Supported — Growth Profile
Organism Growth Application
Lactobacillus acidophilus ATCC 4356 ✓ Good to excellent growth Probiotic research, dairy QC
Lactobacillus casei ATCC 393 ✓ Good to excellent growth Probiotic products, fermented beverages
Lactobacillus plantarum ATCC 14917 ✓ Excellent growth Fermented vegetables, sourdough
Lactobacillus rhamnosus ATCC 7469 ✓ Good growth Probiotic supplements
Lactobacillus bulgaricus ✓ Good growth Yoghurt starter culture QC
Lactobacillus helveticus ✓ Good growth Cheese microbiology
Lactococcus lactis ✓ Good growth Dairy starter culture research
Pediococcus acidilactici ✓ Moderate to good Meat fermentation, beer spoilage
Leuconostoc mesenteroides ✓ Moderate growth Sauerkraut, kimchi fermentation
Escherichia coli ✗ Inhibited Indicator of selectivity performance
Staphylococcus aureus ✗ Inhibited Indicator of selectivity performance
Applications
🧫
Probiotic Research
Cultivation of probiotic strains, viability testing, inoculum preparation
🥛
Dairy Microbiology
Yoghurt, cheese, kefir starter culture QC and enumeration
🍞
Fermented Foods
Sourdough, sauerkraut, kimchi, miso LAB characterisation
🍺
Beverage QC
Beer spoilage LAB detection, wine and cider microbiology
🔬
LAB Enrichment
Enrichment prior to plating; inoculum standardisation
📊
Food Safety
LAB enumeration in processed and fermented food products
MRS Broth vs MRS Agar — Which Do You Need?
Parameter MRS Broth (AS-1296) ★ MRS Agar (AS-1295)
Format Liquid — no agar Solid — 15 g/L agar added
Primary Use Enrichment, inoculum prep, fermentation studies, biomass production Colony isolation, enumeration, morphology characterisation
Growth detection Turbidity (OD measurement) or visual turbidity Colony count per plate
Use together? Yes — MRS Broth for enrichment/growth phase → MRS Agar for plating and colony analysis
Storage & Shelf Life
Powder: Store in a cool, dry location, tightly sealed, protected from moisture and direct light.
Prepared broth: Store at 2–8°C under sterile conditions. Use within 4 weeks. Discard if turbidity, precipitation, or colour change is observed before inoculation.
Shelf life: Stated on label — typically 3–5 years from manufacture under recommended conditions.
Cross-Reference / Equivalent Products
Brand Product Name Cat. No. Notes
Oxoid (Thermo Fisher) MRS Broth CM0359 Imported
BD Difco Lactobacilli MRS Broth 288130 Imported
HiMedia MRS Broth M369 Imported
Merck (Sigma-Aldrich) MRS Broth 1.10660 Imported
AuSaMicS MRS Broth (De Man, Rogosa & Sharpe) AS-1296 Melbourne stock · COA · same-week dispatch
Intent-Based Search Queries
MRS broth Australia Lactobacillus culture medium Melbourne Oxoid CM0359 alternative Australia lactic acid bacteria enrichment broth probiotic research media supplier dairy microbiology media Australia De Man Rogosa Sharpe medium buy fermented food microbiology medium
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Product Identification
Product Name MRS Broth (De Man, Rogosa & Sharpe)
Catalogue Number AS-1296
Synonyms MRS Medium; Lactobacilli MRS Broth; De Man Rogosa Sharpe Broth; LAB Enrichment Broth
Medium Type Selective enrichment liquid culture medium (broth)
Form Dehydrated powder
Original Reference De Man JC, Rogosa M, Sharpe ME. A medium for the cultivation of Lactobacilli. J Appl Bacteriol. 1960;23(1):130–135.
Commercial Equivalents Oxoid CM0359 | BD Difco 288130 | HiMedia M369 | Merck 1.10660
HS / AHECC Code 3821.00.00 — Prepared culture media for development of microorganisms
Manufacturer AuSaMicS Pty Ltd, Melbourne, Australia
Composition (per litre of prepared medium)
Ingredient g/L CAS Function
Peptone (Proteose Peptone) 10.0 73049-73-7 Primary nitrogen and amino acid source; supports protein biosynthesis in LAB
Beef Extract (Meat Extract) 10.0 8038-63-9 Provides additional nitrogen, vitamins, and carbon sources; supports growth factors
Yeast Extract 5.0 8013-01-2 B-complex vitamins, nucleotides, and accessory growth factors; essential for LAB
Glucose (D-Glucose) 20.0 50-99-7 Primary fermentable carbohydrate; catabolised via homo- or heterofermentative pathways to lactic acid
Tween 80 (Polysorbate 80) 1.0 9005-65-6 Non-ionic surfactant; provides fatty acid precursors for LAB membrane biosynthesis; stimulates growth of oleic acid-requiring strains
Dipotassium Hydrogen Phosphate (K₂HPO₄) 2.0 7758-11-4 Phosphate buffer component; maintains pH stability during lactic acid production
Sodium Acetate 5.0 127-09-3 Selective inhibitor of competing non-LAB flora; acetate buffer component at pH 6.2
Triammonium Citrate (Ammonium Citrate) 2.0 3458-72-8 Selectively inhibits Gram-negative bacteria by chelating divalent metal ions; LAB-permissive
Magnesium Sulfate (MgSO₄·7H₂O) 0.2 10034-99-8 Essential cofactor for numerous LAB enzymes including ATPases and glycolytic enzymes
Manganese Sulfate (MnSO₄·H₂O) 0.05 10034-96-5 Essential trace element; cofactor for superoxide dismutase and other LAB-specific enzymes
Total: 55.15 g/L  |  pH 6.2 ± 0.2 at 25°C  |  Autoclave 121°C / 15 min
Physicochemical Specifications
Parameter Specification Method
Appearance (powder) Light yellow to beige, free-flowing powder Visual
Appearance (prepared broth) Clear to slightly opalescent, light amber solution Visual after dissolution
pH (prepared, 25°C) 6.2 ± 0.2 Potentiometric (calibrated pH meter)
Moisture content ≤ 5.0% Loss on drying, 105°C
Dissolution Complete at 55.15 g/L with gentle heating Visual inspection
Storage (powder) Tightly sealed, cool and dry, away from moisture and light —
Storage (prepared broth) 2–8°C, sterile, up to 4 weeks —
Preparation Protocol
1
Suspend 55.15 g of dehydrated MRS Broth (AS-1296) in 1 litre of distilled or deionised water.
2
Mix thoroughly. Heat gently with continuous agitation until the powder is completely dissolved. The broth should appear clear to slightly opalescent and light amber.
3
Dispense into tubes or flasks. Autoclave at 121°C for 15 minutes. Do not overheat — excessive heat darkens the broth and may reduce growth performance.
4
Cool to room temperature before inoculation. Inoculate and incubate at 37°C for 24–48 hours. For microaerophilic or anaerobic strains, use anaerobic incubation conditions or 5% CO₂ atmosphere.
5
Growth is indicated by turbidity. For quantitative work, measure optical density at 600 nm (OD₆₀₀). Discard if turbidity is observed in uninoculated control tubes.
💡 Tip for anaerobic/microaerophilic strains: Fill tubes to capacity and seal, or use an anaerobic jar or GasPak system. Some probiotic Lactobacillus strains show significantly better growth and faster doubling times under reduced oxygen conditions. A 5% CO₂ atmosphere is sufficient for most dairy LAB.
Quality Control Performance
Test Organism ATCC Strain Result (24–48h, 37°C)
Lactobacillus acidophilus ATCC 4356 Good to excellent growth — turbidity confirmed
Lactobacillus casei ATCC 393 Good to excellent growth — turbidity confirmed
Lactobacillus plantarum ATCC 14917 Excellent growth — strong turbidity
Escherichia coli ATCC 25922 Inhibited or markedly reduced growth
Staphylococcus aureus ATCC 25923 Inhibited or markedly reduced growth
Mode of Action

MRS Broth creates a nutritionally enriched, mildly selective environment through five synergistic mechanisms:

# Mechanism Effect
1 Sodium acetate + pH 6.2 Inhibits many Gram-negative and spore-forming organisms; favours acid-tolerant LAB
2 Ammonium citrate Chelates Mg²⁺ and Mn²⁺ from Gram-negative cell envelopes; LAB are resistant
3 Tween 80 Provides oleic acid for LAB membrane biosynthesis; stimulates growth of oleic acid-requiring strains
4 High glucose (20 g/L) Drives rapid homo- or heterofermentative lactic acid production; further acidifies medium and suppresses competitors
5 K₂HPO₄ buffer Maintains pH stability during fermentation; prevents overshoot inhibition of LAB by their own lactic acid
Literature References

1. De Man JC, Rogosa M, Sharpe ME. A medium for the cultivation of Lactobacilli. Journal of Applied Bacteriology. 1960;23(1):130–135. [Original paper — the defining reference for this medium]

2. Harrigan WF, McCance ME. Laboratory Methods in Food and Dairy Microbiology. Academic Press; 1976.

3. Kandler O, Weiss N. Regular, nonsporing Gram-positive rods. In: Sneath PHA, et al., eds. Bergey's Manual of Systematic Bacteriology. Vol. 2. Williams & Wilkins; 1986:1208–1234.

4. ISO 11133:2014. Culture media — Preparation, production, storage and performance testing. ISO, Geneva.

5. ISO 19344:2015. Milk and milk products — Starter cultures, probiotics and ferments — Quantification of lactic acid bacteria. ISO, Geneva.

Download TDS (PDF)
📄 Full 16-section GHS SDS available — Australian WHS Regulations 2023 / GHS 7th Edition. Download below or request via support@ausamics.com.au
Section 1 — Identification
Product Name MRS Broth (De Man, Rogosa & Sharpe)
Catalogue No. AS-1296
Supplier AuSaMicS Pty Ltd | ABN 56 676 640 467
Address 31 Longview CT, Thomastown VIC 3074, Australia
Phone / Email +61 412 520 598 | support@ausamics.com.au
Emergency Poisons Information Centre: 13 11 26 (Australia, 24 hr)
Intended Use Selective enrichment liquid culture medium for cultivation of lactic acid bacteria. For laboratory and research use only.
Section 2 — Hazard Identification
GHS Classification NOT classified as a hazardous substance under Australian WHS Regulations 2023 / GHS 7th Edition at intended use concentrations.
Signal Word None required
Hazard Pictograms None applicable
Other Hazards Combustible dry powder — avoid ignition sources in bulk handling. Tween 80 component: mild skin/eye irritant in concentrated form at use level in this formulation.
Section 3 — Composition
Component CAS g/L GHS Classification
Peptone (Proteose) 73049-73-7 10.0 Not classified
Beef / Meat Extract 8038-63-9 10.0 Not classified
Yeast Extract 8013-01-2 5.0 Not classified
Glucose (D-Glucose) 50-99-7 20.0 Not classified
Tween 80 (Polysorbate 80) 9005-65-6 1.0 Mild irritant at high concentration — not classified at use level
Dipotassium Hydrogen Phosphate 7758-11-4 2.0 Not classified
Sodium Acetate 127-09-3 5.0 Not classified at use level
Ammonium Citrate 3458-72-8 2.0 Not classified at use level
Magnesium Sulfate 10034-99-8 0.2 Not classified
Manganese Sulfate 10034-96-5 0.05 Not classified at use level
Sections 4–16 Summary
Section Summary
4. First Aid Inhalation: fresh air. Skin/eye: wash with water 15 min. Ingestion: rinse mouth, water; seek advice. No specific antidote.
5. Fire Fighting Not flammable (powder combustible in bulk). CO2, dry chemical, foam, water spray. Combustion: CO2, CO, NOx.
6. Accidental Release Powder: avoid dust; sweep/vacuum; sealed container. Prepared broth: absorb with inert material; dispose per EPA. Avoid release to drains (BOD loading).
7. Handling & Storage Well-ventilated area. Avoid dust inhalation. Store cool, dry, tightly sealed. Prepared broth: 2–8°C, use within 4 weeks. Keep from oxidising agents.
8. Exposure Controls / PPE P1 dust mask (bulk powder). Nitrile gloves. Safety spectacles. Lab coat. OEL: general inhalable dust limit (10 mg/m3). LEV recommended for bulk handling.
9. Physical & Chemical Powder: light yellow-beige. Broth: amber, opalescent. pH 6.2 ± 0.2. Not flammable, not explosive, not oxidising.
10. Stability & Reactivity Stable under recommended conditions. Avoid strong oxidising agents, strong acids/bases. Glucose component may caramelise on prolonged overheating.
11. Toxicological Info Low acute toxicity at intended use concentrations. Tween 80: mild skin/mucous irritant at high concentrations. No carcinogenicity/mutagenicity evidence.
12. Ecological Info Biodegradable organic matrix. High glucose content contributes to BOD — avoid bulk discharge to natural waterways. Low acute aquatic toxicity.
13. Disposal Uninoculated: non-hazardous organic waste per local EPA. Inoculated broth: autoclave 121°C/15 min before disposal as microbiological waste.
14. Transport Not classified as dangerous goods — ADG, IATA, IMDG. No UN Number. No special transport requirements.
15. Regulatory Australian WHS Regulations 2023 / GHS 7th Edition. AICIS compliant. HS 3821.00.00. For laboratory use only — not a food ingredient.
16. Other Info SDS-AS-1296-MRS | Issue: January 2026 | Review: January 2028 | Prepared by Hassan Salimi, AuSaMicS Pty Ltd.
⚠️ Biosafety Note: MRS Broth is a non-selective enrichment broth when used with unknown clinical or environmental samples. Lactobacillus species from clinical specimens should be handled under BSL-2 precautions until identification is complete. For ATCC reference strains, BSL-1 precautions are appropriate.
Download Full SDS (PDF)
Certificate of Analysis — AS-1296

Each batch of MRS Broth (AS-1296) is manufactured under quality-controlled conditions and released with a batch-specific Certificate of Analysis confirming compliance with all physicochemical and microbiological performance specifications.

Analytical Results — Typical Specifications
# Test Parameter Specification Method Result
1 Appearance (powder) Light yellow to beige, free-flowing Visual Conforms
2 Appearance (prepared broth) Clear to slightly opalescent, light amber Visual Conforms
3 pH (prepared, 25°C) 6.2 ± 0.2 Potentiometric Conforms
4 Moisture Content ≤ 5.0% Loss on drying, 105°C Conforms
5 Dissolution Complete at 55.15 g/L with gentle heating Visual inspection Conforms
6 Lactobacillus acidophilus ATCC 4356 (positive control) Good to excellent growth within 48h at 37°C Turbidity / OD600 Conforms
7 Lactobacillus casei ATCC 393 (positive control) Good to excellent growth within 48h at 37°C Turbidity / OD600 Conforms
8 Lactobacillus plantarum ATCC 14917 (positive control) Excellent growth within 24–48h at 37°C Turbidity / OD600 Conforms
9 Escherichia coli ATCC 25922 (negative control) Inhibited or markedly reduced growth Turbidity Conforms
10 Staphylococcus aureus ATCC 25923 (negative control) Inhibited or markedly reduced growth Turbidity Conforms
CONCLUSION: BATCH CONFORMS TO ALL STATED SPECIFICATIONS — RELEASED FOR SUPPLY
Batch Traceability
📋 Batch Details — Stated on Label and Supplied COA
Product
MRS Broth (De Man, Rogosa & Sharpe)
Catalogue No.
AS-1296
Medium Type
Selective enrichment liquid culture medium
pH (prepared)
6.2 ± 0.2 at 25°C
Batch Number
Stated on label
Manufacturing Date
Stated on label
Expiry Date
Stated on label (3–5 years)
Storage
Cool, dry, tightly sealed
Manufacturer
AuSaMicS Pty Ltd — Melbourne, Australia
COA Status
Included with every order
Frequently Asked Questions
❓ Which Lactobacillus strains grow in MRS Broth?
MRS Broth supports most Lactobacillus species including L. acidophilus, L. casei, L. plantarum, L. rhamnosus, L. bulgaricus, L. helveticus, L. delbrueckii, and many others. It also supports Lactococcus lactis, Pediococcus spp., and Leuconostoc spp. The selective components (sodium acetate, ammonium citrate) suppress most competing Gram-negative flora while permitting unrestricted LAB growth.
❓ What is the difference between MRS Broth and MRS Agar?
MRS Broth (AS-1296) is a liquid medium used for enrichment, inoculum preparation, fermentation studies, and turbidimetric growth measurement. MRS Agar (AS-1295) contains 15 g/L agar and is used for colony isolation, enumeration by colony count, and morphology characterisation. The two are frequently used together — MRS Broth for the liquid growth phase, MRS Agar for plating and analysis.
❓ Aerobic or anaerobic incubation?
MRS Broth supports LAB growth under both conditions. For best results with most Lactobacillus strains: 37°C, 24–48 hours, aerobic. For strict microaerophiles or anaerobes (e.g. some probiotic strains): use anaerobic jar with GasPak sachet or 5% CO₂ atmosphere for significantly improved growth yield and faster doubling times.
❓ Is AS-1296 equivalent to Oxoid CM0359?
Yes — AS-1296 follows the same De Man, Rogosa & Sharpe formulation with identical pH (6.2 ± 0.2) and dissolution (55.15 g/L), meeting the same LAB cultivation performance standard. Equivalent to Oxoid CM0359, BD Difco 288130, HiMedia M369, and Merck 1.10660. Manufactured and packed in Melbourne with same-week dispatch and a batch-specific COA.
Packaging
Standard Pack Sizes 100 g  ·  250 g  ·  500 g  ·  1 kg
Bulk / Custom Available — contact support@ausamics.com.au
AuSaMicS Pty Ltd — Quality Assurance
✓ De Man, Rogosa & Sharpe formulation — published reference specification
✓ QC performance testing: 3 positive and 2 negative control organisms per batch
✓ ISO 11133:2014 compliant quality management
✓ COA, TDS, and SDS with every order
✓ Australian stock — same-week dispatch, no import lead times
⚠️ For laboratory, food microbiology, fermentation research, and educational use only. Not for diagnostic, medical, therapeutic, food preparation, or veterinary use. AuSaMicS Pty Ltd accepts no liability for use outside the intended application.
Download COA (PDF) Request Batch COA

 

AuSaMicS Pty Ltd  |  31 Longview CT, Thomastown VIC 3074, Australia  |  ABN 56 676 640 467
support@ausamics.com.au  |  +61 412 520 598  |  www.ausamics.com.au
Information accurate to best of knowledge at time of publication. Specifications subject to change without notice. AuSaMicS Pty Ltd accepts no liability for use outside the intended laboratory application.

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