MRS Broth for Lactic Acid Bacteria (LAB) | De Man, Rogosa & Sharpe Medium

MRS Broth (De Man, Rogosa & Sharpe) | Lactobacillus Culture Medium | Dehydrated | AS-1296

De Man, Rogosa & Sharpe Medium for Lactic Acid Bacteria

Product Identification

Chemical & Physical Information

Product Name: MRS Broth (De Man, Rogosa & Sharpe)
Catalogue Number: AS-1296
Medium Type: Selective enrichment liquid culture medium
Form: Dehydrated powder

Product Description

MRS Broth is a nutritionally rich liquid culture medium specifically formulated for the cultivation, enrichment, and enumeration of Lactobacillus species and other lactic acid bacteria (LAB). Developed by De Man, Rogosa, and Sharpe, this medium supports robust growth of LAB under both aerobic and anaerobic conditions.

The carefully balanced formulation provides essential carbon sources, nitrogen, vitamins, salts, and buffering agents required for optimal metabolic activity and acid tolerance of lactic acid bacteria. MRS Broth is widely used in probiotic research, food and dairy microbiology, fermentation studies, and quality control testing.

Ausamics MRS Broth is supplied as a high-quality dehydrated powder to ensure consistent solubility, reproducible performance, and reliable batch-to-batch quality.

Principle of the Medium

Functional Components

Peptone and Meat Extract: Supply amino acids, peptides, and nitrogen sources
Yeast Extract: Provides B-complex vitamins and growth factors
Glucose: Primary fermentable carbohydrate for LAB metabolism
Sodium Acetate and Ammonium Citrate: Inhibit competing flora
Magnesium and Manganese Salts: Support enzymatic activity
Buffering System: Maintains stable pH during acid production

Typical Characteristics

Medium Properties

Appearance (dehydrated): Light yellow to beige powder
Appearance (prepared): Clear to slightly opalescent solution
pH (prepared medium): Approximately 6.2 ± 0.2 at 25 °C
Selectivity: Favourable for lactic acid bacteria
Physical State: Liquid after preparation

Typical Applications

Probiotic & Lactic Acid Bacteria Research

Cultivation and enumeration of Lactobacillus spp.
Growth of lactic acid bacteria for probiotic studies

Food, Dairy & Beverage Microbiology

Fermentation studies
Quality control testing of fermented foods and beverages

Research & Quality Control Laboratories

Microbiological research
Routine laboratory analysis and method development

Preparation Instructions

Medium Preparation

Suspend the required quantity of dehydrated medium in demineralised water
Heat gently with agitation until fully dissolved
Autoclave at 121 °C for 15 minutes
Cool to room temperature before use

Storage & Stability

Storage Conditions

Store dehydrated medium in a cool, dry place
Keep container tightly closed and protected from moisture
Prepared medium may be stored at 2–8 °C under sterile conditions
Do not use if contamination, precipitation, or abnormal turbidity is observed

Intended Use

Regulatory Statement

For laboratory research, food microbiology, fermentation studies, and educational use only.

Not intended for diagnostic, medical, food preparation, or veterinary applications.

Quality & Supply Assurance

Manufacturing & Documentation

Manufactured under controlled conditions
Consistent batch-to-batch performance
Certificate of Analysis (COA) available upon request
Safety Data Sheet (SDS) available
Technical support provided by Ausamics Life Science

Customs & Trade Information

Classification

HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms