Skip to product information
Request a Quote
Nutrient Agar with MUG | AS-1308 | Rapid E. coli Detection | AuSaMicS
$65.00 AUD
Complete Formulation & Fluorogenic Mechanism
📋 Ingredient Composition (per Liter)
💡 Fluorogenic Detection Mechanism
E. coli β-glucuronidase enzyme
↓
MUG → 4-Methylumbelliferone + Glucuronic acid
↓
Bright blue-white fluorescence (366nm UV)
↓
MUG → 4-Methylumbelliferone + Glucuronic acid
↓
Bright blue-white fluorescence (366nm UV)
Critical: >97% of E. coli strains are GUD positive (Exception: E. coli O157:H7 typically GUD negative)
📊 Complete E. coli Detection Media Portfolio
Strategic Method Selection Guide
✅ Nutrient Agar + MUG Advantages
- ✓ Maximum Recovery: 95-99% for stressed cells
- ✓ Non-Selective Base: No inhibition of injured cells
- ✓ Rapid Detection: Results in 4-24 hours
- ✓ Cost Effective: Simple, economical formulation
- ✓ Heat-Stressed Cells: Ideal for processed foods
- ✓ Universal Application: Food, water, clinical
⚠️ Method Considerations
- ▲ Background Growth: Non-coliforms also grow
- ▲ UV Equipment Required: 366nm longwave lamp
- ▲ O157:H7 Exception: Typically GUD negative
- ▲ Confirmation Needed: Presumptive test only
- ▲ Light Sensitivity: MUG degrades over time
🎯 When to Choose Each Method
- ◆ Nutrient+MUG: Heat-treated/processed foods
- ◆ TBX: Raw foods, direct enumeration
- ◆ MacConkey+MUG: Mixed flora samples
- ◆ Chromocult: Enhanced specificity needed
- ◆ EC+MUG: Thermotolerant confirmation
📊 Sample-Specific Performance Matrix
🔬 UV Detection Protocol & Quality Control
UV Lamp Specs:
366nm longwave
15W minimum output
366nm longwave
15W minimum output
Reading Conditions:
Dark room environment
6-12 inch distance
Dark room environment
6-12 inch distance
Positive Control:
E. coli ATCC 25922
Bright blue fluorescence
E. coli ATCC 25922
Bright blue fluorescence
Negative Control:
Enterobacter aerogenes
No fluorescence
Enterobacter aerogenes
No fluorescence
📋 Technical Specifications
| Catalogue Number | AS-1308 |
| Base Weight | 28.0 g/L (with MUG) |
| Final pH | 7.0 ± 0.2 (at 25°C) |
| MUG Concentration | 100 mg/L |
| Appearance (Powder) | Cream to light tan |
| Prepared Medium | Clear, light amber |
| Sterilization | 121°C, 15 minutes |
| Incubation | 35-37°C, 18-24 hours |
| Shelf Life | 3 years |
Fluorescent Detection
- Excitation: 366nm UV longwave
- Emission: 445nm blue-white
- Intensity: Brilliant fluorescence
- Detection Time: 4-24 hours
- Specificity: >97% E. coli positive
Critical Success Factors
- Temperature: Add MUG after cooling to 50°C
- Light Protection: Store plates in darkness
- UV Lamp: 366nm longwave essential
- Timing: Read at 4-6h and 18-24h🔍 Confirmation: Consider O157:H7 testing
Regulatory Standards
- ✓ FDA BAM Chapter 4A - E. coli detection
- ✓ ISO 16649-3:2015 - MUG method
- ✓ USDA-FSIS MLG 5B - Meat products
- ✓ APHA Standard Methods - Water testing
- ✓ Health Canada MFHPB-27 - Food methods
✅ Quality Standards
- ✓ Performance Testing: Reference strains
- ✓ MUG Potency: Fluorescence verification
- ✓ pH Verification: 7.0 ± 0.2 confirmed
- ✓ Sterility Testing: USP <71> compliant
- ✓ Stability Studies: 36 months validated
Complete E. coli Detection System
E. coli Detection Media
Supporting Reagents & Equipment
Confirmation & Identification
Need E. coli Detection Support?
Our food safety microbiologists can help optimize your fluorogenic detection protocols
For food safety testing and laboratory use only. Not for food, feed, household, cosmetic, therapeutic, or personal use.
AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
Same-day dispatch • Australian stock • FDA-BAM compliance documentation included