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Nutrient Broth | General Purpose Liquid Culture Medium l AS-1309
$85.00 AUD
Documentation
Nutrient Broth
General-Purpose Liquid Culture Medium | Cat. No. AS-1309
Cat. No. AS-1309 pH 7.2 ± 0.2 13.0 g/L Non-selective ISO 11133 Compliant 🇦🇺 Made in Melbourne ⚡ Same-Week Dispatch
Overview & Biochemistry
Nutrient Broth is one of the oldest and most widely used general-purpose liquid culture media in microbiology. Its simple, balanced formulation — peptone, yeast extract, and sodium chloride — supports reliable growth of a broad range of non-fastidious bacteria and is the standard liquid medium for routine cultivation, enrichment, inoculum preparation, and purity verification across teaching, research, and quality-control laboratories.
It is the liquid counterpart of Nutrient Agar (AS-1307), sharing the same nutritional base. When a solid medium is not required — for example, for preparing liquid inocula, overnight enrichment, or turbidity-based growth assays — Nutrient Broth is the standard choice.
Mode of Action
Peptone → Amino Acid Supply | Yeast Extract → Vitamins & Co-factors | NaCl → Osmotic Balance
Peptone (5.0 g/L) — a proteolytic digest of animal tissue — provides the primary nitrogen source as readily absorbable peptides and amino acids. Yeast extract (3.0 g/L) contributes B-complex vitamins (B1, B2, B6, B12), folic acid, and nucleotide precursors that are essential co-factors for bacterial metabolism, particularly for organisms that cannot synthesise these de novo. Sodium chloride (5.0 g/L) maintains the osmotic pressure of the medium at physiological levels (~0.9% NaCl equivalent), compatible with the growth of most clinically, industrially, and environmentally relevant bacterial species.
Peptone (5.0 g/L) — a proteolytic digest of animal tissue — provides the primary nitrogen source as readily absorbable peptides and amino acids. Yeast extract (3.0 g/L) contributes B-complex vitamins (B1, B2, B6, B12), folic acid, and nucleotide precursors that are essential co-factors for bacterial metabolism, particularly for organisms that cannot synthesise these de novo. Sodium chloride (5.0 g/L) maintains the osmotic pressure of the medium at physiological levels (~0.9% NaCl equivalent), compatible with the growth of most clinically, industrially, and environmentally relevant bacterial species.
Applications
🧪
Bacterial Cultivation
Non-fastidious species
📈
Enrichment Culture
Pre-plating enrichment
🧬
Inoculum Prep
Standardised suspensions
📊
Purity Checks
Pre-assay verification
🔬
Research
Growth kinetics & assays
🏫
Education
Teaching lab standard
Nutrient Broth vs Related General-Purpose Media
| Medium | Type | Key Difference | Best Use |
|---|---|---|---|
| Nutrient Broth (AS-1309) | Liquid, non-selective | Simple base; no glucose or blood | Routine cultivation, enrichment, inocula |
| Nutrient Agar (AS-1307) | Solid, non-selective | Same formula + 15 g/L agar | Colony isolation, plate counts |
| Tryptic Soy Broth (AS-1371) | Liquid, non-selective | Dual peptone + dextrose; richer | Fastidious organisms, sterility testing |
| Brain Heart Infusion Broth | Liquid, non-selective | Brain/heart infusion; very rich | Highly fastidious organisms |
| R2A Broth (AS-1340) | Liquid, low-nutrient | Very low organic load | Oligotrophic aquatic bacteria |
Cross-Reference / Equivalent Products
| Manufacturer | Product Name | Cat. No. | Notes |
|---|---|---|---|
| Oxoid (Thermo Fisher) | Nutrient Broth No. 2 | CM0001 | Equivalent formulation |
| BD Difco | Nutrient Broth | 234000 | Equivalent formulation |
| Merck Millipore | Nutrient Broth | 1.05443 | Equivalent formulation |
| HiMedia | Nutrient Broth | M002 | Equivalent formulation |
Cross-references are for convenience only. AuSaMicS AS-1309 is manufactured locally in Melbourne — same-week dispatch, no import delays.
Related Products
Product Identification
| Product Name | Nutrient Broth |
| Catalogue Number | AS-1309 |
| Medium Type | Non-selective general-purpose liquid culture medium |
| Solid Counterpart | Nutrient Agar (AS-1307) |
| Dissolution | 13.0 g/L in distilled or deionised water |
| Final pH at 25°C | 7.2 ± 0.2 |
| Sterilisation | Autoclave 121°C for 15 minutes |
| Prepared Broth Appearance | Clear to slightly opalescent, light amber |
| Dehydrated Appearance | Cream to light beige free-flowing powder |
| HS Tariff Code | 3821.00.00 — Prepared culture media for development of microorganisms |
Formula — Per Litre
| Ingredient | g / L | Role |
|---|---|---|
| Peptone | 5.0 g | Primary nitrogen source — peptides and amino acids |
| Yeast Extract | 3.0 g | B-vitamins, nucleotide precursors, accessory growth factors |
| Sodium Chloride (NaCl) | 5.0 g | Osmotic balance — physiological ionic strength |
| Total | 13.0 g/L | Final pH 7.2 ± 0.2 at 25°C |
Preparation Protocol
| 1 | Suspend 13.0 g of dehydrated Nutrient Broth (AS-1309) in 1 litre of distilled or deionised water. |
| 2 | Mix thoroughly until completely dissolved. The prepared broth should appear clear to slightly opalescent, light amber. |
| 3 | Dispense into tubes, bottles, or flasks as required. |
| 4 | Autoclave at 121°C for 15 minutes. |
| 5 | Allow to cool to room temperature before inoculation. Store prepared broth at 2–8°C; use within 4 weeks. |
Quality Control — Typical Organisms
| Organism (ATCC) | Inoculum (CFU) | Incubation | Recovery |
|---|---|---|---|
| Escherichia coli (25922) | ≤100 | 35±2°C / 18–24h | Good |
| Staphylococcus aureus (25923) | ≤100 | 35±2°C / 18–24h | Good |
| Bacillus subtilis (6633) | ≤100 | 35±2°C / 18–24h | Good |
| Pseudomonas aeruginosa (27853) | ≤100 | 35±2°C / 18–24h | Good |
Storage & Stability
| Dehydrated Powder | 15–30°C in tightly sealed container, away from moisture and direct sunlight |
| Prepared Broth | 2–8°C; use within 4 weeks. Discard if turbid prior to inoculation. |
| Shelf Life | Refer to expiry date on label |
Applicable Standards
| ISO 11133:2014 | Culture media performance testing — general purpose media |
| ISO 7218:2007 | Microbiology of food — general requirements for microbiological examination |
| AS/NZS 4276 series | Water microbiology — inoculum preparation reference medium |
Literature & References
| # | Reference |
|---|---|
| 1 | BD Difco & BBL Manual, 2nd ed. Becton Dickinson; 2009. Nutrient Broth, Cat. 234000. [Reference standard composition] |
| 2 | ISO 11133:2014. Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media. Geneva: ISO; 2014. |
| 3 | Murray PR, et al. Manual of Clinical Microbiology. 9th ed. ASM Press; 2007. [Nutrient Broth as standard general-purpose medium] |
| 4 | Oxoid. Product Technical Information: Nutrient Broth No. 2 (CM0001). Thermo Fisher Scientific; 2023. |
📄 Full SDS available: Download the complete 16-section GHS Safety Data Sheet (Australian WHS Regulations 2023 / GHS 7th Edition) — contact support@ausamics.com.au
Section 1 — Identification
| Product Name | Nutrient Broth |
| Catalogue No. | AS-1309 |
| Supplier | AuSaMicS Pty Ltd | ABN 56 676 640 467 |
| Address | 31 Longview CT, Thomastown VIC 3074, Australia |
| Emergency | Poisons Information Centre: 13 11 26 (24 hr, Australia) |
| Phone | +61 412 520 598 |
| support@ausamics.com.au |
Section 2 — Hazard Identification (GHS 7th Ed / WHS 2023)
| GHS Classification | NOT classified as a hazardous substance or dangerous goods under Australian WHS Regulations 2023 / GHS 7th Edition. |
| Signal Word | None required |
| Hazard Statements | None required |
| Other Hazards | Fine powder is combustible — avoid dust accumulation near ignition sources. No PBT or vPvB components at ≥0.1%. |
Section 3 — Composition
| Component | g/L | CAS | Hazard |
|---|---|---|---|
| Peptone | 5.0 | 73049-73-7 | Not hazardous |
| Yeast Extract | 3.0 | 8013-01-2 | Not hazardous |
| Sodium Chloride | 5.0 | 7647-14-5 | Not hazardous |
Sections 4–8 — First Aid, Fire & PPE
| Inhalation | Remove to fresh air. Seek medical attention if symptoms persist. |
| Skin Contact | Wash with soap and water ≥15 min. No significant hazard expected. |
| Eye Contact | Irrigate with water ≥15 min. Seek medical attention if irritation persists. |
| Ingestion | Rinse mouth. Not expected to be acutely toxic. Call Poisons Centre 13 11 26 if large amounts ingested. |
| Extinguishing Media | Water spray, foam, dry powder, CO₂ |
| Respiratory Protection | P1 particulate filter when weighing bulk powder |
| Eye Protection | Safety glasses recommended during preparation |
| Skin Protection | Nitrile gloves, lab coat when handling cultures |
Sections 13–15 — Disposal, Transport & Regulatory
| Waste Disposal | Autoclave all inoculated broth (121°C / 15 min) before disposal per institutional biosafety procedures and local EPA regulations. |
| Transport | Not classified as dangerous goods — ADG Code, IMDG, IATA |
| Regulatory Framework | Australian WHS Regulations 2023 | GHS 7th Edition | Safe Work Australia COP 2021 |
| AICIS Status | All components listed on Australian Inventory of Industrial Chemicals (AIIC) |
| Poisons Schedule | Not scheduled under SUSMP |
Quality Specifications
| Parameter | Specification | Method |
|---|---|---|
| Appearance (powder) | Cream to light beige, free-flowing, homogeneous | Visual |
| Appearance (prepared) | Clear to slightly opalescent, light amber broth | Visual after dissolution |
| pH (prepared, 25°C) | 7.2 ± 0.2 | pH meter (calibrated) |
| Dissolution | Complete at 13.0 g/L in distilled water | Visual inspection |
| Moisture Content | ≤5.0% | Loss on drying |
| Growth Promotion | Good growth of E. coli ATCC 25922 at ≤100 CFU / 18–24h / 35±2°C | Inoculation test per ISO 11133 |
| Batch COA | Available for every production lot | On request or download |
Manufacturing & Documentation
Manufactured by AuSaMicS Pty Ltd
31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467
✓ Batch-level quality control on every lot (ISO 11133:2014)
✓ Full GHS-compliant documentation (COA, TDS, SDS)
✓ Australian stock — no import delays, no customs holdups
✓ Technical support direct from our microbiology team
31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467
✓ Batch-level quality control on every lot (ISO 11133:2014)
✓ Full GHS-compliant documentation (COA, TDS, SDS)
✓ Australian stock — no import delays, no customs holdups
✓ Technical support direct from our microbiology team
Available Documentation
| 📄 Certificate of Analysis (COA) | Batch-specific | Available on request |
| 📋 Technical Data Sheet (TDS) | Full specifications and preparation notes |
| ⚠️ Safety Data Sheet (SDS) | GHS 7th Ed | WHS 2023 compliant | 16 sections |
⚠️ Intended Use: For laboratory and research use only. Not for medical, food, veterinary, diagnostic, or therapeutic applications.