OF Basal Medium (AS-1310) | Carbohydrate-Free Hugh-Leifson Base

$35.00 AUD

AuSaMicS Life Science • Versatile Base Media

OF Basal Medium

Carbohydrate-Free Hugh & Leifson Base

A customizable semi-solid foundation for metabolic profiling. Supplement with specific carbohydrates to differentiate Gram-negative bacilli based on their ability to oxidize or ferment diverse sugars.

AS-1310 🧪 Modular Formula ✓ Custom Carbohydrates ⚗️ High Precision

Base: Carbohydrate-Free pH Indicator: Bromothymol Blue Form: Semi-Solid

System Utility

Universal Compatibility - Supports all 1% sugar additions

Sensitive Detection - Low peptone prevents alkaline masking

Motility Observation - Semi-solid agar at 0.2-0.3%

Long Shelf Life - 3 years dehydrated

🔬 Research & Diagnostic Use

Custom Sugar Panels

Ideal for creating custom diagnostic panels for Vibrio, Aeromonas, and non-fermenting bacilli.

Reference Labs

Allows standardized basal comparison across different carbohydrate metabolic pathways.

🧪 Nutrient Formulation (per Liter)

Ingredient	Concentration	Function
Casein Peptone	2.0 g	Nitrogen Source (Minimized)
Sodium Chloride	5.0 g	Osmotic Balance
Dipotassium Phosphate	0.3 g	pH Buffering Agent
Bromothymol Blue	0.08 g	Visual pH Indicator
Agar	2.0 g	Semi-solid Matrix

📋 Technical Specs

Cat Number	AS-1310
Final pH	6.8 ± 0.2
Prepared Color	Green
Yield	9.4 g / L

⚗️ Laboratory Prep

1 Suspend 9.4g in 1L purified water.
2 Heat to boiling to dissolve. Autoclave at 121°C for 15 min.
3 Aseptically add 1% sterile carbohydrate solution (post-autoclave).
4 Dispense into tubes; allow to solidify vertically.