OF Glucose Medium (Hugh & Leifson’s Oxidative-Fermentative Test)

$79.00 AUD

AuSaMicS Life Science • Metabolic Differentiation

OF Glucose Medium

Hugh & Leifson’s Oxidative-Fermentative Test

A specialized semi-solid medium used to determine the metabolic pathway of glucose utilization. Essential for identifying Pseudomonas, Acinetobacter, and Enterobacteriaceae.

AS-1311 🧪 Dual-Tube Method ✓ Clinical Microbiology 🧬 Semi-Solid

Indicator: Bromothymol Blue Carbohydrate: Glucose (1.0%) pH: 6.8 ± 0.2

Diagnostic Indicators

Yellow (Both Tubes)
Fermentative Utilization

Yellow (Open Tube Only)
Oxidative Utilization

Green/Blue
Asaccharolytic (No utilization)

🔬 Application Highlights

Systematic Identification

Key for separating oxidative Pseudomonas from fermentative Enterobacteriaceae.

Low-Acid Detection

High carbohydrate-to-peptone ratio ensures even weak acid production is detected.

🧪 Formula Composition

Ingredient	Concentration	Function
Casein Peptone	2.0 g	Nitrogen & Vitamins
D-Glucose	10.0 g	Metabolic Substrate
Bromothymol Blue	0.08 g	pH Indicator (Green to Yellow)
Agar	2.0 - 3.0 g	Semi-solidifying Agent

📋 Technical Specs

Cat Number	AS-1311
Yield	20.1 g / L
Storage	2 - 25°C
Visibility	Forest Green (Neutral)

⚗️ Laboratory Prep

1 Suspend 20.1g in 1L purified water. Mix until dissolved.
2 Heat to boiling to melt the agar.
3 Autoclave at 121°C for 15 minutes.
4 For testing, inoculate two tubes; overlay one with sterile paraffin oil (anaerobic).