Peptone Agar (AS-1318)

Peptone Agar (AS-1318)

$29.00 AUD
Weight
Request a Quote
EMPTY DOM REMOVE PROTECTOR
AuSaMicS Life Science • Basic & Functional Media

Peptone Agar

Simple Solid Medium for Motility, Maintenance & Custom Formulations

Minimal solid medium containing peptone as the principal nitrogen source with no added carbohydrates or indicators. Peptone Agar is widely used for motility testing, maintenance of selected organisms, purity and viability checks, and as a flexible base for customized biochemical or functional media.

AS-1318 🧪 Basic Medium ✓ Customizable Base 🔬 Motility Testing
Use: Motility, maintenance, purity checks Format: Plates, deeps, semi-solid tubes Profile: Low background metabolism

Functional Media Specialist

Simple Composition - Minimal background interference
Motility Ready - Suitable for semi-solid variants
Colony Morphology - Natural pigmentation and growth patterns
Custom Base - Flexible for derived media
Routine Lab Utility - Maintenance and viability checks
Low-Interference Base
Useful when richer media are undesirable

🧪 Complete Formulation & Functional Profile

📋 Standard Composition (per Liter)

Ingredient Concentration Primary Function Contribution
Peptone (tryptic or pancreatic) 10.0 g Nitrogen source Peptides and amino acids for basic growth support
Sodium Chloride 5.0 g Osmotic balance Helps maintain isotonic conditions
Agar 15.0 g (plates) or 3–5 g (semi-solid) Solidifying agent Supports plate culture or motility format
Final pH: 7.0 ± 0.2 at 25°C

📋 Common Variants

Variant Modification Typical Use
Motility Peptone Agar 3–4 g/L agar Motility assessment in semi-solid format
Peptone Agar + TTC 0.02–0.05 g/L TTC Visualization of motile spread as red zones
Salt-Free Peptone Agar NaCl omitted Halosensitive or salt-intolerant strains

⚗️ Functional Design

Minimal Background Activity
No added sugars or indicators to complicate interpretation
Motility-Compatible Format
Semi-solid versions allow migration and spread assessment
Flexible Base Medium
Useful starting point for indole-nitrate, phenylalanine, and related media
Why Peptone Agar? Ideal where reduced nutrient complexity, natural colony appearance, motility testing, or custom formulation flexibility are more important than rapid growth on rich media.

Complete Basic & Functional Media Portfolio

Medium Type Nutrient Level Best Application Motility Suitability Key Advantage
Peptone Agar Simple functional medium Low to moderate Motility, maintenance, custom media base Excellent in semi-solid form Minimal interference and high flexibility
Nutrient Agar General-purpose agar Moderate Routine cultivation Limited Broader everyday recovery
Tryptic Soy Agar Rich general medium High Clinical and routine microbiology Poor for classic motility format Enhanced growth of broader flora
Motility Test Media Semi-solid functional media Low to moderate Dedicated motility testing Excellent Optimized for motility interpretation
Derived Biochemical Media Functional derivative media Variable Biochemical testing Application-specific Built on simple peptone base logic

Strategic Medium Selection for Low-Complexity Culture Work

✅ Peptone Agar Advantages

  • ✓ Simple Composition: Minimal background reactions
  • ✓ Natural Colony Morphology: Good visibility of pigmentation and form
  • ✓ Motility Ready: Semi-solid format supports migration studies
  • ✓ Useful for Maintenance: Suitable for some organisms inhibited by richer media
  • ✓ Customizable: Strong foundation for derivative media

⚠️ Method Considerations

  • ▲ Limited Nutrients: Some fastidious organisms may still need richer media
  • ▲ No Differential Indicators: Interpretation depends on morphology or added supplements
  • ▲ Variant-Dependent Performance: Agar concentration must match the intended use
  • ▲ Not Broadly Enriched: Routine recovery may be lower than with nutrient-rich media

🎯 Best-Fit Applications

  • ◆ Motility Testing: Semi-solid peptone formulations with or without TTC
  • ◆ Culture Maintenance: Pseudomonas, Alcaligenes, Acinetobacter, and related organisms
  • ◆ Purity Checks: Low-interference colony observation
  • ◆ Custom Media Development: Base for specialized biochemical formulations

📊 Application-Specific Performance

Application Peptone Agar Alternative Medium Key Factor
Motility testing Excellent in semi-solid form Dedicated motility media Agar concentration and visualization aid
Natural colony morphology Excellent Nutrient Agar Lower background nutrient complexity
Maintenance of selected proteolytic organisms Very Good Tryptic Soy Agar Reduced richness may be beneficial
Custom biochemical base Excellent Other basal media Simple composition allows easy modification
Fastidious organism recovery Moderate Enriched agars Some strains may still need more nutrients

🔬 Quality Control & Performance Guidance

Typical Organisms:
Pseudomonas, Alcaligenes, Acinetobacter
and other less demanding organisms
Incubation:
35–37°C typical
25–30°C for psychrophilic applications
Motility Format:
3–5 g/L agar
Optional TTC visualization
Preparation:
Standard plate or semi-solid format
Adapt agar concentration to purpose
Positioning: A practical low-complexity medium for general microbiology, motility testing, culture maintenance, and the preparation of customized derivative media in research and routine laboratories.

📋 Technical Specifications

Catalogue Number AS-1309
Medium Type Simple solid basal medium
Standard Agar Content 15.0 g/L
Motility Variant Agar 3–5 g/L
Final pH 7.0 ± 0.2 (at 25°C)
Nutrient Profile Peptone-based, no added carbohydrates or indicators
TTC Variant 0.02–0.05 g/L optional
Sterilization 121°C, 15 minutes
Prepared Plate/Tube Storage 2–8°C, sealed
Recommended Use Period Within 2 months

🧫 Primary Applications

  • ✓ Motility testing in semi-solid format
  • ✓ Maintenance of selected cultures inhibited by richer media
  • ✓ Viability and purity checks
  • ✓ Basal medium for derivative formulations such as indole-nitrate and phenylalanine media

⚗️ Preparation Protocol

  1. 1. Dissolve 10 g peptone, 5 g sodium chloride, and 15 g agar in 1 L purified water.
  2. 2. Adjust pH if necessary.
  3. 3. Autoclave at 121°C for 15 minutes.
  4. 4. Pour plates or dispense into tubes as required.
  5. 5. For motility use, reduce agar concentration to semi-solid levels before sterilization.

📦 Storage & Use

Prepared media: Store plates or tubes at 2–8°C, sealed, and use within 2 months.

Incubation guidance: 35–37°C for many routine organisms, or 25–30°C for psychrophilic applications.

Observation period: Typically 18–48 hours depending on organism and format.

Notice: For general microbiology, motility testing, and culture maintenance only.

AuSaMicS Life Science: Professional microbiology media for general microbiology, functional testing, quality control, research, and customized laboratory workflows. Product information is provided for laboratory and technical evaluation purposes only.

★★★★★
★★★★★
★★★★★
★★★★★

CUSTOMERS ALSO BOUGHT