Skip to product information
Request a Quote
Peptone Saline Solution | Diluent for Microbial Testing – Ausamics
$35.00 AUD
AuSaMicS Life Science • Sample Preparation & Dilution Media
Peptone Saline Solution
Universal Diluent for Food & Environmental Microbiology
Official universal diluent for microbiological examination of foods, feeds, and environmental samples where maximum recovery of stressed or injured microorganisms is required. Peptone Saline Solution provides isotonic protection with light nutritional support, making it ideal for primary dilution, sample homogenisation, and resuscitation prior to plating or enrichment.
AS-1322 🧪 Universal Diluent ✓ ISO Workflow Ready 🔬 Cell Recovery Support
Use: Primary dilution and sample resuscitation Format: Bottles, tubes, rinse and dilution packs Profile: Isotonic and non-interfering
Diluent & Recovery Specialist
Isotonic Protection - 0.85% NaCl supports cell integrity
Gentle Recovery Support - 0.1% peptone helps stressed cells
Broad Compatibility - Suitable before plating or enrichment
Modern Standard Choice - Preferred in current food methods
Routine Laboratory Utility - Food, feed, and environmental workflows
Recovery-Focused Universal Diluent
Designed for injured and stressed microbial cells
🧪 Complete Formulation & Functional Profile
📋 ISO-Type Composition (per Liter)
Final pH: 7.0 ± 0.2 at 25°C
⚗️ Functional Design
Isotonic Stability
0.85% saline protects cells during dilution and handling
0.85% saline protects cells during dilution and handling
Gentle Recovery Support
Low peptone level assists injured organisms without overgrowth
Low peptone level assists injured organisms without overgrowth
Workflow Compatibility
Does not interfere with subsequent enrichment or selective plating
Does not interfere with subsequent enrichment or selective plating
Why Peptone Saline Solution? Ideal where stressed cells must be protected during initial sample dilution, homogenisation, or transfer before quantitative or qualitative microbiological analysis.
Complete Sample Preparation & Diluent Portfolio
Strategic Selection for Sample Dilution & Cell Recovery
✅ Peptone Saline Solution Advantages
- ✓ Universal Use: Suitable for foods, feeds, and environmental samples
- ✓ Excellent Recovery: Supports stressed and injured microorganisms
- ✓ Modern Standard Preference: Favoured over plain saline and many older diluents
- ✓ Broad Compatibility: Does not compromise downstream selective media
- ✓ Routine QC Utility: Useful for swabs, rinses, sponges, and homogenised samples
⚠️ Method Considerations
- ▲ Not an Enrichment Medium: Intended for dilution and recovery, not selective growth
- ▲ Sterility Monitoring Needed: Long storage requires routine sterility checks
- ▲ No Buffering System: Not intended for strong acid or alkali neutralisation
- ▲ Application Must Match Standard: Some methods still specify alternative diluents or pre-enrichment media
🎯 Best-Fit Applications
- ◆ Primary 1:10 Food Dilution: Enumeration and enrichment preparation
- ◆ Environmental Sampling: Swabs, sponges, and carcass rinses
- ◆ Stressed Cell Recovery: Heat-, freeze-, acid-, or sanitizer-injured cells
- ◆ Routine Food QC: Standardized sample preparation workflows
📊 Typical Recovery Utility
🔬 Quality Control & Performance Guidance
Typical Use:
Primary 1:10 dilution
Sample homogenisation and recovery
Primary 1:10 dilution
Sample homogenisation and recovery
Standard Volumes:
9 mL and 90 mL common
225 mL and 450 mL for ISO sample sizes
9 mL and 90 mL common
225 mL and 450 mL for ISO sample sizes
Recovery Guidance:
20–25°C pre-warmed use
30–60 min resuscitation where required
20–25°C pre-warmed use
30–60 min resuscitation where required
Storage:
Room temperature or 2–8°C
Check sterility monthly during extended storage
Room temperature or 2–8°C
Check sterility monthly during extended storage
Positioning: A modern standard universal diluent for food, feed, and environmental microbiology laboratories seeking reliable recovery of stressed microorganisms without compromising downstream analysis.
📋 Technical Specifications
| Catalogue Number | AS-1322 |
| Product Type | Universal isotonic diluent |
| Peptone Content | 1.0 g/L |
| NaCl Content | 8.5 g/L |
| Final pH | 7.0 ± 0.2 (at 25°C) |
| Typical Pack Formats | 9 mL, 90 mL, 225 mL, 450 mL preparations |
| Sterilization | 121°C for 15 minutes |
| Alternative Preparation | May be membrane-filtered for heat-sensitive uses |
| Storage | Room temperature or 2–8°C |
| Recommended Use Period | Up to 6 months with sterility monitoring |
🧫 Primary Applications
- ✓ Primary dilution of food samples before enumeration or enrichment
- ✓ Environmental swab, sponge, and carcass rinse dilution
- ✓ Recovery of freeze-, heat-, acid-, or sanitizer-injured cells
- ✓ General sample preparation in modern food microbiology methods
⚗️ Preparation & ISO Use Guidance
- 1. Dissolve 1.0 g peptone and 8.5 g NaCl in 1 L purified water.
- 2. Dispense into bottles or tubes as required, commonly 90 mL or 9 mL.
- 3. Autoclave at 121°C for 15 minutes.
- 4. Add sample to pre-warmed diluent according to the required ratio, such as 25 g to 225 mL.
- 5. Homogenise and allow short room-temperature resuscitation before further dilution or plating where required.
📦 Storage & Intended Use
Prepared solution: Store at room temperature or 2–8°C.
Stability: Use within 6 months, with monthly sterility checks during extended storage.
Workflow note: Designed to avoid interference with downstream selective and enumeration media.
Notice: For food, feed, and environmental microbiology only.
AuSaMicS Life Science: Professional microbiology media and diluents for food safety, environmental monitoring, quality control, research, and standardized sample preparation workflows. Product information is provided for laboratory and technical evaluation purposes only.