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PPLO Agar (Mycoplasma Agar Base) | AS-1332
$45.00 AUD
Microbiological Culture Media · Mycoplasmology · Reference Grade
PPLO Agar (Mycoplasma Agar Base)
Compliance: USP <63> · EP 2.6.7 · FDA-BAM Ch. 23
Pharmacopoeia Grade
Reference Solid Medium
Cell Culture Tested
Batch Traceable
AuSaMicS
Laboratory Division
Cat No.
AS-1332
Base pH
7.8 ± 0.2
Agar Content
0.8–1.2%
Storage
2–25°C (Dehydrated)
Reference
Morton et al.
Description
PPLO Agar is the international reference solid medium for the primary isolation, cultivation, and enumeration of Mycoplasma species (including M. pneumoniae, M. hominis, M. genitalium), Ureaplasma spp., and Acholeplasma laidlawii. It is designed to support the growth of fastidious mycoplasmas from clinical specimens, cell cultures, and pharmaceutical biologics.
Applications
Pharmaceutical Compliance
Official medium for USP <63> and EP 2.6.7 (28-day culture method) mycoplasma testing in biologics and vaccines.
Cell Culture Quality
Standardized detection of mycoplasma contamination in cell lines and viral harvests per FDA-BAM guidelines.
Clinical Isolation
Primary isolation of pathogenic mycoplasmas from respiratory, urogenital, and other clinical specimens.
Key Characteristics
The medium features an enriched beef heart infusion and proteose peptone base. The very low agar concentration (0.8–1.2%) is critical, as it facilitates the penetration of colonies into the medium, resulting in the characteristic "fried-egg" morphology identifiable under low-power microscopy.
Preparation Method
| Base Suspension | Suspend 21–25 g in 800 mL purified water. Boil to dissolve. |
| Sterilization | Autoclave at 121°C for 15 minutes. |
| Enrichment | Cool to 50°C. Aseptically add 200 mL sterile serum + 10–20 mL yeast extract. |
| Additives | Glucose/Phenol Red for M. pneumoniae; Penicillin/Ampicillin for bacterial inhibition. |
Incubation Parameters
Incubate at 36 ± 1°C in a high-humidity environment for 7–28 days. For M. pneumoniae, an atmosphere of 5–10% CO₂ is essential. Examine plates under a stereo microscope (10–40x) beginning on day 3.
Morphology Chart
| Organism | Appearance | Size |
|---|---|---|
| Mycoplasma spp. | Typical "fried-egg" (embedded center) | 50–300 µm |
| Acholeplasma | Large colonies, lacking peripheral zone | Varies |
| Ureaplasma | Tiny "sea-urchin" or brown-black (if urea added) | Very small |
Base Composition (per Liter)
| Beef Heart Infusion Solids | 6.0 g |
| Proteose Peptone | 10.0 g |
| Sodium Chloride | 5.0 g |
| Agar | 8.0 – 12.0 g |
| Final pH (Base) | 7.8 ± 0.2 at 25°C |
Essential Supplements (Post-Autoclave)
● Sterile Horse or Foetal Bovine Serum (10–20%)
● Fresh Yeast Extract (25% solution, 1–2%)
Technical transparency is central to the Ausamics quality standard. Documentation for AS-1332 is available for laboratory review.
TDS
Technical Data Sheet containing full specifications.
SDS
Safety Data Sheet for dehydrated base.
COA
Batch-specific Certificate of Analysis.
AuSaMicS Laboratory Division provides professional-grade culture media for clinical, pharmaceutical, and veterinary mycoplasmology. Prepared plates contain perishable serum and should be used within 7 days of assembly.
Ausamics · Reference Microbiology Media · Cat No: AS-1332