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PPLO Broth | Mycoplasma Broth Base
$45.00 AUD
PPLO Broth; Mycoplasma Broth Base - AS-1333
🔬 Specialized Mycoplasma Culture Medium
Professional-grade liquid culture medium specifically formulated for the cultivation and isolation of Mycoplasma species and other pleuropneumonia-like organisms (PPLO) requiring specialized nutritional conditions.
🔬 Technical Overview & Biochemistry
PPLO Broth represents a sophisticated nutritional platform designed for the unique metabolic requirements of Mycoplasma species. These wall-less bacteria require enriched media with specific lipids, sterols, and growth factors that cannot be synthesized by the organisms themselves due to their minimal genome size and metabolic capabilities.
Metabolic Support: The formulation provides essential nutrients including complex peptones, serum proteins, and lipid supplements necessary for mycoplasma cell membrane synthesis and maintenance. The medium's balanced osmolarity and pH buffering system create optimal conditions for these osmotically sensitive organisms.
Key Features & Applications
🧬 Mycoplasma Cultivation
Specialized formulation supports growth of fastidious Mycoplasma species with essential lipids and sterols for membrane synthesis.
🔍 Contamination Detection
Critical for cell culture quality control and mycoplasma contamination screening in biopharmaceutical and research applications.
🩺 Clinical Diagnostics
Essential for isolation and identification of Mycoplasma pneumoniae and other clinically relevant mycoplasma species.
🐄 Veterinary Applications
Supports diagnosis of mycoplasma infections in livestock and companion animals, including respiratory and urogenital diseases.
Additional Details
TDS
SDS
COA
Cross-Reference / Equivalent Products
| Manufacturer | Product Name | Catalogue Number | Notes |
|---|---|---|---|
| Oxoid | PPLO Broth Base | CM0403 | Standard formulation |
| BD Difco | PPLO Broth wo CV | 255420 | Without crystal violet |
| Merck | PPLO Broth | 110346 | Equivalent formulation |
| Thermo Fisher | Mycoplasma Broth Base | R453802 | Alternative formulation |
⚠️ Important Supplementation Required
This is a BASE medium that requires supplementation for optimal mycoplasma growth:
- Horse Serum: Add 15-20% sterile horse serum
- Fresh Yeast Extract: Add 10% fresh yeast extract solution (25% w/v)
- Selective Agents (optional): Penicillin G, thallium acetate, or methylene blue
🦠 Target Organisms & Applications
- Mycoplasma pneumoniae - Atypical pneumonia diagnosis
- Mycoplasma hominis - Urogenital tract infections
- Ureaplasma urealyticum - Urogenital and neonatal infections
- Mycoplasma bovis - Bovine respiratory disease complex
- Mycoplasma hyopneumoniae - Swine enzootic pneumonia
- Cell Culture Contamination: Detection in mammalian cell lines
- L-forms: Cell wall-deficient bacterial variants
🔍 Related Search Terms
Common searches: mycoplasma culture medium, PPLO broth base, mycoplasma pneumoniae isolation, cell culture contamination testing, mycoplasma detection medium, pleuropneumonia organisms culture, veterinary mycoplasma diagnostics, mycoplasma broth supplementation, wall-less bacteria culture, L-form cultivation medium
Technical Specifications
| Parameter | Specification | Method |
|---|---|---|
| pH (25°C) | 7.8 ± 0.2 | Potentiometric |
| Appearance | Pink to red, clear solution | Visual inspection |
| Osmolarity | 290 ± 20 mOsm/kg | Freezing point depression |
| Endotoxin Level | <0.1 EU/mL | LAL assay |
| Preparation | 25.1g per 1000mL | Standard protocol |
| Shelf Life | 36 months | Stability testing |
Preparation Instructions
- Base Preparation: Suspend 25.1g in 1000mL distilled water
- Dissolution: Heat gently with stirring until completely dissolved
- pH Adjustment: Adjust pH to 7.8 ± 0.1 if necessary
- Sterilization: Autoclave at 121°C for 15 minutes
- Cooling: Cool to room temperature aseptically
- Supplementation: Add sterile supplements (serum, yeast extract) as required
- Final pH Check: Verify pH 7.8 ± 0.2 after supplementation
Technical Data Sheet - PPLO Broth Base
📄 TDS Download Available
Complete technical specifications, supplementation protocols, and validation data
Complete technical specifications, supplementation protocols, and validation data
The Technical Data Sheet provides comprehensive information including:
- Detailed composition and nutritional requirements
- Supplementation protocols for different mycoplasma species
- Performance validation with reference strains and clinical isolates
- Quality control procedures and acceptance criteria
- Troubleshooting guide for mycoplasma cultivation
- Literature references and application protocols
Safety Data Sheet - PPLO Broth Base
⚠️ SDS Download Available
Complete safety information and handling procedures (16-section GHS format)
Complete safety information and handling procedures (16-section GHS format)
The Safety Data Sheet includes:
- Hazard identification and GHS classification
- Safe handling procedures for laboratory use
- Storage requirements and incompatible materials
- Emergency response and first aid measures
- Personal protective equipment recommendations
- Disposal considerations and regulatory compliance
Classification: Not classified as hazardous according to Australian WHS regulations. Contains phenol red - avoid skin/eye contact.
Certificate of Analysis - PPLO Broth Base
✅ COA Download Available
Batch-specific quality control results and mycoplasma performance validation
Batch-specific quality control results and mycoplasma performance validation
Each Certificate of Analysis provides:
- Batch-specific test results for all physical and chemical parameters
- Mycoplasma growth performance with reference strains
- pH measurement and osmolarity verification
- Endotoxin level confirmation for cell culture applications
- Sterility testing results and contamination screening
- Quality assurance certification and batch release authorization
QC Testing: Every batch tested with standardized mycoplasma reference strains and supplementation protocols validated.