PSB Broth Base (Peptone Sorbitol Bile) | AS-1336 | Yersinia Enrichment

$95.00 AUD

AuSaMicS Life Science • Pathogen Enrichment

PSB Broth Base

Peptone Sorbitol Bile Broth for Yersinia

A highly effective enrichment medium designed for the selective recovery of Yersinia enterocolitica from food, water, and clinical specimens, compliant with ISO and FDA-BAM standards.

AS-1336 🧪 Selective Enrichment ✓ ISO 10273 🔬 Food Safety Standard

Target: Yersinia enterocolitica Indicator: Sorbitol Fermentation Base: Bile Salt Selective

Detection Specialist

High Recovery - Optimized for stressed cells

Dual Action - Enrichment & Selectivity

Buffer Stability - Maintains pH for growth

Versatile - Cold & Room temp protocols

Inhibitory Efficiency

Bile Salts No. 3 Included

🧪 Complete Formulation Profile

📋 Ingredient Composition (per Liter)

Ingredient	Concentration	Primary Function
Peptone (Enzymatic)	5.0 g	Nitrogen & Growth Nutrients
Sorbitol	10.0 g	Fermentable Carbohydrate
Bile Salts No. 3	1.5 g	Inhibitor of Gram-positive flora
Sodium Chloride	5.0 g	Osmotic Balance
Phosphate Buffer System	7.0 g	pH Maintenance

🔬 Regulatory Compliance & Application

PSB Broth Base is essential for the detection of Yersinia enterocolitica, which is often present in low numbers alongside heavy competing microflora in food products (particularly pork).

Cold Enrichment
Supports the ability of Yersinia to grow at 4°C, providing a competitive advantage over mesophiles.

Selective Suppression
Bile salts effectively suppress the growth of non-target Gram-positive organisms.

📋 Technical Specifications

Cat Number	AS-1336
Final pH	7.6 ± 0.2
Appearance	Beige, free-flowing
Storage	2-30°C

⚗️ Preparation Guide

1 Suspend 28.5g in 1L distilled water.
2 Mix well and dissolve by heating if necessary.
3 Autoclave at 121°C for 15 minutes.
4 Cool to room temperature before inoculation.