Rogosa Agar

Overview

Rogosa Agar is a highly selective medium engineered for the isolation and enumeration of Lactobacillus species — especially in dairy products, fermented foods, and probiotic formulations.

Its formulation combines low pH and selective agents (sodium acetate + organic acids) to aggressively suppress competing bacteria, while still supporting the growth of fastidious lactobacilli.

The result: clean plates, minimal background growth, and reliable Lactobacillus recovery — even in complex samples.

Why Labs Choose Rogosa Agar

✔ Strong selectivity — suppresses unwanted flora
✔ Optimised for Lactobacillus recovery
✔ Clean, low-background plates
✔ Ideal for probiotic and fermentation work
✔ Consistent, reproducible results

Where It’s Used

Dairy & Food Microbiology
• Enumeration of Lactobacillus in yoghurt, cheese, and fermented foods
• Microbial quality control in food production

Probiotic Industry
• Viability testing of probiotic strains
• Strain isolation and validation

Environmental Monitoring
• Detection of lactic acid bacteria in processing environments

Research & Fermentation Studies
• Growth and characterisation of Lactobacillus species
• Fermentation optimisation studies

Why This Medium Matters

In probiotic and food testing, contamination is the biggest problem.

Rogosa Agar solves this by:
→ Suppressing competing microbes at low pH
→ Favouring Lactobacillus growth
→ Reducing false positives and overgrowth

That means more accurate counts, cleaner isolation, and faster workflows.

Composition (per litre)

Peptone / Proteose Peptone – 10.0 g
Yeast Extract – 5.0 g
Glucose – 20.0 g
Sodium Acetate – 20.0 g
Agar – 15.0 g

Final pH: 5.4 ± 0.2 at 25 °C

Preparation

Dissolve 70 g in 1 L purified water
Heat with agitation until fully dissolved
Autoclave at 121 °C for 15 minutes
Cool to 45–50 °C before pouring

Incubation & Interpretation

Temperature: 30–37 °C
Time: 48–72 hours (up to 5 days for slow strains)

Results:
• Growth indicates presence of Lactobacillus spp.
• Colony morphology supports species differentiation

Storage

Powder: 15–30 °C, dry conditions
Prepared plates: 2–8 °C
Protect from light and moisture

Compliance

✔ COA available
✔ SDS available
✔ Suitable for food microbiology and research labs

Intended Use

For laboratory use only.
Not for human, animal, or food consumption.

Why Buy from Ausamics

✔ Local Australian stock — no import delays
✔ Designed for food & probiotic labs
✔ Competitive pricing vs global brands
✔ Fast dispatch from Melbourne