Safranin O Powder (CAS 477-73-6) | Gram Stain Counterstain | Lab-Grade Biological Dye | Ausamics
Safranin O Powder
Premium Biological Stain for Gram Staining, Histology & Microscopy
Catalog Number: ASD-623
CAS Number: 477-73-6
HS Code: 3204.13.00
Product Overview
Safranin O Powder is a high-purity cationic biological dye widely used in microbiology, histology, and cytology laboratories. The dye exhibits strong affinity for acidic cellular components, enabling clear visual contrast in microscopic staining protocols.
It is most commonly used as the counterstain in Gram staining, allowing differentiation of Gram-negative bacteria.
Manufactured under controlled conditions, Ausamics Safranin O ensures consistent staining performance and batch-to-batch reproducibility.
Key Applications
Gram Staining (Microbiology)
Safranin O functions as the counterstain in Gram staining protocols.
• Gram-positive bacteria appear purple
• Gram-negative bacteria appear red/pink
This staining contrast allows rapid bacterial classification in microbiology labs.
Histology
Safranin O is commonly used to stain:
• Cartilage matrix
• Proteoglycans
• Chondrocytes
• Tissue sections
It is widely used in orthopedic research and pathology studies.
Cytology & Microscopy
Safranin O improves visualization in:
• Cytological smears
• Fixed cell preparations
• Tissue imaging under brightfield microscopy
Industrial Applications
Safranin O is also used in:
• Textile dyeing research
• Leather colouring
• Material science studies
Product Features
✔ High-purity laboratory dye
✔ Excellent Gram stain counterstain
✔ Consistent staining intensity
✔ Fine powder for rapid dissolution
✔ Reliable batch-to-batch performance
Specifications
| Property | Value |
|---|---|
| Product Name | Safranin O Powder |
| Catalog Number | ASC-1011 |
| CAS Number | 477-73-6 |
| HS Code | 3204.13.00 |
| Molecular Formula | C20H19ClN4 |
| Molecular Weight | 350.85 g/mol |
| Appearance | Red crystalline powder |
| Solubility | Soluble in water and ethanol |
| Grade | Laboratory / Research Grade |
Storage
Store tightly sealed in a dry, cool environment (15-25°C).
Protect from light and moisture.
Safety
Use standard laboratory protective equipment:
• Lab coat
• Gloves
• Safety goggles
For laboratory use only.