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Schaedler Broth Base | Schaedler Anaerobe Broth | AS‑1344
$45.00 AUD
AuSaMicS Life Science • Anaerobic & Fastidious Organism Media
Schaedler Broth Base
Schaedler Anaerobe Broth
The gold-standard non-selective enrichment broth for cultivation of obligate anaerobes, microaerophiles, and nutritionally demanding fastidious organisms. Schaedler's uniquely balanced formulation — enriched with glucose, haemin, and vitamin K₁ — delivers outstanding recovery of the full spectrum of clinically and research-relevant anaerobic flora.
Cat #: AS-1344
CLSI M11 / USP <71>
✓ Australian Stock
🫧 Anaerobic Cultivation
🚀 Same-Day Dispatch: Australian Stock
🔬 Non-selective: Full-spectrum anaerobe recovery
🌡️ 35–37°C Anaerobic: 24–72 h incubation
🏆 Anaerobe Cultivation Specialist
🫧 Non-selective Base — Full-spectrum anaerobe recovery
💉 Haemin + Vit K₁ — Essential anaerobe growth factors
📋 Complete Package — COA + SDS included
⚡ Fast Supply — Ships within 24h
📞 Expert Support — Protocol optimization
Blood Supplementable
Add 5–10% defibrinated blood for enhanced recovery of fastidious anaerobes
🔬 Technical Overview & Biochemistry
Schaedler Broth Base is a non-selective, highly enriched anaerobic culture medium originally developed by Schaedler, Dubos, and Costello (1965) for the cultivation of the intestinal microflora in germ-free animal models. Its exceptional nutritive richness and carefully balanced growth factor profile have since established it as one of the most widely used media for the recovery and cultivation of obligate anaerobes and nutritionally demanding microaerophiles across clinical, pharmaceutical, and research applications.
Key Nutritive & Growth Factor Components:
Tryptic Digest of Casein — Provides essential amino acids, peptides, and nitrogen sources required by fastidious anaerobes with complex nutritional requirements
Yeast Extract — Supplies B-vitamins, nucleotide precursors, minerals, and soluble growth factors critical for anaerobe biosynthesis
Glucose — Primary fermentable carbohydrate energy source; supports rapid growth and reduces the medium's oxidation-reduction potential (Eh), creating a more reduced microenvironment
Haemin (Haematin) — Essential porphyrin growth factor required by many obligate anaerobes including Bacteroides fragilis group, Prevotella, and Porphyromonas spp. that cannot synthesise haem de novo
Vitamin K₁ (Phylloquinone) — Key menaquinone precursor required as an essential electron carrier by black-pigmented anaerobes and other obligate anaerobes
Cysteine Hydrochloride — Reducing agent; lowers the redox potential (Eh) of the medium to values compatible with obligate anaerobe growth (Eh ≤ −150 mV)
Tryptic Digest of Casein — Provides essential amino acids, peptides, and nitrogen sources required by fastidious anaerobes with complex nutritional requirements
Yeast Extract — Supplies B-vitamins, nucleotide precursors, minerals, and soluble growth factors critical for anaerobe biosynthesis
Glucose — Primary fermentable carbohydrate energy source; supports rapid growth and reduces the medium's oxidation-reduction potential (Eh), creating a more reduced microenvironment
Haemin (Haematin) — Essential porphyrin growth factor required by many obligate anaerobes including Bacteroides fragilis group, Prevotella, and Porphyromonas spp. that cannot synthesise haem de novo
Vitamin K₁ (Phylloquinone) — Key menaquinone precursor required as an essential electron carrier by black-pigmented anaerobes and other obligate anaerobes
Cysteine Hydrochloride — Reducing agent; lowers the redox potential (Eh) of the medium to values compatible with obligate anaerobe growth (Eh ≤ −150 mV)
Key Advantage: The combination of haemin, vitamin K₁, cysteine, and glucose makes Schaedler Broth one of the most supportive non-selective anaerobe media available — capable of recovering organisms that fail to grow in less enriched broths such as Thioglycollate or Cooked Meat Medium.
⚙️ Mode of Action
Schaedler Broth creates a highly reduced chemical environment through the combined action of glucose fermentation (which depletes dissolved oxygen and lowers pH slightly), cysteine (a thiol-based chemical reductant that scavenges residual oxygen and maintains low Eh), and the rich peptide matrix that buffers against toxic metabolite accumulation. Haemin supplies the porphyrin ring structure required for cytochrome and catalase biosynthesis by organisms that cannot synthesise it independently, while vitamin K₁ supports the menaquinone-dependent electron transport systems of fastidious anaerobes. Together these factors enable recovery of the most nutritionally demanding obligate anaerobes — organisms that are routinely missed by less enriched media — making Schaedler Broth the preferred non-selective enrichment base for clinical anaerobe culture, pharmaceutical sterility testing, and gut microbiome research applications.
📊 Anaerobic Enrichment Media Comparison
🦠 Target Organisms — Supported Growth
🔬 Obligate Anaerobes — Gram-negative
- Bacteroides fragilis group
- Prevotella spp. (including pigmented)
- Porphyromonas spp.
- Fusobacterium nucleatum
- Veillonella spp.
🔬 Obligate Anaerobes — Gram-positive
- Clostridium spp. (non-SRC)
- Peptostreptococcus / Peptococcus spp.
- Eggerthella lenta
- Propionibacterium spp.
- Actinomyces spp.
🔬 Microaerophiles & Capnophiles
- Campylobacter spp.
- Helicobacter pylori
- Streptococcus (microaerophilic)
- Haemophilus spp.
- Neisseria spp.
🔬 Research & Gut Microbiome Flora
- Bifidobacterium spp.
- Lactobacillus spp. (obligate anaerobes)
- Ruminococcus spp.
- Faecalibacterium prausnitzii
- Akkermansia muciniphila
🧬 Applications
🏥 Clinical Microbiology
Primary enrichment broth for anaerobic cultures from wound specimens, abscesses, blood, body fluids, and tissue biopsies. Supports recovery of clinically significant anaerobes that may be present in low numbers or physiologically stressed.
💊 Pharmaceutical Sterility Testing
Recommended as a supplementary or alternative anaerobe medium in sterility testing workflows per USP <71> and BP Appendix XVI — particularly for products where thioglycollate medium may be inhibitory or insufficiently enriched.
🧫 Gut Microbiome Research
Standard liquid medium for cultivation of intestinal anaerobes in microbiome studies — supports growth of the full spectrum of dominant gut flora including Bacteroides, Bifidobacterium, Faecalibacterium, and butyrate producers.
🩺 Antimicrobial Susceptibility Testing
Base broth for anaerobe MIC determination and susceptibility testing per CLSI M11 guidelines — supplemented with haemin and vitamin K₁ as required by the standard for reliable MIC endpoint reading.
Additional Laboratory Applications:
- Blood Culture Enrichment: Anaerobic blood culture bottle base with 5–10% blood
- Veterinary Diagnostics: Anaerobe culture from animal tissue and abscess specimens
- Probiotic Development: Cultivation of probiotic anaerobic strains for QC and R&D
- Anaerobe Preservation: Short to medium-term maintenance of obligate anaerobe cultures
- NATA Accredited Labs: Clinical microbiology anaerobe culture panels
- Animal Models: Germ-free and gnotobiotic animal intestinal microflora studies
📋 Recommended Procedure
1️⃣
Prepare Medium
Dissolve 28.0 g/L in purified water. Mix well. Dispense into tubes or bottles, filling to ≥2/3 capacity to minimise headspace.
2️⃣
Sterilise
Autoclave 121°C / 15 min. Cool to room temperature before use. Freshly prepared medium should be used within 4 h or pre-reduced in anaerobic cabinet overnight.
3️⃣
Optional: Add Blood
For enhanced recovery of fastidious anaerobes: aseptically add 5–10% sterile defibrinated blood to cooled medium (≤50°C).
4️⃣
Inoculate
Inoculate with sample (clinical specimen, food homogenate, or culture). Minimise air exposure during inoculation.
5️⃣
Incubate Anaerobically
35–37°C for 24–72 h under strict anaerobic conditions. Examine at 24 h intervals for turbidity.
6️⃣
Subculture & ID
From turbid broth, subculture to appropriate selective or non-selective anaerobe agar (e.g., Schaedler Agar, KVBA, Neomycin Blood Agar) for isolation and identification.
🫙 Anaerobic Incubation Options
Anaerobic Chamber / Glove Box: Gold standard — maintains <1 ppm O₂; all inoculation performed inside chamber for maximum recovery
Anaerobic Jars + Gas Packs: Anaerogen/AnaeroGen sachets; suitable for routine clinical and food labs
Sealed Anaerobic Pouches: Convenient for small sample numbers; single-use sealed pouches with catalyst
Pre-reduced Medium: Place prepared tubes in anaerobic atmosphere overnight before inoculation — improves recovery of extremely oxygen-sensitive organisms
⚠️ Important: Minimise oxygen exposure at all stages — inoculate quickly, cap tubes immediately, and establish anaerobic conditions within 15 minutes of inoculation. For extremely oxygen-sensitive organisms (e.g., Faecalibacterium prausnitzii), pre-reduce the medium for at least 24 h in an anaerobic atmosphere before inoculation.
💡 Protocol Optimization Guidelines
Preparation:
Dissolve 28.0 g/L in purified water. Autoclave 121°C / 15 min. Fill containers to ≥2/3 capacity — minimise headspace to reduce O₂ exposure.
Dissolve 28.0 g/L in purified water. Autoclave 121°C / 15 min. Fill containers to ≥2/3 capacity — minimise headspace to reduce O₂ exposure.
pH (25°C):
7.6 ± 0.2 after sterilisation. A slightly alkaline pH favours most clinically relevant anaerobes.
7.6 ± 0.2 after sterilisation. A slightly alkaline pH favours most clinically relevant anaerobes.
Blood Supplement:
Add 5–10% defibrinated horse or sheep blood aseptically to cooled medium (≤50°C) for enhanced recovery of Bacteroides, pigmented anaerobes, and other fastidious species.
Add 5–10% defibrinated horse or sheep blood aseptically to cooled medium (≤50°C) for enhanced recovery of Bacteroides, pigmented anaerobes, and other fastidious species.
Storage:
Prepared broth: 2–8°C, use within 2 weeks in sealed containers. Dehydrated powder: below 25°C, dry and dark. Discard if medium turns pink (oxidation indicator).
Prepared broth: 2–8°C, use within 2 weeks in sealed containers. Dehydrated powder: below 25°C, dry and dark. Discard if medium turns pink (oxidation indicator).
📋 Technical Specifications
| Catalogue Number | AS-1344 |
| Common Name | Schaedler Anaerobe Broth / SAB |
| Format | Dehydrated powder |
| Reconstitution | 28.0 g/L in purified water |
| pH (25°C) | 7.6 ± 0.2 |
| Incubation Temp | 35–37°C |
| Incubation Time | 24–72 hours (examine at 24 h intervals) |
| Atmosphere | Strictly anaerobic |
| Positive Result | Turbid growth |
| Key Growth Factors | Haemin, Vitamin K₁, Cysteine, Glucose |
| Blood Supplementable | Yes — 5–10% defibrinated blood |
| Sterilisation | Autoclave 121°C / 15 min |
| HS Code | 3821.00.00 |
✅ Quality Control Performance
| Test Organism | ATCC | Expected Result | CFU Spec |
|---|---|---|---|
| Bacteroides fragilis | 25285 | Good growth, turbid | ≤100 CFU |
| Clostridium perfringens | 13124 | Good growth, turbid | ≤100 CFU |
| Peptostreptococcus anaerobius | 27337 | Good growth, turbid | ≤100 CFU |
| Bifidobacterium longum | 15707 | Good growth, turbid | ≤100 CFU |
QC performed under strict anaerobic conditions. All organisms demonstrate growth as turbidity in liquid medium.
🧪 Typical Formulation (per litre)
| Tryptic Digest of Casein | 17.0 g |
| Soya Peptone | 3.0 g |
| Yeast Extract | 5.0 g |
| Glucose | 5.0 g |
| Sodium Chloride | 1.7 g |
| L-Cysteine Hydrochloride | 0.4 g |
| Haemin (Hemin) | 0.01 g |
| Vitamin K₁ (Phylloquinone) | 0.0005 g |
* Formulation may vary slightly between lot numbers. Refer to the current COA for exact composition.
📜 Standards & Regulatory Compliance
- ✓ CLSI M11 — Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria
- ✓ USP <71> — Sterility Testing of pharmaceutical products (anaerobe medium)
- ✓ BP Appendix XVI — British Pharmacopoeia sterility test media
- ✓ ISO 11133:2014 — Preparation, production, storage, and performance testing of culture media
- ✓ TGA / TGO 77 — Australian pharmaceutical microbiology requirements
- ✓ NATA Accreditation — Suitable for NATA-accredited clinical and pharmaceutical laboratories
📝 Alternative Names
Schaedler Broth Base
Schaedler Anaerobe Broth (SAB)
Schaedler Enrichment Broth
Schaedler Blood Broth Base
Schaedler-Dubos-Costello Broth
Fastidious Anaerobe Broth (FAB) — related
🔄 Cross-Reference / Equivalent Products
Cross-references are provided for convenience. Catalogue numbers are trademarks of their respective owners. AuSaMicS products are manufactured and quality-tested independently.
🧬 Complete Anaerobic Microbiology System
Anaerobe Enrichment & Non-selective Media
Selective & Isolation Agar for Subculture
Need Anaerobic Microbiology Method Support?
Our microbiologists can assist with anaerobe cultivation protocol design, CLSI M11 susceptibility testing setup, USP <71> pharmaceutical sterility testing workflows, NATA compliance documentation, and gut microbiome research media selection.
For laboratory, research, and industrial use only. Not for food, feed, household, cosmetic, therapeutic, or personal use. Strict anaerobic conditions are required for valid results — aerobic incubation will not support obligate anaerobe growth. Results should be interpreted by qualified laboratory personnel in accordance with applicable standard methods.
AuSaMicS Pty Ltd | ABN: 56 676 640 467 | 31 Longview CT, Thomastown VIC 3074, Australia
www.ausamics.com.au | support@ausamics.com.au | +61 412 520 598
Same-day dispatch • Australian stock • Full documentation included