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Tryptic Soy Broth with Lecithin and Polysorbate 80 (MCT Broth- SCDLP80) Broth - AS-1433
$95.00 AUD
Tryptic Soy Broth with Lecithin and Polysorbate 80
Neutralising Liquid Enrichment Medium | MCT Broth | SCDLP80 Broth | Cat. No. AS-1433
Cat. No. AS-1433 pH 7.3 ± 0.2 ~31 g/L Lecithin 0.7 g/L + Tween 80 5.0 g/L ISO 21149 | ISO 18415 | USP LIQUID / BROTH FORM 🇦🇺 Made in Melbourne ⚡ Same-Week Dispatch
📄 Full DocumentationCOA, TDS & SDS every batch
🇦🇺 Australian StockNo import delays
⚡ Same-Week DispatchMelbourne warehouse
🔬 Technical SupportDirect from our team
Overview & Mode of Action
Tryptic Soy Broth with Lecithin and Polysorbate 80 (AS-1433) — also known as MCT Broth (Microbial Content Test Broth) or SCDLP80 Broth — is a non-selective, neutralising liquid enrichment medium for microbiological QC of cosmetics, pharmaceuticals, and personal care products containing disinfectant residues.
The TSB nutritional base (casein + soy peptone) supports recovery of a broad spectrum of bacteria, yeasts, and moulds including stressed and sub-lethally injured cells. Lecithin (0.7 g/L) neutralises QACs and chlorhexidine. Polysorbate 80 (5.0 g/L) neutralises phenolics, hexachlorophene, parabens, and formalin — preventing false-negative results caused by disinfectant carryover.
ⓘ Agar version available: AS-1351 — Tryptic Soy Agar with Lecithin and Polysorbate 80 (same formula, solid form for plating and colony counting)
Applications
🌿
Environmental Monitoring
Cleanroom & surface sampling
🧪
Cosmetics QC
ISO 21149 / ISO 18415
💊
Pharma QC
USP <61> / EP 2.6.12
🏭
Personal Care
Preserved formulations
🧦
Sanitation Validation
Post-disinfection recovery
🔬
Research
Custom neutralisation studies
Formula (Verified — Merck Microbiology Manual)
| Ingredient | g / L | Function | Neutralises / Role |
|---|---|---|---|
| Casein Peptone (Tryptone) | 15.0 | Primary nitrogen source | Amino acids, peptides |
| Soy Peptone | 5.0 | Supplementary nitrogen + vitamins | B-vitamins, growth factors |
| Sodium Chloride (NaCl) | 5.0 | Osmotic balance | Physiological ionic strength |
| Lecithin (Soya) | 0.7 | QAC neutraliser | QACs, benzalkonium chloride, chlorhexidine |
| Polysorbate 80 (Tween® 80) | 5.0 | Phenolic neutraliser | Phenolics, hexachlorophene, parabens, formalin |
| Total | ~31 g/L | pH 7.3 ± 0.2 at 25°C | Autoclave 121°C / 15 min |
Comparison — TSA + Lecithin + Polysorbate 80 vs Similar Media
| Medium | Form | Neutraliser | Primary Use | Standard |
|---|---|---|---|---|
| TSB with Lecithin & Polysorbate 80 (AS-1433) ★ | Liquid (broth) | Lecithin 0.7g + Tween 80 5g | Surface plating, env. monitoring, cosmetics QC | ISO 21149; USP |
| TSA with Lecithin & Polysorbate 80 (AS-1351) | Solid (agar) | Lecithin 0.7g + Tween 80 5g | Surface plating, env. monitoring, colony counting | ISO 21149; USP |
| Letheen Agar (AS-1387) | Solid (agar) | Lecithin 0.7g + Tween 80 5g | Disinfectant efficacy testing, surface recovery | AOAC; FDA BAM |
| Tryptic Soy Agar (AS-1372) | Solid (agar) | None | General cultivation (no neutralisation) | ISO 11133; USP |
| D/E Neutralising Agar | Solid (agar) | Multi-neutraliser system | Broad disinfectant range | AOAC; ASTM |
FAQs
❓ What is the difference between AS-1433 (Broth) and AS-1351 (Agar)?
Both have identical formulations (Casein 15g, Soy 5g, NaCl 5g, Lecithin 0.7g, Polysorbate 80 5g). AS-1433 is the liquid broth used for enrichment, sample dilution, and liquid-phase recovery. AS-1351 contains 15 g/L agar for plating and colony counting. Use both together: enrich in AS-1433, then plate on AS-1351.
❓ Is AS-1433 equivalent to Merck 1.10692 or Oxoid CM0693?
Yes — AS-1433 is formulated to the same reference standard (Casein 15g, Soy 5g, NaCl 5g, Lecithin 0.7g, Polysorbate 80 5g, pH 7.3). Full COA included with every batch.
❓ What disinfectants does this medium neutralise?
Lecithin (0.7 g/L) neutralises quaternary ammonium compounds (QACs), benzalkonium chloride, cetylpyridinium, and chlorhexidine. Polysorbate 80 (5 g/L) neutralises phenolics, hexachlorophene, parabens, and formalin. Together they cover the most common disinfectant classes used in cosmetics and pharmaceutical manufacturing environments.
❓ Can I use this for ISO 21149 cosmetics microbiology?
Yes. AS-1351 is the plating medium referenced in ISO 21149:2017 for enumeration of aerobic mesophilic bacteria in cosmetics. After enrichment in a neutralising broth (AS-1433 or Letheen Broth, AS-1271), plate onto AS-1351 and incubate at 30–35°C for 48–72h.
Cross-Reference / Equivalents
| Manufacturer | Product Name | Cat. No. |
|---|---|---|
| Merck Millipore | Caso Broth with Lecithin and Polysorbate 80 | 1.10692 |
| Oxoid (Thermo Fisher) | Tryptone Soya Broth with Lecithin and Polysorbate 80 | CM0693 |
| BD Difco | Tryptic Soy Broth with Lecithin and Polysorbate 80 | 254280 |
| HiMedia | Casein Lecithin Polysorbate Broth | M1242 |
AuSaMicS AS-1351 — manufactured in Melbourne, same-week dispatch, no import delays, full documentation included.
Related Products
Product Specifications
| Product Name | Tryptic Soy Agar with Lecithin and Polysorbate 80 |
| Catalogue Number | AS-1433 |
| Synonyms | SCDLP80 Broth; MCT Broth; Microbial Content Test Broth; Tryptone Soya Broth with Lecithin and Tween 80; CASO Broth with Lecithin and Polysorbate 80 |
| Medium Type | Non-selective neutralising liquid enrichment medium (broth) |
| Agar Counterpart | AS-1351 — Tryptic Soy Agar with Lecithin and Polysorbate 80 |
| Dissolution | ~31 g/L in distilled water |
| Final pH at 25°C | 7.3 ± 0.2 |
| Sterilisation | Autoclave 121°C for 15 minutes |
| Incubation | 30–35°C / 48–72h (bacteria); 20–25°C / 5–7 days (yeast & mould) |
| Appearance (powder) | Cream to light beige, free-flowing homogeneous powder |
| Appearance (prepared broth) | Clear to slightly opalescent, light amber; slight turbidity from lecithin is normal |
| Storage (powder) | 15–25°C, tightly sealed, away from moisture and direct sunlight |
| Storage (prepared broth) | 2–8°C; use within 4 weeks. Discard if turbid prior to inoculation. |
| HS Tariff Code | 3821.00.00 |
Quality Control Organisms
| Organism (ATCC) | Inoculum (CFU) | Incubation | Recovery |
|---|---|---|---|
| Staphylococcus aureus (6538) | ≤100 | 35°C / 24–48h | Good ✓ |
| Escherichia coli (8739) | ≤100 | 35°C / 24–48h | Good ✓ |
| Pseudomonas aeruginosa (9027) | ≤100 | 35°C / 24–48h | Good ✓ |
| Candida albicans (10231) | ≤100 | 25°C / 48–72h | Good ✓ |
| Aspergillus brasiliensis (16404) | ≤100 | 25°C / 5–7 days | Good ✓ |
Applicable Standards
| ISO 21149:2017 | Cosmetics — Enumeration of aerobic mesophilic bacteria (plating medium) |
| ISO 21150:2015 | Cosmetics — Detection of E. coli |
| ISO 18415:2017 | Cosmetics — Detection of specified and non-specified microorganisms |
| ISO 11133:2014 | Culture media performance testing |
| USP <61> / EP 2.6.12 | Microbiological examination of non-sterile products |
| FDA BAM | Bacteriological Analytical Manual — Cosmetics |
Literature & References
| # | Reference |
|---|---|
| 1 | Merck Microbiology Manual, 12th Ed. CASO Agar with Lecithin and Polysorbate 80, Cat. 1.05463. Merck KGaA; 2010. [Confirmed formula: Casein 15g, Soy 5g, NaCl 5g, Lecithin 0.7g, Polysorbate 80 5g, Agar 15g, pH 7.3] |
| 2 | ISO 21149:2017. Cosmetics — Microbiology — Enumeration and detection of aerobic mesophilic bacteria. Geneva: ISO; 2017. |
| 3 | ISO 11133:2014. Preparation, production, storage and performance testing of culture media. Geneva: ISO; 2014. |
| 4 | Dima S, et al. Evaluation of neutralising agents in microbiological testing of cosmetics. J Appl Microbiol. 2007;102(5):1434–1443. |
📄 Full SDS available: 16-section GHS SDS (Australian WHS Regulations 2023 / GHS 7th Edition) — support@ausamics.com.au
Hazard Identification (GHS 7th Ed / WHS 2023)
| GHS Classification | NOT classified as hazardous under Australian WHS Regulations 2023. No hazard statements required. |
| Other Hazards | Combustible powder (dust); Polysorbate 80 is hygroscopic — reseal promptly after use. |
| Signal Word | None required |
| Emergency | Poisons Information Centre: 13 11 26 (24 hr, Australia) |
PPE & First Aid Summary
| Respiratory | P1 particulate filter when weighing bulk powder |
| Eye Protection | Safety glasses when handling bulk powder or prepared broth |
| Skin | Nitrile gloves (min 0.11 mm); lab coat |
| Skin Contact | Wash with soap and water ≥15 min. No significant hazard expected. |
| Eye Contact | Irrigate with water ≥15 min. Seek medical attention if irritation persists. |
| Disposal | Autoclave inoculated plates (121°C / 15 min) before disposal as biological waste. Unused powder: general chemical waste. |
| Transport | Not dangerous goods — ADG, IMDG, IATA |
Composition (for reference)
| Component | g/L | CAS | Hazard |
|---|---|---|---|
| Casein Peptone | 15.0 | 91079-38-8 | Not hazardous |
| Soy Peptone | 5.0 | 68153-28-6 | Not hazardous |
| Sodium Chloride | 5.0 | 7647-14-5 | Not hazardous |
| Lecithin (Soya) | 0.7 | 8002-43-5 | Not hazardous |
| Polysorbate 80 | 5.0 | 9005-65-6 | Not hazardous at use level |
| Agar | 15.0 | 9002-18-0 | Not hazardous |
Quality Specifications
| Parameter | Specification | Method |
|---|---|---|
| Appearance (powder) | Cream to light beige, free-flowing, homogeneous | Visual |
| Appearance (prepared) | Clear to slightly opalescent, light amber; slight turbidity normal | Visual |
| pH (prepared, 25°C) | 7.3 ± 0.2 | pH meter |
| pH (prepared, 25°C) | 7.3 ± 0.2 | Visual / Gelometer |
| Moisture (powder) | ≤5.0% (w/w) | Loss on drying |
| Neutralisation — QAC | Growth in 0.1% benzalkonium chloride challenge | Plate assay |
| Neutralisation — Phenolic | Growth in 0.5% phenol challenge | Plate assay |
| Growth promotion | Good growth of all 5 QC organisms (ISO 11133:2014) | Inoculation test |
| Batch COA | Available for every production lot | On request |
Documentation Available
| 📄 Certificate of Analysis (COA) | Batch-specific | Included with every order |
| 📋 Technical Data Sheet (TDS) | Full specifications, formula, preparation protocol |
| ⚠️ Safety Data Sheet (SDS) | GHS 7th Ed | Australian WHS 2023 | 16 sections |
⚠️ For laboratory and industrial QC use only. Not for food, cosmetic, therapeutic, or household use.