SPS Agar (Sulfite Polymyxin Sulfadiazine Agar) | Ausamics

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SPS Agar; Sulfite Polymyxin Sulfadiazine Agar

AuSaMicS Life Science™ | www.ausamics.com.au

Product Description

Cat. No. AS-1353

For Laboratory Use Only

Catalog Number

AS-1353

Medium Type

Selective Differential Agar

Target Organism

Clostridium perfringens

Final pH

7.3 ± 0.2

Overview

SPS Agar (Sulfite Polymyxin Sulfadiazine Agar) is a selective medium designed for the isolation, enumeration, and differentiation of Clostridium perfringens from food, water, and environmental samples. The combination of sulfite, polymyxin B, and sulfadiazine selectively inhibits the growth of competing bacteria while promoting the development of C. perfringens, which reduces sulfite to form black colonies for easy visual detection.

SPS Agar is widely used in food safety testing, environmental monitoring, and microbiological quality control where selective recovery of sulfite-reducing anaerobes is required.

Key Features & Benefits

🎯

Highly Selective Sulfite, polymyxin B, and sulfadiazine inhibit non-target organisms and support target recovery.

⚫

Differential Detection Black colonies indicate sulfite reduction by C. perfringens, enabling simple visual differentiation.

🫙

Supports Anaerobic Growth Optimized nutrient system for cultivation of obligate anaerobic, sulfite-reducing organisms.

📏

Compliance-Oriented Suitable for use in recognized food, water, and environmental microbiology workflows.

Applications

🍽️

Food & Water Microbiology

Isolation and enumeration of Clostridium perfringens from food and water samples
Food microbiology and safety testing
Routine microbiological quality control

🌍

Environmental Monitoring

Monitoring of anaerobic, sulfite-reducing bacteria
Environmental surveillance in production facilities
Quality control in water and food production environments

Composition (per Liter)

Component	Concentration	Function
Proteose Peptone / Peptone	15 g	Nitrogen and nutrient source
Yeast Extract	3 g	Vitamin and growth factor source
Sodium Sulfite	1 g	Differential sulfite reduction system
Dextrose	2 g	Fermentable carbohydrate source
Agar	15 g	Solidifying agent
Polymyxin B	1000 units	Selective agent
Sulfadiazine	100 mg	Selective inhibitor
Purified Water	q.s. to 1 L	Solvent / medium base

Composition & Properties

Medium FunctionIsolation, enumeration, and differentiation of Clostridium perfringens Differential ReactionBlack colony formation due to sulfite reduction Growth ConditionsSupports anaerobic growth of target organisms Final pH7.3 ± 0.2 at 25 °C

Preparation

Step	Procedure
1	Suspend the medium in purified water and heat to dissolve completely.
2	Dispense into plates or tubes as required.
3	Sterilize by autoclaving at 121 °C for 15 minutes.
4	Cool and pour plates or allow tubes to solidify before use.

Incubation & Interpretation

Temperature37 °C Duration18–24 hours; up to 48 hours for slow-growing strains Positive ResultBlack colonies indicating sulfite-reducing C. perfringens Negative ResultNo growth or non-black colonies

Storage

Powdered Medium15–30 °C, dry and tightly sealed Prepared Plates / Tubes2–8 °C, protected from light Shelf LifeRefer to product label for optimal performance

⚠ Warning For laboratory use only. Not for human, animal, or food consumption.

Disclaimer This product is supplied for laboratory and industrial microbiology use only. Performance depends on correct preparation, sterilization, storage, incubation, and anaerobic handling conditions. End users are responsible for validating suitability for their intended application and quality system.

Safety Data Sheet

SPS Agar; Sulfite Polymyxin Sulfadiazine Agar | Cat. AS-1353

SDS — GHS Format

SDS-AS-1353 | Rev. 1.0

Issue Date: March 2026

Section 1 — Identification

Product NameSPS Agar; Sulfite Polymyxin Sulfadiazine Agar Catalog No.AS-1353 Recommended UseIsolation, enumeration, and differentiation of Clostridium perfringens in laboratory and industrial microbiology SupplierAuSaMicS Pty Ltd, 31 Longview CT, Thomastown VIC 3074, Australia Emergency Contact+61 412 520 598 | support@ausamics.com.au Emergency ServicesAustralia: 000 | Poisons Information Centre: 13 11 26

Section 2 — Hazard Identification

GHS Classification: Not generally classified as hazardous under normal laboratory use. Powder may cause mild irritation to eyes, skin, or respiratory tract if dust is generated. Contains selective agents intended for microbiological use only.

Signal WordNone required Hazard StatementsNot generally classified as hazardous. Avoid dust formation and unnecessary contact with eyes, skin, and respiratory tract. Precautionary StatementsUse good laboratory practice. Wear suitable gloves, eye protection, and laboratory coat when handling bulk powder. Regulatory StatusFor laboratory use only. Not intended for human, animal, or food consumption.

Section 3 — Composition / Ingredients

Component	Typical Content	Function
Proteose Peptone / Peptone	15 g/L	Nutrient base
Yeast Extract	3 g/L	Growth factor source
Sodium Sulfite	1 g/L	Differential sulfite reduction component
Dextrose	2 g/L	Carbohydrate source
Agar	15 g/L	Solidifying agent
Polymyxin B	1000 units/L	Selective agent
Sulfadiazine	100 mg/L	Selective inhibitor
Purified Water	q.s. to 1 L	Solvent / medium base

Section 4 — First Aid Measures

Skin ContactWash with soap and water. Seek medical advice if irritation develops. Eye ContactRinse cautiously with water for several minutes. Seek medical advice if irritation persists. InhalationMove to fresh air if dust is inhaled. Seek medical advice if symptoms persist. IngestionRinse mouth with water. Seek medical advice if discomfort occurs.

Section 5 — Fire-Fighting Measures

Suitable Extinguishing MediaWater spray, foam, dry chemical, or carbon dioxide Specific HazardsCombustible organic components may produce smoke and carbon oxides when heated strongly Protective EquipmentUse standard fire-fighting PPE and respiratory protection where required

Section 6 — Accidental Release Measures

Personal PrecautionsAvoid generating dust. Wear gloves and eye protection during cleanup. Environmental PrecautionsPrevent bulk release to drains and waterways. Clean-Up MethodsSweep or vacuum into a suitable container. Wipe remaining residue with a damp cloth.

Sections 7–16 — Handling, Storage, PPE, Physical, Stability, Disposal, Transport, Regulatory

HandlingUse good laboratory practices. Avoid inhalation of dust and unnecessary contact with eyes or skin. StoragePowdered medium: 15–30 °C, dry and tightly sealed. Prepared medium: 2–8 °C, protected from light. PPEGloves, safety glasses, and laboratory coat recommended for bulk handling. AppearanceDehydrated powder for microbiological medium preparation Final pH7.3 ± 0.2 at 25 °C StabilityStable under recommended storage conditions DisposalDispose according to local laboratory and environmental regulations TransportTypically not classified as dangerous goods Prepared ByAuSaMicS Pty Ltd — Quality & Regulatory Affairs

Disclaimer This SDS-style content is provided in website format and should be aligned with your approved internal or regulatory safety documentation before publication where required.

Certificate of Analysis

SPS Agar; Sulfite Polymyxin Sulfadiazine Agar | Cat. AS-1353

COA — Batch Specific

COA-AS-1353-L001

Issue Date: March 2026

Product Information

Product NameSPS Agar; Sulfite Polymyxin Sulfadiazine Agar Catalog No.AS-1353 Medium TypeSelective differential agar Target OrganismClostridium perfringens Final pH7.3 ± 0.2 at 25 °C ApplicationIsolation, enumeration, and differentiation of sulfite-reducing anaerobes in food, water, and environmental microbiology

Lot / Batch Information

Lot NumberAS1353-L001 Manufacture DateMarch 2026 Expiry DateMarch 2029 Storage15–30 °C, dry and tightly sealed AppearanceConforms ✓ Pack SizeTBD

Analytical Test Results

No.	Test / Criteria	Method	Specification / Limit	Result	Status
1	Appearance	Visual inspection	Homogeneous dehydrated powder	Conforms	PASS
2	Colour of Medium	Visual inspection	Conforms to standard	Conforms	PASS
3	pH (prepared medium)	Potentiometry	7.3 ± 0.2 at 25 °C	Conforms	PASS
4	Gel Strength / Solidification	Preparation test	Firm gel after sterilization	Conforms	PASS
5	Selectivity	Biological performance test	Inhibits non-target organisms as specified	Conforms	PASS
6	Differential Performance	Biological performance test	Supports black colony development for sulfite-reducing target organisms	Conforms	PASS
7	Sterility Check	Incubation / QC	No contamination in uninoculated control	Conforms	PASS

Overall Batch Disposition

RELEASED — COMPLIES WITH SPECIFICATIONS

Authorisation

Analysed By

QC Analyst | AuSaMicS Pty Ltd

Date: _______________

Approved & Released By

Quality Manager | AuSaMicS Pty Ltd