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TETRATHIONATE BROTH BASE (AS‑1359) (Ausamics Life Science — Selective Salmonella Enrichment)

TETRATHIONATE BROTH BASE (AS‑1359) (Ausamics Life Science — Selective Salmonella Enrichment)

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AuSaMicS Life Science • Salmonella Enrichment

Tetrathionate Broth Base

Selective Enrichment Medium for Salmonella Detection

Tetrathionate Broth Base is a highly selective enrichment medium formulated to recover Salmonella from food, water, and clinical specimens. Tetrathionate and brilliant green suppress competing flora, enabling the multiplication of low-level or stressed Salmonella in mixed microbial populations.

AS‑1359 🧪 Selective Enrichment ✓ Australian Stock 🦠 Salmonella Recovery
🚀 Same‑Day Dispatch 🥚 Food & Water Testing 📚 ISO • FDA‑BAM • WHO

🏆 Salmonella Enrichment Specialist

🎯 Tetrathionate Selectivity – suppresses competing flora
🧪 Optimized Nutrients – supports Salmonella multiplication
📋 ISO & FDA‑BAM Compliant
Reliable Recovery – even in mixed samples
📞 Expert Support
Brilliant Green Indicator
Enhances selectivity against Gram‑positive and coliform bacteria

📘 Overview & Applications

Tetrathionate Broth Base is a selective enrichment medium used to isolate and recover Salmonella spp. from food, water, and clinical specimens. Tetrathionate and brilliant green inhibit competing organisms, increasing the likelihood of detecting low-level Salmonella in mixed samples.

Widely used in food microbiology, clinical diagnostics, and environmental monitoring.

🎯 Applications
  • • Enrichment of Salmonella from food & water
  • • ISO, FDA‑BAM, WHO compliant workflows
  • • Pre‑enrichment before XLD, SS, BGA
  • • Quality control in food processing
🔍 Key Features
  • • Tetrathionate selectively inhibits non‑target flora
  • • Supports rapid Salmonella multiplication
  • • Compatible with downstream selective plating
  • • Standardized for global compliance

🧪 Complete Tetrathionate Formulation

📋 Composition (per Liter)

Component Amount Function
Peptone / Proteose Peptone 10 g Nitrogen & growth factors
Yeast Extract 5 g Vitamins & cofactors
Sodium Chloride 5 g Osmotic balance
Sodium Tetrathionate 10 g Selective agent
Brilliant Green 0.01 g Suppresses Gram‑positive & coliforms

Final pH: 7.2 ± 0.2 at 25 °C

⚗️ Preparation, Incubation & Interpretation

🧴 Preparation

  1. Suspend medium in purified water.
  2. Heat with agitation until fully dissolved.
  3. Dispense into tubes or flasks.
  4. Sterilize at 121 °C for 15 minutes.
  5. Cool before use.

🌡️ Incubation

  • Temperature: 35–37 °C
  • Duration: 18–24 hours
  • Up to 48 hours for slow-growing Salmonella

🔍 Interpretation

  • Positive enrichment: turbidity
  • Subculture onto XLD, SS, or other selective agar
  • No growth suggests absence or suppression of Salmonella

🔬 Quality Control & Standards

Positive Control:
Salmonella Typhimurium
Expected: growth/turbidity
Negative Control:
E. coli
Expected: inhibited or minimal growth
Standards:
ISO • FDA‑BAM • WHO
Documentation:
COA + QC data available

📦 Product Snapshot

Product Name Tetrathionate Broth Base
Catalog Number AS‑1359
Format Dehydrated culture medium
Use Selective enrichment of Salmonella
Compliance ISO • FDA‑BAM • WHO

🧊 Storage & Stability

  • Powdered medium: 15–30 °C, dry & sealed
  • Prepared medium: 2–8 °C, protected from light
  • Shelf life: Use within recommended period

⚠️ Warnings & Precautions

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