TETRATHIONATE BROTH BASE (AS‑1359) (Ausamics Life Science — Selective Salmonella Enrichment)
Tetrathionate Broth Base
Selective Enrichment Medium for Salmonella Detection
Tetrathionate Broth Base is a highly selective enrichment medium formulated to recover Salmonella from food, water, and clinical specimens. Tetrathionate and brilliant green suppress competing flora, enabling the multiplication of low-level or stressed Salmonella in mixed microbial populations.
🏆 Salmonella Enrichment Specialist
📘 Overview & Applications
Tetrathionate Broth Base is a selective enrichment medium used to isolate and recover Salmonella spp. from food, water, and clinical specimens. Tetrathionate and brilliant green inhibit competing organisms, increasing the likelihood of detecting low-level Salmonella in mixed samples.
Widely used in food microbiology, clinical diagnostics, and environmental monitoring.
- • Enrichment of Salmonella from food & water
- • ISO, FDA‑BAM, WHO compliant workflows
- • Pre‑enrichment before XLD, SS, BGA
- • Quality control in food processing
- • Tetrathionate selectively inhibits non‑target flora
- • Supports rapid Salmonella multiplication
- • Compatible with downstream selective plating
- • Standardized for global compliance
🧪 Complete Tetrathionate Formulation
📋 Composition (per Liter)
Final pH: 7.2 ± 0.2 at 25 °C
⚗️ Preparation, Incubation & Interpretation
🧴 Preparation
- Suspend medium in purified water.
- Heat with agitation until fully dissolved.
- Dispense into tubes or flasks.
- Sterilize at 121 °C for 15 minutes.
- Cool before use.
🌡️ Incubation
- Temperature: 35–37 °C
- Duration: 18–24 hours
- Up to 48 hours for slow-growing Salmonella
🔍 Interpretation
- Positive enrichment: turbidity
- Subculture onto XLD, SS, or other selective agar
- No growth suggests absence or suppression of Salmonella
🔬 Quality Control & Standards
Salmonella Typhimurium
Expected: growth/turbidity
E. coli
Expected: inhibited or minimal growth
ISO • FDA‑BAM • WHO
COA + QC data available
📦 Product Snapshot
| Product Name | Tetrathionate Broth Base |
| Catalog Number | AS‑1359 |
| Format | Dehydrated culture medium |
| Use | Selective enrichment of Salmonella |
| Compliance | ISO • FDA‑BAM • WHO |
🧊 Storage & Stability
- Powdered medium: 15–30 °C, dry & sealed
- Prepared medium: 2–8 °C, protected from light
- Shelf life: Use within recommended period