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TGE Agar | AS-1360 | Tryptone Glucose Extract Agar | AuSaMicS
$39.00 AUD
AuSaMicS Life Science · General-Purpose Culture Medium
TGE Agar
Tryptone Glucose Extract Agar · AS-1360
A nutrient-rich, non-selective solid medium for broad-spectrum cultivation, enumeration, and maintenance of aerobic heterotrophic bacteria and fungi. Trusted in food microbiology, environmental monitoring, QC laboratories, and research.
Non-selective General Purpose Australian Made In Stock
Product Snapshot
Catalogue No.AS-1360
FormatDehydrated powder
Reconstitution37 g / L
Final pH (25 °C)7.2 ± 0.2
Sterilisation121 °C / 15 min
Incubation30–37 °C · 18–48 h
SelectivityNone
DocumentationCOA / TDS / SDS
Overview
TGE Agar is a classical, general-purpose solid culture medium that supports robust growth of a wide variety of aerobic heterotrophic bacteria and fungi. By combining tryptone, yeast extract, and glucose, it delivers all essential nutrients — nitrogen, vitamins, carbon — without imposing any selective pressure, making it the benchmark choice for total aerobic plate counts (APC), culture maintenance, and environmental monitoring.
Primary Use
- Aerobic plate count (APC)
- Culture maintenance
- Environmental monitoring
- Food & water QC
Industries
- Food & beverage
- Pharmaceutical QC
- Clinical / research
- Education
Key Strengths
- Non-selective, versatile
- Reproducible results
- Plates, slants, pours
- Full AuSaMicS docs
Ingredients & Function
Total dry weight ≈ 37 g/L · Final pH 7.2 ± 0.2 at 25 °C
Preparation Protocol
Incubation temp.30–37 °C
Duration18–48 h (up to 72 h for slow growers)
AtmosphereAerobic (standard incubator)
Expected resultVisible colonies; no differential readout
Colony appearanceVariable; organism-dependent
Plate storage2–8 °C up to 4 weeks (aseptic)
Comparative Media & Workflow Position
Advantages & Limitations
Advantages
- Broadest taxonomic recovery — bacteria and fungi
- Versatile formats: plates, slants, pour plates
- Reproducible for routine APC testing
- Supports fastidious organisms via yeast extract vitamins
- Simple preparation — single autoclave step
- Full GHS documentation (COA, TDS, SDS) available
Limitations
- No selectivity — unsuitable for targeted pathogen isolation
- No differential indicators — all colonies appear similar
- Overgrowth on high-load samples requires dilution
- Anaerobic organisms require modified incubation
- Not appropriate for strict anaerobe enumeration
Cross-Reference / Equivalent Products
Related AuSaMicS Products
Storage
Powder15–30 °C, dry, sealed
Prepared2–8 °C, dark
Shelf lifePer label expiry
Post-prepUp to 4 weeks (aseptic)
AuSaMicS Advantage
- Manufactured in Melbourne, VIC
- Same-week dispatch
- Full GHS-compliant docs
- Direct technical support
- ABN: 56 676 640 467
For laboratory use only. Not for human, animal, or food consumption. Follow standard biosafety precautions when handling cultures.
Contact & Support
Mode of Action
TGE Agar supports broad-spectrum microbial growth by supplying all three macronutrient classes. Tryptone furnishes nitrogen as amino acids and short peptides from enzymatic casein hydrolysis. Yeast extract contributes B-vitamins, nucleotide precursors, and trace minerals that accelerate biosynthetic pathways. Glucose provides an immediately catabolisable carbon and energy source via glycolysis and the TCA cycle. Sodium chloride maintains isotonic osmolarity, and agar provides a stable, biologically inert solid matrix.
Physical & Chemical Specifications
Preparation Parameters
Literature & References
1. Reasoner, D.J. & Geldreich, E.E. (1985). A new medium for the enumeration and subculture of bacteria from potable water. Applied and Environmental Microbiology, 49(1), 1–7.
2. ISO 11133:2014. Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media.
3. Bridson, E.Y. (Ed.). (1998). The Oxoid Manual, 8th ed. Unipath Ltd., Basingstoke, UK.
4. APHA (2017). Standard Methods for the Examination of Water and Wastewater, 23rd ed. Method 9215B — Heterotrophic Plate Count.
Ordering Info
SKUAS-1360
Pack sizes100 g · 500 g · 2 kg
DocumentsCOA · TDS · SDS · PD
DispatchMelbourne, same week
Limitations
- Non-selective — use selective media when targeting specific pathogens
- Anaerobes require modified atmosphere
- Dilute heavily contaminated samples before plating
- Do not use beyond label expiry
Performance Testing — Quality Control
PDR = Percent Difference in Recovery vs reference medium. Inoculum ≤ 100 CFU per strain per ISO 11133 guidance.
Release Criteria
Testing Standard
All AuSaMicS culture media are performance-tested in accordance with ISO 11133:2014 prior to release.
Each batch is assigned a unique lot number traceable to raw material records. Certificate of Analysis (COA) available on request or via download on the product page.
Hazard Summary
GHS Classification: Not classified as hazardous under GHS / WHS Regulations (Australia).
Signal Word: None
Hazard Statements: None applicable
Precautionary Measures: Avoid inhalation of dust during weighing. Wear appropriate laboratory coat and gloves. Wash hands after handling. For laboratory use only.
GHS Sections Summary
Full 16-section GHS-compliant Safety Data Sheet (SDS) is available as a downloadable PDF from the AuSaMicS product page or upon request. Contact support@ausamics.com.au for batch-specific documentation.
DISCLAIMER: This product is intended for professional laboratory use only by trained personnel. AuSaMicS Pty Ltd makes no representations regarding fitness for a particular purpose beyond the stated intended use. Users bear full responsibility for verifying suitability for their specific application. AuSaMicS Pty Ltd shall not be liable for any direct, indirect, or consequential damages arising from misuse or misapplication. © AuSaMicS Pty Ltd. All rights reserved. ABN: 56 676 640 467.