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Thayer-Martin Medium Base Selective Neisseria Isolation Medium
  • Thayer-Martin Medium Base Selective Neisseria Isolation Medium
Thayer-Martin Medium Base

Thayer-Martin Medium Base

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AuSaMicS Life Science • Selective Pathogen Isolation

Thayer-Martin Medium Base

Selective Neisseria Isolation Medium

Gold-standard selective medium for recovery of pathogenic Neisseria species from clinical specimens. Enhanced formulation provides superior growth support for fastidious organisms while suppressing contaminating flora.

AS-1361 🧪 Selective Base ✓ Australian Stock 🦠 Pathogen Grade
Same-Day Dispatch: Australian Stock STI Diagnostics: N. gonorrhoeae specialist VCNT Ready: Antibiotic supplement compatible

 Pathogen Isolation Specialist

High Recovery - N. gonorrhoeae optimized
Selective Power - VCNT antibiotic base
Complete Package - COA + Protocols
Fast Supply - Ships within 24h
Expert Support - Clinical guidance
Starch Protection
Neutralizes fatty acid toxicity

 Complete Thayer-Martin Formulation

📋 Base Medium Composition (per Liter)

Ingredient Concentration Primary Function Clinical Benefit
Proteose Peptone No. 3 15.0 g Rich nitrogen source High amino acid content for fastidious growth
Beef Extract 3.0 g Growth factors Essential vitamins, minerals, nucleotides
Yeast Extract 3.0 g B-vitamin complex Supports metabolic requirements
Corn Starch 1.0 g Fatty acid neutralizer Prevents toxic fatty acid inhibition
Sodium Chloride 5.0 g Osmotic balance Maintains cellular integrity
Bacteriological Agar 12.0 g Solidifying agent Optimal gel strength for colonies

Essential Supplement System

Hemoglobin (5%)
50ml/L - Iron source for heme-requiring Neisseria
IsoVitaleX Enrichment
10ml/L - Growth factors, coenzymes, vitamins
VCNT Antibiotic Supplement (per Liter):
Vancomycin: 3 mg
Colistin: 7.5 mg
Nystatin: 12,500 units
Trimethoprim: 5 mg
Complete Medium: Base + Hemoglobin + IsoVitaleX + VCNT = Modified Thayer-Martin (MTM) for selective N. gonorrhoeae isolation

Complete Neisseria Isolation Media Portfolio

Medium Selectivity Level Target Organisms Inhibited Flora Clinical Application Key Advantage
Thayer-Martin (MTM) (AS-1361) 🔴🔴🔴 High N. gonorrhoeae, N. meningitidis Gram+, most Gram-, yeast, Proteus STI diagnosis, CSF cultures Gold standard selectivity
Chocolate Agar (AS-1172) ⚪ None All Neisseria, Haemophilus None (non-selective) Primary isolation, fastidious organisms Maximum recovery
GC-Lect Agar 🟡🟡 Moderate N. gonorrhoeae (lecithinase+) Lecithinase-negative bacteria Confirmatory testing Lecithinase differentiation
Martin-Lewis Agar Base (AS-1423) 🔴🔴🔴🔴 Very High N. gonorrhoeae Enhanced Proteus inhibition Heavily contaminated specimens Maximum selectivity
NYC Medium Base (AS-1424) 🟡🟡 Moderate N. gonorrhoeae, some enterics Gram-positive bacteria Rectal specimens Allows some enterics
Transgrow Medium Base (AS-1425) 🟢🟢 Low-Moderate N. gonorrhoeae Some contaminants Transport medium CO₂ atmosphere included

 Complete N. gonorrhoeae Detection Workflow

 Primary Isolation Methods

Method Medium Specimen Type Recovery Rate
Modified Thayer-Martin Base + VCNT supplements Urogenital, rectal, pharyngeal 90-95%
Chocolate Agar Non-selective enriched CSF, blood, sterile sites 95-98%
Martin-Lewis Enhanced selectivity Heavily contaminated 85-90%
GC-Lect Lecithinase differential Confirmatory testing 80-85%

 Essential Confirmatory Tests

1. Gram Stain
Gram-negative diplococci
AS-GramKit →
2. Oxidase Test
Positive reaction (purple)
AS-Oxidase →
3. Catalase Test
Positive (bubbling)
AS-Catalase →
4. Carbohydrate Tests
Glucose+, others variable
AS-CarbKit →
Molecular Confirmation: PCR/NAAT testing recommended for definitive identification and antimicrobial resistance detection

 Strategic Medium Selection for Neisseria Isolation

✅ Thayer-Martin Advantages

  • ✓ Gold Standard: Internationally recognized method
  • ✓ Optimal Selectivity: VCNT antibiotic system
  • ✓ High Recovery: 90-95% N. gonorrhoeae detection
  • ✓ Contamination Control: Suppresses interfering flora
  • ✓ Versatile Application: Multiple specimen types
  • ✓ Quality Assured: Standardized formulation

⚠️ Method Considerations

  • ▲ Complex Preparation: Multiple supplements required
  • ▲ Cost Factor: Expensive antibiotics (VCNT)
  • ▲ Storage Sensitive: Prepared plates limited shelf life
  • ▲ Antibiotic Resistance: Some strains may be inhibited
  • ▲ Temperature Critical: CO₂ incubation required

🎯 Clinical Selection Guide

  • ◆ STI Clinics: Thayer-Martin (routine screening)
  • ◆ Emergency Dept: Chocolate + Thayer-Martin
  • ◆ Sterile Sites: Chocolate Agar preferred
  • ◆ Heavy Contamination: Martin-Lewis
  • ◆ Transport/Field: Transgrow medium

📊 Specimen-Specific Performance

Specimen Type Thayer-Martin Chocolate Agar Recommendation
Urethral Swabs Excellent Good Thayer-Martin preferred
Cervical Swabs Excellent Good Thayer-Martin preferred
Rectal Swabs Excellent Fair Thayer-Martin essential
Pharyngeal Swabs Good Fair Both media recommended
CSF Good Excellent Chocolate Agar preferred
Joint Fluid Good Excellent Both media optimal

🔬 Quality Control & Performance Standards

Positive Control:
N. gonorrhoeae ATCC 43069
Good growth on MTM
Inhibition Control:
E. coli ATCC 25922
Complete inhibition
Selectivity Test:
S. epidermidis ATCC 12228
No growth
Physical Properties:
pH 7.2-7.6
Clear, brown colored plates
Standards Compliance: Meets Clinical and Laboratory Standards Institute (CLSI) guidelines for Neisseria gonorrhoeae susceptibility testing and isolation media

📋 Technical Specifications

Catalogue Number AS-1361
Dehydrated Weight 39.0 g/L
Final pH 7.3 ± 0.2 (at 25°C)
Appearance Light tan powder
Sterilization 121°C, 15 minutes
Grade Clinical Grade
Incubation 35-37°C, 5-10% CO₂
Shelf Life 3 years

 Target Pathogens

  • ✓ Neisseria gonorrhoeae - Primary target
  • ✓ Neisseria meningitidis - Secondary target
  • ✓ Pathogenic Neisseria - Selective recovery
  • ❌ Commensal Neisseria - Reduced growth
  • ❌ Gram+ bacteria - Vancomycin inhibition

 Clinical Applications

  • STI Testing: Gonorrhea diagnosis
  • Primary Isolation: Clinical specimens
  • Research: Neisseria studies
  • Susceptibility: Antibiotic testing base
  • Public Health: Surveillance programs

 Complete Preparation

  1. 1 Suspend 39g base in 1L water
  2. 2 Heat and autoclave 121°C/15min
  3. 3 Cool to 50°C, add supplements
  4. 4 Pour plates, incubate 35°C + CO₂

 Storage & Handling

  • ✓ Dehydrated: 15-30°C, dry storage
  • ✓ Prepared plates: 2-8°C, up to 1 week
  • ⚠️ Light sensitive: Dark storage required
  • 🔒 Supplements: Freeze VCNT antibiotics

🧪 Complete Neisseria Isolation System

Selective Isolation Media

Chocolate Agar

Non-selective fastidious

View Product
Martin-Lewis Agar

Enhanced selectivity

View Product
GC-Lect Agar

Lecithinase differential

View Product
NYC Medium

Rectal specimen use

View Product

Essential Medium Supplements

VCNT Antibiotics

Selective supplement

View Product
Hemoglobin Solution

Iron source (5%)

View Product
IsoVitaleX Enrichment

Growth factor complex

View Product
CO₂ Tablets

Atmosphere generation

View Product

Confirmatory Testing Reagents

Gram Stain Kit

Primary identification

View Product
Oxidase Reagent

Essential confirmatory

View Product
Catalase Reagent

Secondary confirmation

View Product
Carbohydrate Tests

Species differentiation

View Product

Need Pathogen Isolation Support?

Our pathogen isolation specialists can help optimize your Neisseria testing protocols and supplement preparation

For clinical laboratory and research use only. Not intended for food, feed, household, cosmetic, therapeutic, or personal use.
AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
Same-day dispatch • Australian stock • Clinical grade • CLSI protocols included

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