Skip to product information
Request a Quote
Thioglycollate Medium, Brewer Modified | USP Sterility Testing Medium for Aerobic & Anaerobic Bacteria
$45.00 AUD
Thioglycollate Medium, Brewer Modified
Enriched Reducing Medium for Anaerobic, Microaerophilic, and Pharmaceutical Sterility Testing
Catalogue No: AS-1364
Enriched Reducing Medium for Anaerobic, Microaerophilic, and Pharmaceutical Sterility Testing
Catalogue No: AS-1364
Anaerobic Culture
USP <71> Compliant
BP / EP Compliant
Sterility Testing
Resazurin Indicator
Australian Made
Overview
Thioglycollate Medium, Brewer Modified is an enriched, reducing culture medium designed for the cultivation of obligate anaerobes, microaerophiles, and facultative anaerobes. The Brewer modification provides a dual reducing system — sodium thioglycollate and L-cystine — creating a sufficiently low oxidation-reduction potential to support strict anaerobes without requiring anaerobic jars in routine tube culture.
The medium incorporates resazurin as a visual redox indicator, allowing real-time monitoring of oxygen penetration depth. The semi-solid agar content (0.5 g/L) retards convection and maintains an oxygen gradient. Meets requirements for Fluid Thioglycollate Medium per USP <71>, BP 2023, and EP 2.6.1.
Principle of the Medium
| Sodium Thioglycollate (0.5 g/L) | Primary reducing agent — scavenges dissolved oxygen, lowers Eh to support obligate anaerobes |
| L-Cystine (0.5 g/L) | Secondary reducing agent and sulfur source — enhances reducing capacity |
| Resazurin (0.001 g/L) | Redox indicator — colourless (anaerobic) ↔ pink (oxygen penetration) |
| Agar (0.5 g/L) | Semi-solid — retards convection, maintains oxygen gradient for growth zone interpretation |
| Casein Digest + Yeast Extract | Rich nitrogen, vitamins, and growth factors for fastidious anaerobes |
| Dextrose (2.5 g/L) | Fermentable carbon source and mild reductant |
Typical Composition (per litre)
| Ingredient | Amount (g/L) | Function |
|---|---|---|
| Pancreatic Digest of Casein | 15.0 | Nitrogen, peptides, amino acids |
| Yeast Extract | 5.0 | Vitamins, growth factors |
| Dextrose (D-Glucose) | 2.5 | Carbon source, mild reductant |
| Sodium Chloride | 5.0 | Osmotic balance |
| Sodium Thioglycollate | 0.5 | Primary reducing agent |
| L-Cystine | 0.5 | Secondary reducing agent |
| Resazurin | 0.001 | Redox indicator |
| Agar (semi-solid) | 0.5 | Oxygen gradient maintenance |
| Final pH (25 °C) | 7.1 ± 0.2 | |
Key Features
- Dual reducing system — sodium thioglycollate + L-cystine for robust anaerobic environment
- Resazurin redox indicator — visual, real-time monitoring of oxygen penetration
- Pharmacopoeial-grade — meets USP <71>, BP 2023, EP 2.6.1 requirements
- Semi-solid agar — maintains oxygen gradient for growth pattern interpretation
- No anaerobic jar required for routine tube culture
- Nutrient-rich — supports fastidious obligate anaerobes and microaerophiles
Applications
- Pharmaceutical sterility testing: USP <71>, BP 2023, EP 2.6.1 — parenteral products, biologics, medical devices
- Clinical microbiology: Cultivation of obligate anaerobes from blood, wound, and abscess specimens
- Research: Anaerobic physiology, environmental anaerobes, fermentation studies
Preparation
- Dissolve 28.5 g in 1 litre of purified water with gentle heating and agitation.
- Dispense into tubes or bottles as required.
- Autoclave at 121 °C for 15 minutes.
- Cool to room temperature, minimising oxygen exposure.
- Before use: heat in boiling water bath 10–15 min, cool rapidly without shaking.
- Discard any tube where >1/3 of depth is pink (over-oxidised).
CRITICAL: For pharmaceutical sterility testing — use only medium that is colourless or at most faintly pink in the upper layer (≤1/3 depth). Follow full pharmacopoeial preparation and validation requirements.
Growth Pattern Interpretation
| Observation | Organism Type | Action |
|---|---|---|
| Growth at tube bottom only | Obligate anaerobes | Confirm identity by subculture |
| Growth throughout tube | Facultative anaerobes | Confirm identity by subculture |
| Growth at surface only | Aerobes / microaerophiles | Confirm identity by subculture |
| Pink >1/3 from top | Oxygen penetration | Discard — re-prepare medium |
| No growth after 14 days (pharma) | Sterile | Pass — document per pharmacopoeia |
| Any growth after 14 days (pharma) | Not sterile | Fail — investigate and report |
Storage
| Dehydrated powder | 15–30 °C, tightly sealed, dry |
| Prepared medium | 2–8 °C, protected from light |
| Before use | Heat in boiling water bath 10–15 min; cool rapidly |
| Shelf life | As per labelled expiry date |
For Laboratory Use Only. Not for human, animal, or food consumption.
For laboratory use only. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
Product Identification
| Product Name | Thioglycollate Medium, Brewer Modified |
| Catalogue Number | AS-1364 |
| HS Code | 3821.00.00 |
| Medium Type | Enriched reducing semi-solid broth |
| Pharmacopoeial Equivalents | Fluid Thioglycollate Medium — USP <71>, BP 2023, EP 2.6.1 |
| Also known as | FTM, Fluid Thioglycollate Medium, THIO Broth, Brewer Thioglycollate |
Physical & Chemical Specifications
| pH (prepared, 25 °C) | 7.1 ± 0.2 |
| Moisture (loss on drying) | ≤ 5.0% |
| Agar concentration | 0.5 g/L (semi-solid) |
| Resazurin | 0.001 g/L — colourless (reduced) ↔ pink (oxidised) |
| Reducing system | Dual: sodium thioglycollate (0.5 g/L) + L-cystine (0.5 g/L) |
| Total solids | ~28.5 g/L |
Performance Specifications (Pharmacopoeial)
| Organism | ATCC No. | Expected Result | Ref. |
|---|---|---|---|
| Clostridium sporogenes | 11437 | Growth in lower zone (obligate anaerobe) | USP <71> / EP 2.6.1 |
| Bacteroides vulgatus | 8482 | Growth in lower zone | USP <71> |
| Staphylococcus aureus | 6538 | Growth throughout | USP <71> / EP 2.6.1 |
| Pseudomonas aeruginosa | 9027 | Growth at surface | USP <71> / EP 2.6.1 |
| Candida albicans | 10231 | Growth throughout | USP <71> |
| Bacillus subtilis | 6633 | Growth at surface | USP <71> / EP 2.6.1 |
Key Limitations
- Presumptive medium — confirmatory subculture required for organism identification
- Resazurin pink layer must not exceed 1/3 of tube depth before inoculation
- For pharmacopoeial sterility testing — must be prepared and validated per applicable pharmacopoeia
- Do not overheat — degrades reducing agents and nutrients
Version 1.0 — March 2026. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
GHS Classification: Mixture not classified as hazardous at use concentration (AU WHS Regulations 2011) | Note: Sodium thioglycollate component — H302 Harmful if swallowed (at high concentration) | ADG: Not regulated | For laboratory use only.
Section 1 — Identification
| Product Name | Thioglycollate Medium, Brewer Modified |
| Catalogue Number | AS-1364 |
| Intended Use | Enriched reducing medium for anaerobic cultivation and pharmaceutical sterility testing — laboratory use only |
| Supplier | AuSaMicS Pty Ltd | 31 Longview CT, Thomastown VIC 3074 |
| ABN | 56 676 640 467 |
| Emergency Phone | +61 412 520 598 | Poisons Information: 13 11 26 (24 hr) |
| support@ausamics.com.au |
Section 3 — Composition
| Component | CAS | % | Classification |
|---|---|---|---|
| Pancreatic Digest of Casein | 73049-73-7 | ~52.6% | Not classified |
| Yeast Extract | 8013-01-2 | ~17.5% | Not classified |
| Dextrose | 50-99-7 | ~8.8% | Not classified |
| Sodium Chloride | 7647-14-5 | ~17.5% | Not classified |
| Sodium Thioglycollate | 367-51-1 | ~1.75% | H302, H311, H331 (at high concentration) |
| L-Cystine | 56-89-3 | ~1.75% | Not classified |
| Resazurin | 62758-13-8 | <0.01% | Not classified at this level |
| Agar | 9002-18-0 | ~1.75% | Not classified |
Sections 4–8 — First Aid, Handling & PPE
| Topic | Guidance |
|---|---|
| Inhalation | Remove to fresh air. Seek medical advice if symptoms persist. |
| Skin Contact | Wash with soap and water ≥15 minutes. Note: sodium thioglycollate may cause sensitisation. |
| Eye Contact | Flush with water ≥15 minutes. Seek medical attention. |
| Ingestion | Do not induce vomiting. Rinse mouth. Seek medical advice. Inform physician of sodium thioglycollate content. |
| Inoculated spill | BSL-2 precautions. Disinfect with 1% sodium hypochlorite. Autoclave before disposal. |
| PPE | Nitrile gloves, safety glasses, lab coat. Biosafety cabinet for inoculated work. |
Section 13 — Disposal
Uninoculated: Chemical waste per local EPA regulations. Neutralise reducing agents before small-quantity drain disposal.
Inoculated medium: Autoclave at 121 °C for 30 minutes. Treat as BSL-2 biological waste.
Section 15 — Regulatory
| WHS Regulations 2011 | Mixture not classified as hazardous at use concentration |
| Sodium thioglycollate | Listed on AIIC — handle with appropriate care |
| Transport (ADG/IATA) | Not classified as dangerous goods |
Prepared per Australian WHS Regulations and GHS 7th Edition. Version 1.0 — March 2026. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
Product Information
| Product Name | Thioglycollate Medium, Brewer Modified |
| Catalogue Number | AS-1364 |
| Pharmacopoeial Ref. | Fluid Thioglycollate Medium — USP <71>, BP 2023, EP 2.6.1 |
| Lot / Batch Number | Contact us for batch-specific COA |
| Manufacturing Date | Available on request |
| Expiry Date | Available on request |
| Storage Conditions | 15–30 °C, tightly sealed, dry; prepared: 2–8 °C, protect from light |
Specification & Test Results
| Test | Specification | Typical Result | Status |
|---|---|---|---|
| Appearance (powder) | Cream to light beige powder | Complies | ✓ PASS |
| Colour (prepared, reduced) | Colourless to faintly pink (surface ≤1/3) | Complies | ✓ PASS |
| pH (prepared, 25 °C) | 7.1 ± 0.2 | Complies | ✓ PASS |
| Moisture (loss on drying) | ≤ 5.0% | Complies | ✓ PASS |
| Solubility | Completely soluble with gentle heating | Complies | ✓ PASS |
| Resazurin indicator function | Pink (oxidised); colourless (reduced) | Complies | ✓ PASS |
| C. sporogenes ATCC 11437 | Growth in lower zone | Complies | ✓ PASS |
| S. aureus ATCC 6538 | Growth throughout | Complies | ✓ PASS |
| P. aeruginosa ATCC 9027 | Growth at surface | Complies | ✓ PASS |
| C. albicans ATCC 10231 | Growth throughout | Complies | ✓ PASS |
| Oxidation limit | Pink layer ≤1/3 total depth | Complies | ✓ PASS |
Authorisation
| Released by | Hassan Salimi |
| Title | Founder and Technical Director |
| Organisation | AuSaMicS Pty Ltd |
Need a batch-specific COA?
Contact us with your order number and we will issue a full COA within 1 business day.
support@ausamics.com.au | +61 412 520 598
Contact us with your order number and we will issue a full COA within 1 business day.
support@ausamics.com.au | +61 412 520 598
This COA is valid for the batch identified above. Not transferable to other batches. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au