Tricine

Tricine

High-Purity Buffering Agent for Biochemical & Molecular Biology Applications

Product Identification

Chemical Information

Product Name: Tricine
Synonym: N-[Tris(hydroxymethyl)methyl]glycine
Catalogue Number: ASC-1057
CAS Number: 5704-04-1
Molecular Formula: C₆H₁₃NO₅
Molecular Weight: 179.17 g/mol
Form: Crystalline powder
Purity: ≥ 99 %

Product Overview

Tricine is a high-purity zwitterionic buffering agent widely used in biochemical and molecular biology research. It is particularly valued for its effective buffering range of pH 7.4–8.8, making it suitable for experiments requiring stable, near-neutral to mildly alkaline conditions.

Ausamics Tricine is supplied as a ≥ 99 % pure powder with no fillers, ensuring reproducible performance in sensitive applications such as protein electrophoresis, enzyme assays, and cell-based workflows. Its excellent water solubility allows for rapid preparation and precise pH adjustment.

Functional Role

Buffering Characteristics

Maintains stable pH in biological systems
Low metal-binding properties compared to some phosphate buffers
Minimal interference with enzymatic and protein-based assays

Applications

Protein & Molecular Biology

SDS-PAGE and Tricine-SDS-PAGE systems
Protein separation and stabilisation
Electrophoresis buffer preparation

Biochemical Research

Enzyme activity and kinetic assays
pH-sensitive biochemical reactions

Cell Culture & Laboratory Use

Buffer component in specialised culture systems
General laboratory buffer preparation

Key Features

Product Advantages

High purity (≥ 99 %) for reproducible results
Effective buffering range of pH 7.4–8.8
Freely soluble in water
Suitable for protein, enzyme, and molecular biology workflows
Consistent batch-to-batch quality

Physical Properties

Typical Characteristics

Appearance: White crystalline powder
Solubility: Soluble in water
Odour: Odourless

Storage & Stability

Storage Conditions

Store at 15–25 °C
Keep container tightly sealed
Protect from moisture

Stable under recommended storage conditions.

Intended Use & Safety

Regulatory Statement

For laboratory research use only.
Not intended for human, animal, food, diagnostic, or therapeutic use.

Refer to the Safety Data Sheet (SDS) prior to use.

Packaging & Availability

Pack Sizes

10 g
25 g
50 g
100 g
250 g

Bulk quantities available on request.

Ordering & Support

Supplier Information

Ausamics Pty Ltd
📧 support@ausamics.com
📍 Melbourne, Australia

Technical support and bulk pricing available.