Trimethoprim

Trimethoprim

Dihydrofolate Reductase Inhibitor for Microbiology & Resistance Research

Product Identification

Chemical Information

Product Name: Trimethoprim
Synonym: 2,4-Diamino-5-(3,4,5-trimethoxybenzyl)pyrimidine
Catalogue Number: AS-2024
CAS Number: 738-70-5
Molecular Formula: C₁₄H₁₈N₄O₃
Molecular Weight: 290.32 g/mol
Form: White to off-white crystalline powder
Purity: ≥ 98 % (research grade)

Product Overview

Trimethoprim is a bacteriostatic antimicrobial compound widely used in microbiology and molecular biology research. It acts as a selective inhibitor of bacterial dihydrofolate reductase (DHFR), thereby blocking folate synthesis and indirectly inhibiting nucleic acid and protein production.

In laboratory settings, Trimethoprim is commonly employed as a selective agent in culture media, as well as in antimicrobial susceptibility testing and mechanistic studies of folate metabolism and resistance pathways.

Ausamics Trimethoprim is supplied at high purity to ensure reliable and reproducible performance in research and analytical applications.

Mechanism of Action

Functional Activity

Inhibits bacterial dihydrofolate reductase (DHFR)
Prevents tetrahydrofolate synthesis
Disrupts DNA and RNA synthesis
Results in inhibition of bacterial cell growth

Solubility Characteristics

Solvent Compatibility

Slightly soluble in water
Soluble in ethanol
Soluble in DMSO

Stock solutions are typically prepared in organic solvents and diluted as required.

Applications

Microbiology & Antibiotic Research

Antimicrobial susceptibility testing
Selective agent in microbiological culture media
Resistance mechanism studies

Molecular Biology & Biochemistry

Folate metabolism and enzyme inhibition studies
Investigation of DHFR structure–function relationships

Research & Screening

Comparative antimicrobial screening
Evaluation of resistance development and gene expression

Key Features

Product Advantages

Selective DHFR inhibitor with well-characterised activity
High purity (≥ 98 %) for reproducible research outcomes
Compatible with selective media formulations
Suitable for resistance and metabolic pathway studies

Storage & Stability

Storage Conditions

Store at +4 °C
Keep container tightly sealed
Protect from light and moisture

Stable under recommended storage conditions.

Handling & Safety

Safety Information

May cause irritation to skin, eyes, or respiratory tract

Handling Precautions

Use appropriate personal protective equipment (PPE)
Handle in a well-ventilated area or fume hood

Refer to the Safety Data Sheet (SDS) for full handling and safety guidance.

Intended Use & Regulatory Statement

Usage Disclaimer

For laboratory research use only.
Not intended for human or veterinary use.
Not for therapeutic, diagnostic, or food applications.

The purchaser is responsible for compliance with applicable regulations.

Packaging & Availability

Pack Sizes

1 g
5 g
10 g
Bulk quantities available on request