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Tryptic Soy Broth (TSB) - Non-Selective Microbiological Culture Medium - AS-1371
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Documentation
Tryptic Soy Broth (TSB)
Non-Selective Microbiological Culture Medium
Catalog No: AS-1371 Alternate Names: CASO Broth, Trypticase Soy Broth Dehydrated Powder HS Code: 3821.00
General-Purpose Broth for Broad Microbial Cultivation
Tryptic Soy Broth (TSB) is a general-purpose, non-selective liquid culture medium used for the cultivation, enrichment, and maintenance of a wide range of microorganisms. Its balanced formulation, based on casein and soybean digests, provides amino acids, peptides, vitamins, carbohydrates, and salts required for reliable microbial growth.
TSB supports both fastidious and non-fastidious organisms and is widely used in food microbiology, environmental testing, quality control, research laboratories, inoculum preparation, and sterility-related workflows.
Why labs use it
- Non-selective medium supporting broad microbial growth
- Balanced nutrient composition for reliable performance
- Suitable for routine culture and enrichment
- Consistent and reproducible results
Typical applications
- Food and environmental microbiology
- General microbial cultivation and maintenance
- Quality control and sterility testing
- Preparation of inocula and enrichment cultures
Typical Composition (per liter)
| Component | Amount |
|---|---|
| Pancreatic digest of casein | 17.0 g |
| Papaic digest of soybean meal | 3.0 g |
| Sodium chloride | 5.0 g |
| Dipotassium phosphate | 2.5 g |
| Dextrose | 2.5 g |
| Final pH | 7.3 ± 0.2 at 25 °C |
Preparation (General)
- Suspend 30 g of dehydrated powder in 1 L purified water
- Heat gently with agitation until fully dissolved
- Dispense as required
- Sterilise by autoclaving at 121 °C for 15 minutes
- Cool to room temperature before use
Intended Use: For laboratory use only, including food microbiology, environmental testing, quality control, and research applications. Not for human or veterinary use. Not for consumption.
Technical Data Sheet (TDS)
| Product Name | Tryptic Soy Broth (TSB) |
|---|---|
| Catalog Number | AS-1371 |
| Alternate Names | CASO Broth, Trypticase Soy Broth, Soybean–Casein Digest Medium |
| Form | Dehydrated powder |
| Prepared Medium | Clear to slightly opalescent broth |
| HS Tariff Code | 3821.00 |
Technical Overview
TSB is a versatile, non-selective broth medium formulated for broad microbial cultivation. The combination of casein and soybean digests supplies essential growth nutrients, while sodium chloride and buffering salts help maintain suitable osmotic balance and pH stability during incubation.
Recommended Applications
- General cultivation of bacteria and other microorganisms
- Preparation and maintenance of inocula
- Enrichment procedures
- Quality control and sterility-related testing
Preparation Instructions
- Suspend 30 g in 1 L purified water
- Heat gently with agitation until dissolved
- Dispense into suitable containers
- Autoclave at 121 °C for 15 minutes
- Cool before inoculation or use
Storage
- Dehydrated powder: Store at 15–30 °C in a dry, sealed container
- Prepared broth: Store at 2–8 °C
- Protect from moisture and light
End users should verify suitability for their own organisms, validation methods, and application-specific requirements.
Safety Data Sheet (SDS) Summary
1. Identification
Product Name: Tryptic Soy Broth (TSB)
Catalog Number: AS-1371
Recommended Use: Laboratory microbiological culture medium
2. Hazard Identification
Not generally classified as hazardous based on typical GHS criteria for this product form. Avoid dust inhalation and follow standard laboratory handling precautions.
3. Composition / Information on Ingredients
Prepared culture medium in dehydrated powder form containing casein digest, soybean digest, sodium chloride, dipotassium phosphate, and dextrose.
4. First Aid Measures
- Inhalation: Move to fresh air if dust is inhaled.
- Skin Contact: Wash with soap and water.
- Eye Contact: Rinse cautiously with water for several minutes.
- Ingestion: Rinse mouth. Seek medical advice if unwell.
5. Fire-Fighting Measures
Use extinguishing media appropriate to surrounding materials. Minimise dust dispersion during fire response.
6. Accidental Release Measures
Collect spilled material carefully to minimise dust formation. Dispose according to applicable local regulations.
7. Handling and Storage
- Handle using good laboratory practice
- Avoid unnecessary dust generation
- Store tightly closed in a dry place protected from moisture and light
8. Exposure Controls / Personal Protection
Use appropriate laboratory PPE including gloves, eye protection, and protective clothing where required.
13. Disposal Considerations
Dispose of contents and container in accordance with local, regional, and national regulations.
This is a website SDS summary only. A full formal SDS should be supplied separately for compliance, procurement, and safe-use documentation.
Certificate of Analysis (COA) Summary
| Product Name | Tryptic Soy Broth (TSB) |
|---|---|
| Catalog Number | AS-1371 |
| Form | Dehydrated powder |
| Prepared Medium | Clear to slightly opalescent broth |
| Batch Number | To be stated on batch COA |
| Manufacture Date | To be stated on batch COA |
| Expiry Date | To be stated on batch COA |
Typical Release Criteria
| Test | Specification |
|---|---|
| Appearance | Light beige to tan dehydrated powder |
| Prepared Medium | Clear to slightly opalescent broth |
| pH (25 °C) | 7.3 ± 0.2 |
| Performance | Supports broad microbial cultivation for intended applications |
| Batch Status | Released after QC review |
COA Available
Batch-specific certificate available on request or with supply.
SDS Available
Formal safety documentation available for compliance and procurement.
TDS Available
Technical specification sheet available for validation and QC review.
Values shown here are summary placeholders for web presentation. Final released COAs should include lot-specific results, authorised review, and issue date.