Wort Agar – Microbiological Culture Medium
Wort Agar – Microbiological Culture Medium
AuSaMicS Life Science™ | www.ausamics.com.au
Wort Agar is a nutrient-rich microbiological medium formulated for the isolation, cultivation, and enumeration of yeasts and molds, particularly organisms associated with brewing, fermentation, and beverage microbiology. Malted wort contributes essential carbohydrates, vitamins, and minerals, while peptones provide nitrogen and amino acids to support robust fungal growth.
This medium is especially useful in fermentation research, beverage production quality control, and environmental monitoring programs where recovery of yeasts and molds is required. Its balanced nutrient profile makes it suitable for general fungal cultivation as well as growth of more demanding spoilage organisms encountered in brewery, winery, and food industry settings.
- Isolation and propagation of yeasts and molds from brewing, fermentation, and beverage samples
- General cultivation of fungi in laboratory settings
- Microbiological quality control in breweries, wineries, and food industries
- Monitoring spoilage organisms in production systems
- Yeast fermentation and biomass production studies
- Environmental monitoring in beverage production facilities
| Step | Procedure |
|---|---|
| 1 | Dissolve 40–50 g of medium in 1 L of purified water. |
| 2 | Heat with gentle agitation until fully dissolved. |
| 3 | Dispense into Petri dishes, slants, or tubes as required. |
| 4 | Sterilize by autoclaving at 121 °C for 15 minutes. |
| 5 | Cool to 45–50 °C before pouring plates or preparing slants. |
Safety Data Sheet
Wort Agar – Microbiological Culture Medium | Cat. AS-1402
| Component | Typical Content | Function |
|---|---|---|
| Malt Extract / Wort | 20–30 g/L | Carbohydrate, vitamin, and mineral source |
| Peptone / Proteose Peptone | 5 g/L | Nitrogen and amino acid source |
| Agar | 15 g/L | Solidifying agent |
| Purified Water | q.s. to 1 L | Solvent / medium base |
Certificate of Analysis
Wort Agar – Microbiological Culture Medium | Cat. AS-1402
| No. | Test / Criteria | Method | Specification / Limit | Result | Status |
|---|---|---|---|---|---|
| 1 | Appearance | Visual inspection | Homogeneous dehydrated powder | Conforms | PASS |
| 2 | Colour of Medium | Visual inspection | Conforms to standard | Conforms | PASS |
| 3 | pH (prepared medium) | Potentiometry | 5.4–5.6 ± 0.2 at 25 °C | 5.5 | PASS |
| 4 | Gel Strength / Solidification | Preparation test | Firm gel after sterilization | Conforms | PASS |
| 5 | Growth Promotion | Biological performance test | Supports growth of representative yeasts and molds | Conforms | PASS |
| 6 | Sterility Check | Incubation / QC | No contamination in uninoculated control | Conforms | PASS |