Yeast Extract Peptone Dextrose (YPD) Broth
Yeast Extract Peptone Dextrose (YPD) Broth
Nutrient-Rich Non-Selective Liquid Medium for Yeast Cultivation
Product Identification
Chemical & Physical Information
Product Name: Yeast Extract Peptone Dextrose Broth
Synonyms: YPD Broth; YEPD Broth
Catalogue Number: AS-1385
Medium Type: Non-selective liquid culture medium
Form: Dehydrated powder
Product Description
Yeast Extract Peptone Dextrose (YPD) Broth is a nutrient-rich, non-selective liquid culture medium widely used for the cultivation, propagation, and maintenance of yeasts, particularly Saccharomyces cerevisiae. Its balanced formulation provides readily assimilable nitrogen, vitamins, and carbohydrates, supporting rapid biomass generation and vigorous yeast growth.
YPD Broth is a standard medium in molecular biology, yeast genetics, fermentation research, and industrial biotechnology, where high cell density and reproducible growth are required. The non-selective nature of the medium allows cultivation of a broad range of yeast strains without selective pressure.
Ausamics YPD Broth is supplied as a high-quality dehydrated powder to ensure clarity, consistency, and batch-to-batch reproducibility.
Principle of the Medium
Functional Components
Yeast Extract: Provides B-complex vitamins, growth factors, and amino acids
Peptone: Supplies peptides and nitrogenous compounds
Dextrose (Glucose): Primary fermentable carbohydrate and energy source
Typical Characteristics
Medium Properties
Appearance (dehydrated): Light beige powder
Appearance (prepared): Clear to slightly opalescent light amber broth
pH (prepared medium): 6.5 ± 0.2 at 25 °C
Selectivity: Non-selective
Differential: No
Composition
Standard Formula (per litre)
Peptone: 20.0 g
Yeast Extract: 10.0 g
Dextrose (Glucose): 20.0 g
Typical Applications
Yeast Cultivation & Maintenance
Routine growth of Saccharomyces cerevisiae
Propagation and maintenance of laboratory yeast strains
Molecular Biology & Genetics
Yeast transformation and expression studies
Plasmid propagation and phenotype analysis
Fermentation & Industrial Biotechnology
Yeast growth for fermentation research
Bioprocess development and optimisation studies
Education & Research
Teaching laboratories and microbiology practicals
General yeast physiology and metabolism research
Preparation Instructions
Medium Preparation
Suspend 50 g of dehydrated medium in 1 L distilled or purified water
Heat gently if required until fully dissolved
Sterilise by autoclaving at 121 °C for 15 minutes
Cool to room temperature before use
Storage & Stability
Storage Conditions
Dehydrated medium: Store at 15–30 °C in a cool, dry place
Keep container tightly closed and protected from moisture
Prepared broth: Store at 2–8 °C
Use within the recommended period to ensure optimal performance
Intended Use
Regulatory Statement
For laboratory research, industrial biotechnology, and educational use only.
Not intended for medical, diagnostic, food, or veterinary use.
Quality & Supply Assurance
Manufacturing & Documentation
Manufactured under controlled conditions
Consistent batch-to-batch performance
Certificate of Analysis (COA) available upon request
Technical support provided by Ausamics Life Science
Customs & Trade Information
Classification
HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms