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APT Broth | All-Purpose Tween 80 Broth | Lactic Acid Bacteria
$39.00 AUD
Documentation
APT Broth (All Purpose Tween Broth)
Non-Selective Liquid Medium for Heterofermentative Lactobacilli, Thiamine Assay & High-Thiamine Organisms | Cat. No. AS-1121
Cat. No. AS-1121 pH 6.7 ± 0.2 46.2 g/L HiMedia M227 Equivalent 🇦🇺 Made in Melbourne ⚡ Same-Week Dispatch
Who uses this: 🥩 Food & Meat QC Labs 🏭 Dairy & Beverage QC 💊 Thiamine Assay Labs 🎓 University Food Micro Depts 🔬 NATA-Accredited Labs
📄 Full DocumentationCOA, TDS & SDS every batch
🇦🇺 Australian StockNo import delays
⚡ Same-Week DispatchEpping, Melbourne warehouse
🔬 Technical SupportDirect from our formulation team
In stock — Melbourne warehouse. Orders placed before 2pm AEST typically dispatch same day.
Overview
APT Broth (AS-1121) — All Purpose Tween Broth — is the liquid counterpart of AS-1120 APT Agar, formulated per the original Deibel/Evans/Niven specification. It is a non-selective, nutrient-rich liquid medium for the cultivation and maintenance of heterofermentative lactic acid bacteria and organisms with a high thiamine requirement, and is the standard inoculum-preparation medium for Weissella viridescens ATCC 12706 in the microbiological thiamine assay.
AS-1121 is manufactured in Melbourne to the verified original formula — identical to AS-1120 APT Agar minus the agar solidifying agent — including the full trace-mineral system (manganese chloride, magnesium sulfate, ferrous sulfate) and thiamine hydrochloride required for lactobacilli replication.
Mode of Action
Nutrient-Rich, Non-Selective Liquid Recovery for Fastidious Lactic Acid Bacteria
Casein Peptone and Yeast Extract supply amino acids, peptides, and B-complex vitamins — including the high thiamine levels lactobacilli need for growth. Dextrose is the fermentable carbohydrate energy source. Sodium Citrate and Dipotassium Phosphate buffer the medium and partially suppress competing Gram-negative flora. Manganese Chloride, Magnesium Sulfate, and Ferrous Sulfate supply the metallic ions lactobacilli require for enzymatic replication. Tween 80 (Polysorbate 80) supplies the fatty acids many lactobacilli cannot synthesise themselves. As a liquid medium, APT Broth is used both for direct enrichment/cultivation and as the inoculum-preparation step before plating onto APT Agar or assay procedures.
Casein Peptone and Yeast Extract supply amino acids, peptides, and B-complex vitamins — including the high thiamine levels lactobacilli need for growth. Dextrose is the fermentable carbohydrate energy source. Sodium Citrate and Dipotassium Phosphate buffer the medium and partially suppress competing Gram-negative flora. Manganese Chloride, Magnesium Sulfate, and Ferrous Sulfate supply the metallic ions lactobacilli require for enzymatic replication. Tween 80 (Polysorbate 80) supplies the fatty acids many lactobacilli cannot synthesise themselves. As a liquid medium, APT Broth is used both for direct enrichment/cultivation and as the inoculum-preparation step before plating onto APT Agar or assay procedures.
Applications
💊
Thiamine Assay
Inoculum prep — Weissella viridescens ATCC 12706
🥫
Canned & Cured Meats
Greening/spoilage lactobacilli enrichment
🥛
Dairy QC
Lactic acid bacteria cultivation
🍺
Beverage Spoilage
Fruit juice & fermented beverage QC
🧫
Stock Maintenance
Liquid subculture prior to plating on AS-1120
🎓
Research & Teaching
Lactic acid bacteria taxonomy & physiology
APT Broth vs APT Agar — Workflow Position
| Step | Medium | Purpose |
|---|---|---|
| 1. Enrichment / inoculum prep | APT Broth (AS-1121) | Liquid cultivation, thiamine assay inoculum, sample enrichment |
| 2. Isolation / enumeration | APT Agar (AS-1120) | Colony isolation, plate count, morphology assessment |
| 3. Confirmation (optional) | Biochemical / molecular methods | Species-level identification |
FAQs
❓ What is APT Broth used for in the thiamine assay?
Weissella viridescens ATCC 12706 (formerly Lactobacillus viridescens) requires thiamine for growth, making it the standard test organism for microbiological thiamine assays. APT Broth is used to prepare the working inoculum from stock cultures maintained on APT Agar, following the method described by Deibel, Evans & Niven.
❓ How is APT Broth different from APT Agar?
APT Broth is compositionally identical to APT Agar except that the agar solidifying agent is omitted, giving 46.2 g/L versus 61.2 g/L for the agar. Preparation only requires warming to dissolve (no boiling step is needed as there is no agar to melt) before autoclaving.
❓ Is APT Broth selective for lactobacilli?
No — like APT Agar, APT Broth is non-selective. Coliforms and other common bacteria will grow readily. Confirmatory biochemical, serological, or molecular testing on isolates is recommended for definitive identification.
❓ Is AS-1121 equivalent to HiMedia M227 or Hardy CRITERION APT Broth?
Yes — AS-1121 follows the same verified original APT Broth formula used by HiMedia (M227) and Hardy Diagnostics (CRITERION). AS-1121 is manufactured in Melbourne with same-week dispatch and full Australian batch documentation — no import delays.
Cross-Reference / Equivalent Products
| Supplier | Product Name | Cat. No. | AuSaMicS Advantage |
|---|---|---|---|
| HiMedia | APT Broth | M227 | Melbourne stock; Australian regulatory compliance |
| Hardy Diagnostics | CRITERION™ APT Broth | — | Australian manufactured — no import lead times |
| Liofilchem | APT Broth | — | Full GHS documentation included; same-week dispatch |
Related Products
Product Identification
| Product Name | APT Broth (All Purpose Tween Broth) |
| Common Names | APT Broth; All Purpose Tween Broth |
| Catalogue Number | AS-1121 |
| Solid Counterpart | APT Agar (AS-1120) |
| Reference Standard | Deibel, Evans & Niven (1957); Evans & Niven (1951) original formulation |
| Reconstitution | 46.2 g/L |
| Final pH at 25°C | 6.7 ± 0.2 |
| Sterilisation | Autoclave 121°C for 15 minutes |
| Dehydrated Appearance | Homogeneous, free-flowing, light beige powder |
| Prepared Appearance | Clear to slightly opalescent, light amber broth |
| Shelf Life | 2 years from manufacture in original sealed packaging |
| Equivalents | HiMedia M227 | Hardy Diagnostics CRITERION APT Broth |
Formula — Per Litre (Verified Original Specification)
✓ Verified formula — identical to AS-1120 APT Agar with agar omitted, cross-checked against Hardy Diagnostics CRITERION APT Agar/Broth technical data and HiMedia M226/M227 published specifications.
| Ingredient | Amount | Function |
|---|---|---|
| Casein Peptone | 12.5 g | Amino acids, peptides, nitrogen source |
| Dextrose | 10.0 g | Fermentable carbohydrate energy source |
| Yeast Extract | 7.5 g | B-complex vitamins, high thiamine content |
| Sodium Chloride | 5.0 g | Osmotic balance |
| Sodium Citrate | 5.0 g | Buffering; partial suppression of Gram-negative flora |
| Dipotassium Phosphate | 5.0 g | Buffering agent |
| Magnesium Sulfate | 0.8 g | Enzyme cofactor |
| Tween 80 (Polysorbate 80) | 0.2 g | Fatty acid source for lactobacilli |
| Manganese Chloride | 0.14 g | Essential trace ion for lactobacilli replication |
| Ferrous Sulfate | 0.04 g | Trace iron; enzymatic cofactor |
| Thiamine Hydrochloride | 0.001 g | Essential vitamin — high-thiamine-requiring organisms |
| Total | 46.2 g/L | Final pH 6.7 ± 0.2 at 25°C |
Preparation Protocol
| 1 | Suspend 46.2 g of dehydrated powder in 1 litre of distilled or deionised water. Mix thoroughly. |
| 2 | Warm slightly with agitation to dissolve completely. Do not boil — unlike the agar, no melting step is required. |
| 3 | Verify pH is 6.7 ± 0.2 at 25°C before sterilisation. Adjust if necessary. |
| 4 | Dispense into tubes, bottles, or flasks as required. |
| 5 | Autoclave at 121°C for 15 minutes. Avoid overheating. |
| 6 | Cool to room temperature before inoculation. Store prepared broth at 2–8°C. |
⚠️ Because APT Broth is non-selective, coliforms and other common contaminants will grow readily. Confirmatory biochemical or molecular testing is required for definitive identification of lactic acid bacteria isolates.
Quality Control — Performance Organisms
| Organism (ATCC) | Inoculum | Incubation | Expected Result |
|---|---|---|---|
| Weissella viridescens (12706) | Stab / loop transfer | 35–37°C / 24–48h, aerobic | Good growth — thiamine assay inoculum |
| Lactobacillus fermentum (9338) | ≤100 CFU | 35°C / 24–48h, aerobic | Good growth, turbidity |
Storage
| Dehydrated Powder | 2–30°C, tightly sealed, protected from moisture and light |
| Prepared Broth | 2–8°C; use within 7 days |
Literature References
- Evans JB, Niven CF Jr. Nutrition of the heterofermentative lactobacilli that cause greening of cured meat products. J Bacteriol. 1951;62:599–603.
- Deibel RH, Evans JB, Niven CF Jr. Microbiological assay for thiamine using Lactobacillus viridescens. J Bacteriol. 1957;74:818–821.
- APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods. Washington, D.C.: APHA.
- ISO 11133:2014. Microbiology of food, animal feed and water — culture media performance testing.
📄 Full 16-section GHS SDS available (Australian WHS Regulations 2023 / GHS 7th Edition) — support@ausamics.com
Section 1 — Identification
| Product Name | APT Broth (All Purpose Tween Broth) |
| Catalogue No. | AS-1121 |
| Supplier | AuSaMicS Pty Ltd | ABN 56 676 640 467 |
| Address | 36/25 Trafalgar Road, Epping, VIC 3076, Australia |
| Emergency | Poisons Information Centre: 13 11 26 (24 hr) |
| Phone | +61 412 520 598 |
| support@ausamics.com |
Section 2 — Hazard Identification
✓
Not classified as hazardous — GHS / Safe Work Australia No GHS hazard pictograms, signal word, or hazard statements are assigned under Safe Work Australia GHS criteria for the dehydrated powder. This product does not meet the threshold criteria for any hazard category in the Hazardous Chemical Information System (HCIS).
| Signal Word | None required |
| Hazard Statements | None assigned |
| Precautionary Statements | P260 — Do not breathe dust | P264 — Wash hands thoroughly after handling | P272 — Contaminated clothing not to leave the workplace |
Laboratory Biosafety Notice: APT Broth is a microbiological growth medium. Once inoculated, it supports growth of bacteria including potential contaminant pathogens. Handle inoculated material under appropriate biosafety containment per AS/NZS 2243.3 and your institution's biosafety manual.
Section 3 — Composition / Information on Ingredients
| Component | Proportion (%) | HCIS Classification |
|---|---|---|
| Casein Peptone | ~27.1 | Not classified |
| Dextrose | ~21.6 | Not classified |
| Yeast Extract | ~16.2 | Not classified |
| Sodium Chloride | ~10.8 | Not classified |
| Sodium Citrate | ~10.8 | Not classified |
| Dipotassium Phosphate | ~10.8 | Mild irritant at high concentration |
| Magnesium Sulfate, Manganese Chloride, Ferrous Sulfate, Tween 80, Thiamine HCl | <3 combined | Not classified at use level |
First Aid & PPE Summary
| Eye Contact | Flush immediately with copious water 15 min. Seek medical attention if irritation persists. |
| Skin Contact | Wash affected area with soap and water. Seek medical attention if irritation develops. |
| Inhalation | Remove to fresh air. Seek medical attention if symptoms develop. |
| Ingestion | Do not induce vomiting. Rinse mouth. Seek medical advice. |
| PPE | Safety glasses, gloves, lab coat. |
| Biosafety Disposal | Autoclave all inoculated broth (121°C / 15 min minimum) before disposal per institutional procedures. |
| Transport | Not classified as Dangerous Goods — ADG, IATA, IMDG |
Quality Specifications
| Parameter | Specification | Method |
|---|---|---|
| Appearance (powder) | Homogeneous, free-flowing, light beige powder | Visual |
| Appearance (prepared) | Clear to slightly opalescent, light amber broth | Visual after preparation |
| pH (prepared, 25°C) | 6.7 ± 0.2 | pH meter (calibrated) |
| Moisture (powder) | ≤5.0% | Loss on drying |
| Growth Promotion | W. viridescens ATCC 12706 / L. fermentum ATCC 9338 — good growth, 24–48h | QC organism test |
| Sterility (uninoculated) | No growth at 35°C / 48h | Sterility check |
| Batch COA | Lot-specific, included with every order | — |
Pack Sizes
| 500 g | Makes ~10.8 litres prepared medium |
| 2 kg ⭐ | Makes ~43.3 litres prepared medium — most popular |
| Custom / Bulk | Contact support@ausamics.com for project-specific or bulk quantities |
Manufacturing & Documentation
Manufactured by AuSaMicS Pty Ltd
36/25 Trafalgar Road, Epping VIC 3076, Australia | ABN 56 676 640 467
✓ Formulated to the original verified APT Broth specification
✓ Full trace-mineral system (Mn, Mg, Fe) and thiamine HCl included in full
✓ COA, TDS, SDS included with every order
✓ Australian stock — same-week dispatch, no import delays
✓ Direct founder microbiologist technical support
36/25 Trafalgar Road, Epping VIC 3076, Australia | ABN 56 676 640 467
✓ Formulated to the original verified APT Broth specification
✓ Full trace-mineral system (Mn, Mg, Fe) and thiamine HCl included in full
✓ COA, TDS, SDS included with every order
✓ Australian stock — same-week dispatch, no import delays
✓ Direct founder microbiologist technical support
⚠️ For laboratory, food QC, and research use only. Not for human consumption, therapeutic, or veterinary use.