Salmon-Gal (S-Gal) | ASE-910 | 6-Chloro-3-indolyl β-D-galactopyranoside
Salmon-Gal (Red-Gal)
6-Chloro-3-indolyl β-D-galactopyranoside
Revolutionary chromogenic substrate producing distinctive salmon-red precipitate instead of traditional blue. Ideal for multiplex assays, immunohistochemistry, and applications requiring visual contrast differentiation.
🏆 The X-Gal Alternative
🔬 Enzymatic Mechanism & Signal Development
Salmon-Gal undergoes β-galactosidase-catalyzed hydrolysis, releasing 6-chloro-3-indoxyl which rapidly oxidizes and dimerizes to form an intense, insoluble salmon-red indigoid dye:
↓ (Enzymatic hydrolysis)
6-Chloro-3-indoxyl + Galactose
↓ (Spontaneous oxidation)
Salmon-Red Indigoid Precipitate
⚖️ β-Galactosidase Substrate Comparison
🧬 Specialized Research Applications
🔬 Immunohistochemistry
Enhanced visualization in tissue sections where red contrast improves detection against natural tissue colors.
🎯 Multiplex Detection
Simultaneous detection of multiple gene expressions using different colored substrates in single assays.
Additional Research Uses:
- Dual Reporter Systems: Red/blue enzyme discrimination
- Colony Screening: Alternative when blue interferes
- Plant Transformation: GUS reporter with contrast
- Food Microbiology: Visual contrast on yellow media
- Educational Labs: Demonstrating enzyme specificity
- Photography/Documentation: Better imaging results
💡 Protocol Optimization Guidelines
20-40 μg/mL (equivalent to X-Gal)
DMSO or DMF stock solution
37°C, 2-24h for color development
-20°C, desiccated, light protection
📋 Technical Specifications
| Chemical Name | 6-Chloro-3-indolyl β-D-galactopyranoside |
| CAS Number | 138182-21-5 |
| Formula | C₁₄H₁₆ClNO₆ |
| Molecular Weight | 329.73 g/mol |
| Purity | ≥98% (HPLC) |
| Appearance | White to off-white powder |
| Storage | -20°C, protect from light & moisture |
| HS Code | 2934.99.90 |
📝 Alternative Names
✅ Quality Standards
- ✓ HPLC Purity: ≥98% verified
- ✓ Identity: NMR confirmed
- ✓ Functionality: Enzymatic activity tested
- ✓ Stability: Storage conditions validated
- ✓ Documentation: Complete analytical package
🧬 Complete Your β-Galactosidase Toolkit
Need Application Guidance?
Our biochemists can help adapt your protocols for red signal detection
For laboratory, research, and industrial use only. Not for medicinal or food use.
AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
Same-day dispatch • Australian stock • Full documentation included