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Urea Agar Base (Christensen) | AS-1373 | Urease Detection | AuSaMicS
$35.00 AUD
AuSaMicS Life Science • Differential Media
Urea Agar Base
Christensen's Urease Detection Medium
Gold standard differential medium for urease activity detection. Critical for identifying Proteus, Klebsiella, Helicobacter, and other urease-positive organisms with distinctive phenol red color change.
AS-1373 🔴 Phenol Red ✓ Australian Stock 🧪 Biochemical
Same-Day Dispatch: Australian Stock Clinical Grade: Diagnostic applications Rapid Results: 1-48 hour detection
Urease Specialist
Color Change Expert - Phenol red system
Clinical Grade - Diagnostic applications
Complete Package - COA + Protocols
Fast Supply - Ships within 24h
Expert Support - Method optimization
pH Indicator System
Orange → Pink color change
🧪 Complete Formulation & Urease Mechanism
📋 Ingredient Composition (per Liter)
⚗️ Urease Hydrolysis Mechanism
Urease enzyme (if present)
↓
CO(NH₂)₂ + 2H₂O → 2NH₃ + CO₂
↓
NH₃ + H₂O → NH₄⁺ + OH⁻
↓
pH increases → Phenol Red: Yellow → Pink
↓
CO(NH₂)₂ + 2H₂O → 2NH₃ + CO₂
↓
NH₃ + H₂O → NH₄⁺ + OH⁻
↓
pH increases → Phenol Red: Yellow → Pink
NEGATIVE
Orange-Yellow (pH 6.8)
Orange-Yellow (pH 6.8)
POSITIVE
Pink-Red (pH >8.1)
Pink-Red (pH >8.1)
📊 Complete Urease Testing Media Portfolio
Strategic Method Selection for Urease Testing
✅ Urea Agar Advantages
- ✓ Highest Sensitivity: Detects weak urease activity
- ✓ Gold Standard: Most reliable method
- ✓ Stable Results: Clear endpoint determination
- ✓ Cost Effective: Simple formulation
- ✓ Long Shelf Life: Stable prepared medium
- ✓ Universal Application: All urease-positive organisms
⚠️ Method Considerations
- ▲ Time Variable: 1 hour to 7 days
- ▲ Preparation Required: vs. ready-to-use strips
- ▲ Heat Sensitivity: Urea degradation possible
- ▲ Storage Requirements: Light protection needed
- ▲ Single Test: vs. multiplex panels
🎯 Application-Specific Selection
- ◆ Clinical ID: Urea Agar (comprehensive testing)
- ◆ Rapid Proteus: Stuart's Broth (15 minutes)
- ◆ H. pylori: CLO Test (biopsy specimens)
- ◆ Point-of-Care: Dipstick methods
- ◆ Comprehensive ID: API panels
📊 Organism-Specific Urease Activity
🔬 Urease Testing Protocol & Quality Control
Inoculation:
Light streak on slant surface
Avoid heavy inoculation
Light streak on slant surface
Avoid heavy inoculation
Positive Control:
Proteus mirabilis ATCC 25933
Pink-red in 1-6 hours
Proteus mirabilis ATCC 25933
Pink-red in 1-6 hours
Negative Control:
E. coli ATCC 25922
Remains orange-yellow
E. coli ATCC 25922
Remains orange-yellow
Reading Schedule:
Check at 1h, 4h, 24h, 48h
Report time to positive
Check at 1h, 4h, 24h, 48h
Report time to positive
Critical Factors: Avoid heavy inoculation which can cause false positives due to protein breakdown. Incubate with loose caps to allow CO₂ escape.
📋 Technical Specifications
| Catalogue Number | AS-1373 |
| Dehydrated Weight | 44.0 g/L |
| Final pH | 6.8 ± 0.2 (at 25°C) |
| Urea Concentration | 20 g/L |
| Indicator | Phenol Red (12 mg/L) |
| Appearance (Powder) | Light pink to beige |
| Prepared Medium | Orange-yellow, clear slants |
| Sterilization | 121°C, 15 minutes max |
| Shelf Life | 3 years |
Key Urease-Positive Organisms
- ✓ Proteus mirabilis - UTI pathogen
- ✓ Proteus vulgaris - Wound infections
- ✓ Klebsiella pneumoniae - HAI pathogen
- ✓ Helicobacter pylori - Gastric ulcers
- ✓ Cryptococcus neoformans - Fungal
🏥 Clinical Applications
- ✓ UTI Diagnosis: Proteus identification
- ✓ Enterobacteriaceae ID: Species differentiation
- ✓ H. pylori Testing: Gastric biopsy specimens
- ✓ Fungal Identification: Yeast characterization
- ✓ Biochemical Profiling: IMViC battery
⚠️ Critical Success Factors
- Temperature Control: Cool before pouring (45-50°C)
- Reading Schedule: Check at multiple time points
- Light Inoculation: Avoid protein breakdown
- Light Protection: Store in darkness
- Loose Caps: Allow CO₂ escape
✅ Quality Standards
- ✓ Performance Testing: Reference strains
- ✓ pH Verification: 6.8 ± 0.2 confirmed
- ✓ Urea Potency: Substrate activity verified
- ✓ Color Development: Indicator function
- ✓ Stability Testing: Shelf life validation
📄 Documentation
🧪 Complete Biochemical Testing System
Differential & Biochemical Media
Reagents & Rapid Test Kits
Supporting Culture Media
Need Biochemical Testing Support?
Our clinical microbiologists can help optimize your urease testing and identification protocols
For clinical diagnostics and laboratory use only. Not for food, feed, household, cosmetic, therapeutic, or personal use.
AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
Same-day dispatch • Australian stock • Clinical compliance documentation included