HEPES Buffer

HEPES Buffer

$25.00
Weight

HEPES Buffer

High-Purity Zwitterionic Buffer for Molecular Biology & Cell Culture

Catalog Number: ASC-1058

Overview

HEPES Buffer (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) is a high-purity zwitterionic buffering agent widely used in molecular biology, cell culture, and biochemical research. It is valued for its excellent pH stability in the physiological range and its minimal interaction with biological systems.

HEPES effectively resists pH changes caused by CO₂ exchange and temperature variation, making it particularly suitable for applications where precise and stable pH control is required outside CO₂-controlled incubators.

Ausamics HEPES Buffer is supplied as a high-quality crystalline powder, manufactured for consistent purity, solubility, and reproducible performance in laboratory workflows.

Key Features & Benefits

  • Stable buffering capacity in the pH range 6.8–8.2

  • Zwitterionic structure with minimal biological interference

  • Highly soluble in water

  • Suitable for sensitive biological and biochemical systems

  • Reliable batch-to-batch consistency

Chemical & Physical Information

  • Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

  • CAS Number: 7365-45-9

  • Molecular Formula: C₈H₁₈N₂O₄S

  • Molecular Weight: 238.31 g/mol

  • Appearance: White crystalline powder

  • Solubility: Freely soluble in water

Typical Applications

Cell Culture

  • pH stabilisation for mammalian and microbial cell culture media

  • Use in systems sensitive to CO₂-related pH drift

Molecular Biology & Biochemistry

  • Buffering agent for enzyme assays and protein studies

  • pH control during nucleic acid and protein purification

Microbiology & Research Laboratories

  • Support buffer in experimental workflows

  • Educational and teaching laboratory applications

Principle of the Buffer

HEPES is a synthetic zwitterionic buffer designed to maintain a stable pH in biological systems. Its buffering capacity is largely independent of CO₂ concentration and temperature fluctuations, providing consistent conditions for cells, enzymes, and biomolecules.

Preparation & Use (Typical)

  • Dissolve HEPES powder in deionised water to prepare a stock solution (e.g. 1.0 M)

  • Adjust pH to the required value using sodium hydroxide or hydrochloric acid

  • Sterilise by filtration when required for cell culture applications

Storage & Stability

Store in a cool, dry place, protected from moisture and direct sunlight.
Keep container tightly sealed.

Stable under recommended storage conditions.

Intended Use

For laboratory research and educational use only.

Not for human, animal, diagnostic, or therapeutic use.

Quality & Documentation

  • Certificate of Analysis (COA) available

  • Safety Data Sheet (SDS) available upon request

  • Supplied under controlled quality systems

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