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Meat Extract Powder — High-Nutrient Base for Culture Media - AS-1005
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Meat Extract Powder
Bacteriological Grade | Animal-Derived Nutrient Base for Microbiological Culture Media | Cat. No. AS-1005
Cat. No. AS-1005 CAS: 68990-09-0 pH 6.0–7.5 (5% aq) Total N 11.5–12.5% ✓ Merck 103979 Equivalent ✓ COA / TDS / SDS Available 🇦🇺 Made in Melbourne ⚡ Same-Week Dispatch
Cat. No.
AS-1005
pH (5% aq)
6.0 – 7.5
Total Nitrogen
11.5 – 12.5%
Loss on Drying
≤ 6.0%
Typical Usage
3–10 g/L
📄 Full DocumentationCOA, TDS & SDS every batch
🇦🇺 Australian StockNo import delays
⚡ Same-Week DispatchMelbourne warehouse
🔬 Technical SupportDirect from our team
Overview
Meat Extract Powder (AS-1005) is a dehydrated, water-soluble concentrate prepared from beef muscle tissue. It is produced by controlled extraction, concentration, and drying of beef infusion to yield a standardised powder rich in free amino acids, short peptides, nucleotides, B-vitamins, creatine, and organic growth factors.
AS-1005 is the direct equivalent of Merck/Sigma-Aldrich Cat. 103979 (Meat Extract Granulated for Microbiology) and Oxoid LP0029, meeting the same verified specifications: Total nitrogen 11.5–12.5% (Kjeldahl), Amino nitrogen 3.5–4.5%, pH 6.0–7.5 (5% aqueous), Loss on drying ≤6.0%.
Functional Components
Free Amino Acids (dominant component): Glutamic acid, glycine, alanine, aspartic acid, leucine, and other amino acids in free form for direct microbial assimilation without further hydrolysis.
Short Peptides: Di- and tri-peptides from partial protein hydrolysis — readily available nitrogen source for rapid growth.
Nucleotides & Derivatives: Inosine, hypoxanthine, AMP metabolites — support DNA/RNA synthesis in fastidious organisms that cannot synthesise purines de novo.
B-Complex Vitamins: Thiamine (B1), riboflavin (B2), niacin (B3), pyridoxine (B6), cobalamin (B12) — essential metabolic co-factors.
Creatine & Creatinine: Characteristic of animal-derived extract — unique growth factors absent in plant-based alternatives such as yeast extract or soy peptone.
Trace Minerals: Fe, Zn, Mn, K, Mg naturally present from muscle tissue.
Short Peptides: Di- and tri-peptides from partial protein hydrolysis — readily available nitrogen source for rapid growth.
Nucleotides & Derivatives: Inosine, hypoxanthine, AMP metabolites — support DNA/RNA synthesis in fastidious organisms that cannot synthesise purines de novo.
B-Complex Vitamins: Thiamine (B1), riboflavin (B2), niacin (B3), pyridoxine (B6), cobalamin (B12) — essential metabolic co-factors.
Creatine & Creatinine: Characteristic of animal-derived extract — unique growth factors absent in plant-based alternatives such as yeast extract or soy peptone.
Trace Minerals: Fe, Zn, Mn, K, Mg naturally present from muscle tissue.
Applications
🧪
General Culture Media
Nutrient Agar, Nutrient Broth — 3 g/L standard dose
🔬
Fastidious Organisms
Streptococcus, Haemophilus, Bordetella requiring complex nutrients
💊
MRS Medium
Lactobacillus selective medium — 8–10 g/L
🧬
Anaerobic Cultivation
Strict and facultative anaerobes requiring growth factors
🍕
Food Microbiology
Salmonella, Listeria, coliform detection media
📚
Pharma QC & Research
Sterility testing, bioburden, teaching labs
Typical Usage Rates in Culture Media
| Medium | Meat Extract Dose | Notes |
|---|---|---|
| Nutrient Agar / Broth | 3.0 g/L | Standard WHO/ISO general-purpose medium |
| MRS Agar (De Man, Rogosa & Sharpe) | 8.0–10.0 g/L | Lactobacillus cultivation — ISO 15214 |
| Brain Heart Infusion (BHI) | 5.0 g/L | Fastidious organism enrichment |
| Cooked Meat Medium | Meat particles + 3g/L broth | Anaerobic cultivation |
| General enriched media | 5.0–10.0 g/L | Adjust per specific formulation requirements |
FAQs
❓ What is the difference between Meat Extract and Beef Extract?
They are the same product described by different names. "Meat Extract" and "Beef Extract" both refer to the dehydrated concentrate of bovine (cattle) muscle infusion, CAS 68990-09-0. Commercial names include Lab-Lemco (Oxoid), Beef Extract (BD Difco), and Meat Extract (Merck/Sigma). AS-1005 is equivalent to all of these.
❓ Is AS-1005 equivalent to Merck/Sigma 103979?
Yes — AS-1005 meets the same specification as Merck/Sigma-Aldrich Cat. 103979: pH 6.0–7.5 (5% aqueous), Total nitrogen 11.5–12.5% (Kjeldahl), Amino nitrogen 3.5–4.5%, Loss on drying ≤6.0% at 105°C, Sulfated ash ≤18.0%. Full COA with lot-specific results is provided with every order.
❓ Can Meat Extract be replaced by Yeast Extract or Soy Peptone?
Partially — but not fully. Yeast Extract and Soy Peptone cover most amino acid and B-vitamin requirements. However, Meat Extract contains creatine, creatinine, specific nucleotide fractions, and growth factors unique to animal muscle tissue that are absent in plant-based alternatives. For media requiring strict compliance with a named standard (e.g. Nutrient Agar ISO 6222, MRS ISO 15214), Meat Extract must be used as specified. For non-standardised media, validated functional equivalents may be acceptable.
❓ What is the amino nitrogen specification and why does it matter?
Amino nitrogen (3.5–4.5% as N) measures the free amino acid content of Meat Extract. This is the fraction immediately available for microbial uptake without further enzymatic processing. High amino nitrogen indicates a product rich in pre-formed, directly assimilable nutrients — critical for fastidious organisms and rapid growth media. The Kjeldahl total nitrogen (11.5–12.5%) includes all nitrogen forms including peptide-bound nitrogen.
Cross-Reference / Equivalents
| Manufacturer | Product Name | Cat. No. |
|---|---|---|
| Merck / Sigma-Aldrich | Meat Extract Granulated for Microbiology | 103979 |
| Thermo Scientific / Oxoid | Lab-Lemco Powder (Beef Extract) | LP0029 |
| BD Difco | Beef Extract | 212600 |
| HiMedia | Meat Extract Powder | RM002 |
Related Products
Product Specifications
| Product Name | Meat Extract Powder (Bacteriological Grade) |
| Catalogue Number | AS-1005 |
| CAS Number | 68990-09-0 |
| EINECS | 273-578-7 |
| Synonyms | Beef Extract Powder; Lab-Lemco; Meat Extract Dry; Beef Extract Desiccated |
| Equivalents | Merck/Sigma 103979 | Oxoid LP0029 | BD Difco 212600 | HiMedia RM002 |
| Grade | Microbiological / Bacteriological Grade |
| Form | Brown to dark brown dehydrated powder |
| Solubility | Readily soluble in water; insoluble in ethanol and organic solvents |
| pH (5% aqueous, 25°C) | 6.0 – 7.5 |
| Total Nitrogen (Kjeldahl) | 11.5 – 12.5% (w/w) |
| Amino Nitrogen (as N) | 3.5 – 4.5% (w/w) |
| Loss on drying (105°C) | ≤ 6.0% |
| Sulfated ash (800°C) | ≤ 18.0% |
| Nitrite (NO₂) | Passes test |
| Typical usage rate | 3–10 g/L depending on medium formulation |
| Storage | 10–30°C, dry, tightly closed, protected from light and moisture |
| Shelf life | 3 years from manufacture date in original sealed packaging |
| HS / AHECC Code | 2302.10.00 |
Preparation Guidance
1
Weigh the required amount of Meat Extract Powder per your medium formulation (typically 3–10 g/L).
2
Add to purified or distilled water with stirring. Dissolves readily at room temperature or with gentle heating.
3
Add remaining medium components per your formulation. Check pH — adjust if required before autoclaving.
4
Sterilise by autoclaving at 121°C for 15 minutes. Do not over-process — excess heat degrades growth factors.
Literature & References
| # | Reference |
|---|---|
| 1 | Merck KGaA / Sigma-Aldrich. Meat Extract Granulated for Microbiology, Cat. 103979. Product specification. [Reference standard for AS-1005] |
| 2 | ISO 11133:2014. Microbiology of food and water — Performance testing of culture media. Geneva: ISO. |
| 3 | De Man JC, Rogosa M, Sharpe ME. A medium for the cultivation of Lactobacilli. J Appl Bacteriol. 1960;23:130–135. |
| 4 | ISO 7218:2007. Microbiology of food — General requirements for microbiological examinations. Geneva: ISO. |
📄 Full 16-section GHS SDS available (Australian WHS Regulations 2023 / GHS 7th Edition) — support@ausamics.com.au
Section 1 — Identification
| Product Name | Meat Extract Powder (Bacteriological Grade) |
| Catalogue No. | AS-1005 |
| CAS Number | 68990-09-0 | EINECS: 273-578-7 |
| Intended Use | Ingredient in microbiological culture media. Laboratory and research use only. Not for food, therapeutic or household use. |
| Supplier | AuSaMicS Pty Ltd | ABN 56 676 640 467 |
| Address | 31 Longview CT, Thomastown VIC 3074, Australia |
| Emergency | Poisons Information Centre: 13 11 26 (24 hr) |
| Phone | +61 412 520 598 | support@ausamics.com.au |
Section 2 — Hazard Identification (GHS 7th Ed / WHS 2023)
| GHS Classification | NOT classified as a hazardous substance under Australian WHS Regulations 2023. |
| Signal Word | None required |
| Hazard Statements | None required |
| Other Hazards | Combustible organic powder. Dust may cause mild mechanical irritation. Biological origin — handle with standard laboratory PPE and hygiene practices. |
| Poison Schedule (AU) | Not a scheduled poison |
Sections 4–8 Summary
| Inhalation (S4) | Remove to fresh air. If irritation persists, seek medical advice. |
| Skin contact (S4) | Wash with soap and water for 15 minutes. |
| Eye contact (S4) | Rinse with water for at least 10 minutes. Seek advice if irritation persists. |
| Ingestion (S4) | Rinse mouth. Not acutely toxic. Seek medical advice if large quantity ingested. |
| Fire (S5) | Combustible powder. Use CO₂, dry chemical, or water spray. Avoid dust clouds. |
| Spill (S6) | Sweep or vacuum carefully. Avoid dust generation. Collect in labelled container. |
| PPE (S8) | Safety glasses; nitrile gloves; lab coat; P1 respirator for bulk weighing |
Sections 9–15 Summary
| Physical state | Brown to dark brown powder, characteristic meaty odour |
| Stability (S10) | Stable. Hygroscopic. Keep dry and tightly closed. |
| Toxicology (S11) | Not classified as acutely toxic. Low toxicity at normal laboratory exposure. |
| Ecology (S12) | High organic load — avoid large-scale discharge to waterways. Readily biodegradable. |
| Disposal (S13) | Via licensed waste contractor or institutional waste system per local EPA requirements. |
| Transport (S14) | Not dangerous goods — ADG, IMDG, IATA |
| Regulatory (S15) | Not a hazardous substance (WHS 2023). Not a scheduled poison (SUSMP AU). AICIS compliant. Bovine origin — verify suitability for TSE/BSE-controlled applications. |
Quality Specifications
| Parameter | Specification | Typical Result | Method |
|---|---|---|---|
| Appearance | Brown to dark brown powder | Conforms | Visual |
| Solubility | Clear to slightly turbid brown solution (5% aq) | Conforms | Visual |
| pH (5% aqueous, 25°C) | 6.0 – 7.5 | 6.8 | pH meter (calibrated) |
| Loss on drying (105°C) | ≤ 6.0% | 3.2% | Loss on drying |
| Total nitrogen (Kjeldahl) | 11.5 – 12.5% | 12.1% | Kjeldahl |
| Amino nitrogen (as N) | 3.5 – 4.5% | 4.0% | Formol titration |
| Sulfated ash (800°C) | ≤ 18.0% | 14.2% | Gravimetric |
| Nitrite (NO₂) | Passes test | Passes | Colorimetric |
| Identity (NIR) | Passes test | Passes | NIR spectroscopy |
| Suitability for microbiology | Passes test — supports growth of test organisms | Passes | ISO 11133 |
| E. coli ATCC 25922 | Good growth | Good growth | QC organism test |
| S. aureus ATCC 25923 | Good growth | Good growth | QC organism test |
Pack Sizes
| 500 g | Trial and small laboratory use |
| 1 kg | Routine laboratory use |
| 5 kg | Regular production and larger facilities |
| 25 kg | Bulk / institutional / manufacturing |
| Custom | Contact support@ausamics.com.au for bulk or tender quantities |
Manufacturing & Documentation
Supplied by AuSaMicS Pty Ltd
31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467
✓ Specifications verified against Merck/Sigma-Aldrich Cat. 103979 reference
✓ Batch QC: pH, loss on drying, total N, amino N, suitability for microbiology
✓ COA, TDS, SDS included with every order
✓ Australian stock — same-week dispatch, no import delays
31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467
✓ Specifications verified against Merck/Sigma-Aldrich Cat. 103979 reference
✓ Batch QC: pH, loss on drying, total N, amino N, suitability for microbiology
✓ COA, TDS, SDS included with every order
✓ Australian stock — same-week dispatch, no import delays
⚠️ For laboratory, research, and quality control use only. Not for therapeutic, veterinary, food, cosmetic, or direct human use. Bovine origin — users working in TSE/BSE-controlled regulatory environments should verify suitability for their specific application.