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Salmonella Detection Media Set | BPW, RVS, MKTTn & XLD
$359.00 AUD
Salmonella Detection Media — Australian Made
Salmonella Detection Media Set — pre-enrichment, dual selective enrichment & selective/differential isolation
One order for the four dehydrated culture media that carry a Salmonella sample through the pre-enrichment, dual selective enrichment and selective/differential plating stages of the ISO 6579-1 workflow — BPW, RVS Broth, MKTTn Broth Base and XLD Agar. Full documentation on every item.
Manufactured in Melbourne Fast Australian dispatch SDS · TDS · COA per product LOT & EXP traceability Direct formulator support
Where each medium fits in the workflow
1. Pre-enrichmentBPW, 37 °C, 16–20 h — resuscitates stressed cells
→
2a. Selective enrichmentRVS Broth, 41.5 °C, 24 h
2b. Selective enrichmentMKTTn Broth, 37 °C, 24 h
→
3. Selective platingXLD Agar, 37 °C, 24 h — presumptive colonies
Salmonella Detection Media Set
Culture media for the pre-enrichment, dual selective enrichment, and selective/differential isolation stages of Salmonella detection. Designed to support the culture-media stages of the ISO 6579-1 workflow — see "What this set does and doesn't cover" in the FAQ below.
$359 $378 individually Save $19 as a set
Includes
- BPW 500 g — AS-1160
- RVS Broth 500 g — AS-1338
- MKTTn Broth Base 500 g — AS-1301
- XLD Agar 500 g — AS-1378
Before you order: MKTTn Broth Base requires two additions immediately before use — Lugol's Solution 5% and Novobiocin Sodium Salt. Neither is heat-stable, so neither can be pre-mixed into the dehydrated base — they're sold separately below and must be added by the user just before inoculation.
Add Salmonella Media Set — $359
Need different pack sizes — e.g. 10 kg BPW with 500 g XLD? Configure this set for your laboratory →
Colour key: Pre-enrichment Selective enrichment Selective/differential plating
Exact products & quantities
| Product | Code | Pack size |
|---|---|---|
| Buffered Peptone Water (BPW) | AS-1160 | 500 g |
| RVS Broth (Rappaport-Vassiliadis Soya) | AS-1338 | 500 g |
| MKTTn Broth Base (Müller-Kauffmann Tetrathionate-Novobiocin) | AS-1301 | 500 g |
| XLD Agar (Xylose Lysine Deoxycholate) | AS-1378 | 500 g |
Bulk pack sizes are also available: BPW in 250 g ($39), 2.5 kg ($309) and 10 kg ($975); RVS Broth in 2.5 kg ($550) and 10 kg ($1,950); MKTTn Broth Base in 250 g ($65) and 2.5 kg ($445) — contact support@ausamics.com for bulk quotes.
Required additives — must be added to MKTTn before use
Required for MKTTn
Lugol's Solution 5% (Iodine/Potassium Iodide)
AS-54002
Added to MKTTn Broth Base immediately before use — not heat-stable, cannot be autoclaved into the base powder. Essential for correct tetrathionate function.
$29 50 mL (100 mL also available)
Add Lugol's Solution →
Required for MKTTn
Novobiocin Sodium Salt
ASA-2035
The "n" in MKTTn. Added after autoclaving (heat-labile) to suppress Gram-positive background flora and competing Proteus spp.
$55 1 g (2.5 g / 5 g / 10 g also available)
Add Novobiocin →
Recommended complementary media — for plating & confirmation
Second Plating Medium
Brilliant Green Agar (BGA)
AS-1150
ISO 6579-1:2017 calls for streaking each enrichment broth onto XLD plus a second selective/differential agar chosen by the laboratory. Brilliant Green Agar (BGA) is one of the most widely used choices for that second medium — strong inhibition of E. coli/Proteus/Pseudomonas via brilliant green dye and phenol red, giving pink-red Salmonella colonies.
$35 100 g (500 g also available)
Add BGA →
Biochemical Confirmation
TSI Agar (Triple Sugar Iron)
AS-1365
Confirms presumptive colonies from XLD/BGA via glucose/lactose/sucrose fermentation and H2S production pattern, ahead of further biochemical or serological confirmation. Formulated to ISO 6579 / FDA-BAM standards.
$35 100 g (500 g also available)
Add TSI Agar →
Biochemical Confirmation
Urea Broth (Stuart)
AS-1374
High-buffer Stuart's formulation — rules out urease-positive Proteus spp., which can otherwise mimic Salmonella on TSI (H2S-positive, non-lactose fermenting). Salmonella is urease-negative.
$29 100 g (500 g / 2.5 kg / 10 kg also available)
Add Urea Broth →
Why AuSaMicS Salmonella detection media?
Australian-manufacturedManufactured locally in Melbourne for faster supply and reduced reliance on imported finished media.
Fast Australian dispatchManufactured and stocked in Melbourne, dispatched promptly on order.
Full documentationSDS, TDS, and COA for every product in the set — no exceptions.
LOT & EXP traceabilityBatch-coded to support laboratory traceability, documentation and audit requirements.
One order, coordinated setFour complementary dehydrated culture media supplied together for the core Salmonella culture workflow — one order, one delivery.
Direct formulator supportSpeak directly with the chemist. Protocol guidance and custom quantities available on request.
Frequently asked questions
Q1: What does this set cover, and what doesn't it include?
This set covers the culture-media stages of Salmonella detection: pre-enrichment (BPW), dual selective enrichment (RVS and MKTTn run in parallel), and selective/differential plating (XLD). It does not by itself constitute the complete ISO 6579-1 testing and confirmation procedure. MKTTn also requires two additives not included in this set — Lugol's Solution and Novobiocin, both above. Confirmed identification of presumptive colonies requires further biochemical testing (e.g. TSI Agar, Urea Broth) and, where required, serological confirmation — see the complementary media above.
Q2: Why does the workflow use two selective enrichment broths (RVS and MKTTn) instead of one?
RVS Broth and MKTTn Broth have different selective chemistries — RVS relies on magnesium chloride and malachite green at 41.5 °C, while MKTTn relies on tetrathionate, iodine and novobiocin at 37 °C. Different Salmonella serovars and matrices respond better to one broth than the other, so running both in parallel from the same BPW pre-enrichment improves overall recovery.
Q3: What incubation conditions does each medium require?
BPW: 37 °C ± 1 °C for 16–20 hours (pre-enrichment). RVS Broth: 41.5 °C ± 1 °C for 24 ± 3 hours. MKTTn Broth: 37 °C ± 1 °C for 24 ± 3 hours. XLD Agar plates: 37 °C ± 1 °C for 24 hours, read for typical red/pink colonies with black (H2S-positive) centres. ISO 6579-1:2017/Amd 1:2020 broadened several incubation ranges to 34–38 °C — always confirm the exact parameters against your accredited method or the current standard before reporting results.
Q4: Should I plate onto a second selective agar as well as XLD?
Yes — ISO 6579-1:2017 requires streaking each enrichment broth onto XLD plus a second selective/differential agar chosen by the laboratory (not specified by name in the standard). Brilliant Green Agar (BGA) is one of the most commonly used choices and is available as a complementary medium above; Bismuth Sulphite Agar and chromogenic Salmonella agars are other options some laboratories use instead.
Q5: Are individual products available separately?
Yes — all four products are available individually from the AuSaMicS catalogue. Buying them as this set saves approximately 5% versus ordering each item separately. For bulk quantities or custom configurations, contact support@ausamics.com.
Salmonella Detection Media Set — $359
BPW · RVS Broth · MKTTn Broth Base · XLD Agar
Add Salmonella Media Set — $359
Laboratory Use Only — BSL-2 Handling Required. Supplied for in vitro laboratory use by trained personnel; Salmonella spp. is pathogenic and must be handled under Biosafety Level 2 containment. This set covers the culture-media stages of the ISO 6579-1:2017 workflow only — it is not a complete reference method, and results intended for regulatory, export, or public-health reporting should follow a validated, accredited method. Full terms, biosafety guidance and liability information: ausamics.com.au/policies/terms-of-service.