Tryptic Soy Agar (TSA / CASO Agar) | General Growth Medium for Bacteria

Tryptic Soy Agar (TSA / CASO Agar)

General-Purpose Culture Medium for Microbiological Research & Quality Control

Catalog Number: AS-1370

Overview

Tryptic Soy Agar (TSA), also known as CASO Agar, is a nutrient-rich, general-purpose solid culture medium widely used for the cultivation and isolation of a broad range of microorganisms in research, quality control, environmental, and industrial microbiology laboratories.

Its balanced formulation, based on enzymatic digests of casein and soybean meal, provides essential amino acids, peptides, vitamins, and minerals that support the growth of many bacteria, yeasts, and moulds. Due to its versatility and clarity, TSA is commonly used as a baseline medium for routine cultivation, enumeration, and method development.

Ausamics Tryptic Soy Agar is manufactured to deliver consistent performance, clear colony morphology, and reliable batch-to-batch reproducibility.

Key Features & Benefits

• Broad-spectrum growth support for diverse microorganisms

• Nutrient-rich formulation based on casein and soy digests

• Clear, well-defined agar surface for easy colony observation

• Suitable for routine culture, isolation, and enumeration

• Consistent formulation for reproducible results

• Compatible with enrichment or supplementation when required

Typical Applications

Research & Quality Control Laboratories

• General microbial cultivation and maintenance

• Total viable count and enumeration studies

• Routine quality control testing

Environmental & Industrial Microbiology

• Monitoring of environmental samples

• Surface and air sampling studies

• Process hygiene and contamination assessment

Food & Beverage Microbiology (Research & QC)

• Monitoring microbial load in production environments

• Evaluation of general microbial presence in samples

Teaching & Academic Laboratories

• Training in basic microbiological techniques

• Demonstration of general-purpose culture media

Composition (Typical, per litre)

• Tryptone (casein digest): 15.0 g

• Soytone (soybean digest): 5.0 g

• Sodium chloride: 5.0 g

• Agar: 15.0 g

Total: 40.0 g/L

Physical Characteristics

• Appearance (dehydrated): Light beige to cream powder

• Prepared medium: Clear to slightly opalescent amber agar

• Final pH (prepared): Approximately 7.3 ± 0.2 at 25 °C

Preparation

• Suspend 40 g of dehydrated medium in 1 L of distilled or deionised water

• Heat gently with agitation until completely dissolved

• Sterilise by autoclaving at 121 °C for 15 minutes

• Cool to 45–50 °C

• Aseptically dispense into sterile Petri dishes

• Allow agar to solidify at room temperature

Storage & Stability

• Dehydrated medium: Store at 15–25 °C, dry, in a tightly sealed container

• Prepared plates: Store at 2–8 °C, protected from dehydration

Intended Use

For laboratory research, quality control, and educational use only.

Not for human, animal, diagnostic, or therapeutic use.

Quality & Supply Assurance

• Manufactured under controlled conditions for batch consistency

• Certificate of Analysis (COA) available

• Safety Data Sheet (SDS) available upon request

• Bulk quantities and custom packaging available