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X-GlcNAc chromogenic substrate powder 4264-82-8
  • X-GlcNAc chromogenic substrate powder 4264-82-8
X-GlcNAc | 5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-glucosaminide | ASE-908

X-GlcNAc | 5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-glucosaminide | ASE-908

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AuSaMicS Life Science • Enzyme Substrates

X-GlcNAc

5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-glucosaminide

Specialized chromogenic substrate for N-acetyl-β-D-glucosaminidase (NAGase) detection. Critical for yeast identification, fungal diagnostics, and clinical biomarker research with intense blue signal development.

CAS: 4264-82-8 ASE-908 ✓ Australian Stock 🔵 NAGase
🚀 Same-Day Dispatch: Australian Stock 🧪 Clinical Grade: Fungal diagnostics ❄️ Stable: -20°C Storage, Light Protected

🏆 Fungal Diagnostic Specialist

🍄 Yeast Identification - Candida detection
🔬 Clinical Grade - Diagnostic applications
📋 Complete Package - COA + SDS included
⚡ Fast Supply - Ships within 24h
📞 Expert Support - Protocol optimization
Biomarker Research
Essential for NAGase studies

🔬 NAGase Detection Mechanism

X-GlcNAc undergoes N-acetyl-β-D-glucosaminidase-catalyzed hydrolysis, releasing 5-bromo-4-chloro-3-indoxyl which rapidly oxidizes to form an intense blue precipitate:

X-GlcNAc + H₂O
↓ (N-acetyl-β-D-glucosaminidase)
5-Bromo-4-chloro-3-indoxyl + N-acetyl-D-glucosamine
↓ (Spontaneous oxidation & dimerization)
Blue Indigoid Precipitate
Clinical Significance: NAGase activity indicates yeast/fungal presence and metabolic status

📊 Complete Chromogenic Substrate Portfolio

Substrate Target Enzyme Signal Key Organisms Primary Applications
X-Gal β-Galactosidase 🔵 Blue E. coli, Lactobacillus Blue-white screening, molecular cloning
X-Gluc β-Glucuronidase 🔵 Blue E. coli (83%) E. coli detection, GUS reporter
X-GlcNAc N-acetyl-β-D-glucosaminidase 🔵 Blue Candida, yeasts, fungi Fungal identification, clinical diagnostics
Salmon-Gal β-Galactosidase 🔴 Red E. coli, enterobacteria Visual contrast, multiplex assays
Salmon-Caprylate C8 Esterase 🔴 Red Salmonella, Pseudomonas Food safety, pathogen detection
Magenta-Gal β-Galactosidase 🟣 Purple E. coli, enterobacteria Alternative screening, yellow media
ONPG β-Galactosidase 🟡 Yellow Various bacteria Quantitative assays, biochemical tests
MUG β-Glucuronidase 💙 Fluorescent E. coli detection Fluorogenic detection, water testing

🧬 Clinical & Research Applications

🍄 Yeast & Fungal ID

Rapid identification of Candida albicans, C. tropicalis, and other clinically relevant yeasts.

🔬 Clinical Biomarkers

NAGase activity as indicator of tissue damage, inflammation, and disease progression.

Specialized Research Applications:

  • Medical Mycology: Fungal pathogen identification
  • Clinical Chemistry: NAGase as kidney function marker
  • Pharmaceutical: Antifungal drug development
  • Food Safety: Yeast contamination screening
  • Environmental: Fungal ecology studies
  • Biotechnology: Enzyme characterization

🧪 Application-Specific Protocols

Application Concentration Incubation Expected Result
Yeast Identification 50-100 mg/L 35°C, 4-24h Blue colonies (positive)
Clinical Diagnostics 75-150 mg/L 37°C, 2-18h Rapid blue development
Research Applications 25-200 mg/L 25-37°C, 1-72h Dose-dependent intensity
Quality Control 100 mg/L (standard) 35°C, 24h Consistent blue signal

💡 Optimization Guidelines

Stock Solution:
Dissolve in DMSO (10-50 mg/mL)
Working Concentration:
50-100 mg/L in agar medium
pH Optimum:
6.8-7.2 for maximum activity
Storage:
-20°C, desiccated, light protection

📋 Technical Specifications

Chemical Name 5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-glucosaminide
CAS Number 4264-82-8
Formula C₁₆H₁₈BrClN₂O₆
Molecular Weight 449.68 g/mol
Purity ≥98% (HPLC)
Appearance White to pale cream powder
Solubility DMSO, DMF (limited in water)
Storage -20°C, protect from light
HS Code 2934.99.90

🏥 Clinical Significance

  • ✓ Candida Detection: Rapid yeast identification
  • ✓ Kidney Function: NAGase as biomarker
  • ✓ Tissue Damage: Inflammatory indicator
  • ✓ Drug Testing: Antifungal efficacy

✅ Quality Standards

  • ✓ HPLC Purity: ≥98% verified
  • ✓ Enzymatic Activity: Functional validation
  • ✓ Sterility: Microbial testing passed
  • ✓ Heavy Metals: <10 ppm total
  • ✓ Stability: Validated storage

 Identification Kit

Culture Media & Substrates

Yeast Extract Agar

Standard yeast isolation

View Product
Sabouraud Dextrose Agar

Fungal cultivation medium

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Rose Bengal Agar

Selective fungal medium

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CHROMagar Candida

Differential Candida ID

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Antibiotics & Selective Agents

Chloramphenicol

Bacterial inhibition

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Cycloheximide

Fungal selectivity

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Streptomycin

Bacterial suppression

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Gentamicin

Broad spectrum control

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Need Mycology Protocol Support?

Our microbiologists can help optimize your yeast and fungal identification protocols

For laboratory, research, and clinical use only. Not for human consumption or medicinal use.
AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
Same-day dispatch • Australian stock • Full documentation included

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