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X-Gluc (BCIG) | 5-Bromo-4-chloro-3-indolyl β-D-glucuronide CHA salt | ASE-907
  • X-Gluc (BCIG) | 5-Bromo-4-chloro-3-indolyl β-D-glucuronide CHA salt | ASE-907
X-Gluc (CHA Salt) | ASE-907 | Chromogenic GUS / beta-Glucuronidase Substrate

X-Gluc (CHA Salt) | ASE-907 | Chromogenic GUS / beta-Glucuronidase Substrate

$85.00 AUD
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AuSaMicS Life Science • Enzyme Substrates

X-Gluc (X-glucuronide CHA salt)

5-Bromo-4-chloro-3-indolyl β-D-glucuronide

Premium chromogenic substrate for β-glucuronidase detection. Industry standard for E. coli confirmation, GUS reporter assays, and food safety microbiology.

CAS: 114162-64-0 ASE-907 ✓ Australian Stock β-Glucuronidase
Fast Delivery: 1-3 Business Days High Purity: CHA Salt Form Stable Storage: Light Protected

🏆 Quality Assurance

✅ Australian Stock - Ships same day
🧪 CHA Salt Form - High purity substrate
📋 Full Documentation - COA + SDS
🌡️ Proper Storage - Light & moisture protected
📞 Technical Support - Application guidance
Research Grade Quality
Suitable for all β-glucuronidase applications

🔬 Enzymatic Mechanism

X-Gluc serves as a chromogenic substrate for β-glucuronidase (GUS enzyme):

X-Gluc (Colorless) + β-Glucuronidase
↓ (Enzymatic hydrolysis)
5-Bromo-4-chloro-3-indoxyl + Glucuronic acid
↓ (Spontaneous oxidation & dimerization)
Blue Indigoid Precipitate
Detection Result: Positive samples develop intense blue coloration

🧬 Key Applications

🔵 E. coli Detection

Specific identification of E. coli in food, water, and clinical samples based on β-glucuronidase activity.

🧬 GUS Reporter System

Histochemical detection of GUS (uidA) gene expression in plant and microbial systems.

Specialized Applications:

  • Food Safety: Rapid E. coli confirmation in dairy, meat, and produce testing
  • Water Quality: Membrane filtration methods for coliform detection
  • Clinical Diagnostics: UTI pathogen identification
  • Environmental Monitoring: Fecal contamination assessment
  • Molecular Biology: Transgene expression analysis
  • Quality Control: Chromogenic media formulation

⚗️ Chemical Properties

Salt Form:
Cyclohexylammonium (CHA) salt
Advantages:
Enhanced solubility & stability
Storage:
Light & moisture protected
Purity:
High-grade chromogenic media

💡 Application Guidelines

  • Working concentration: 50-100 μg/mL in culture media
  • Incubation: 18-24 hours at 35-37°C for optimal color development
  • pH range: 6.8-7.2 for maximum enzymatic activity
  • Storage conditions: Store dry, dark, at 2-8°C
  • Media compatibility: Compatible with most bacteriological media

📋 Technical Specifications

Chemical Name 5-Bromo-4-chloro-3-indolyl β-D-glucuronide
Salt Form Cyclohexylammonium salt
Formula C₂₀H₂₆BrClN₂O₇
Molecular Weight 521.79 g/mol
Appearance Light-colored crystalline powder
Solubility Water, DMSO, ethanol
Storage 2-8°C, protect from light
Working Concentration 50-100 μg/mL
HS Code 2934.99.90

📝 Alternative Names

X-Gluc
X-GlcA
X-Gluc CHA salt
5-Bromo-4-chloro-3-indolyl β-D-glucopyranosiduronic acid

✅ Quality Standards

  • ✓ Identity Verified by spectroscopic methods
  • ✓ Functional Activity confirmed
  • ✓ Chromatographic Purity tested
  • ✓ Microbial Quality assessed
  • ✓ Stability under recommended storage

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Need Application Support?

Our microbiologists can help optimize your E. coli detection protocols

For laboratory, research, and industrial use only. Not for food, feed, household, cosmetic, therapeutic, or personal use.
AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
Same-day dispatch • Australian stock • Full documentation included

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